PMID- 26475387
OWN - NLM
STAT- MEDLINE
DCOM- 20160815
LR  - 20151017
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 61
IP  - 5
DP  - 2015 Oct 16
TI  - Activity of dental pulp cells in semisolid 3D cultures initiated by transforming 
      growth factor-beta1 and bone morphogenetic protein 2, 4.
PG  - 45-8
AB  - The intention of this study was to investigate the effect of modified 3D culture 
      conditions on dental pulp cells (DPCs). DPCs were isolated from extracted primary 
      molar, premolar, and wisdom teeth. Tooth samples were divided into three groups 
      as control group; plated into methyl cellulose medium without any 
      supplementation, growth factor (GF) group; supplemented with bone morphogenetic 
      proteins (BMP2, BMP4), transforming growth factor—β1 
      (TGF—β1) and growth factor+conditioned medium (GF+CM) group; 
      supplemented with both growth factors and pulp conditioned medium. The DPCs were 
      tested for colony forming ability, proliferation capacity and morphology. The 
      highest colony forming ability was detected in the GF and GF+CM groups of DPCs 
      isolated from wisdom teeth. The proliferation capacity was higher in GF+CM group 
      of DPCs isolated from primary molars, and in GF and GF+CM groups of DPCs isolated 
      from wisdom teeth. Scanning electron microscope (SEM) observation of the wisdom 
      teeth samples showed cell—cell interactions in the GF and GF+CM groups. Our 
      results indicate that growth factors and pulp conditioned medium in methyl 
      cellulose culture created proper environment to follow the behavior of dental 
      cells three—dimensionally.
FAU - Suzergoz, F
AU  - Suzergoz F
AD  - Harran University Department of Biology, Science Art Faculty Sanliurfa Turkey.
FAU - Sepet, E
AU  - Sepet E
AD  - Istanbul University Department of Pedodontics, Dentistry Faculty Istanbul Turkey.
FAU - Erdem, A P
AU  - Erdem AP
AD  - Istanbul University Department of Pedodontics, Dentistry Faculty Istanbul Turkey.
FAU - Cinar, S
AU  - Cinar S
AD  - Istanbul University Department of Immunology, Institute of Experimental Medicine 
      Istanbul Turkey.
FAU - Ikikarakayali, G
AU  - Ikikarakayali G
AD  - Istanbul University Department of Pedodontics, Dentistry Faculty Istanbul Turkey.
FAU - Erdem, M A
AU  - Erdem MA
AD  - Istanbul University Department of Oral Surgery, Dentistry Faculty Istanbul 
      Turkey.
FAU - Gurol, A O
AU  - Gurol AO
AD  - Istanbul University Department of Immunology, Institute of Experimental Medicine 
      Istanbul Turkey ogurol@istanbul.edu.tr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151016
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bicuspid/*cytology
MH  - Bone Morphogenetic Protein 2/*pharmacology
MH  - Cell Communication/*physiology
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Child
MH  - Colony-Forming Units Assay
MH  - Dental Pulp/*cytology
MH  - Humans
MH  - Microscopy, Electron, Scanning
MH  - Molar, Third/*cytology
MH  - Transforming Growth Factor beta1/*pharmacology
MH  - Young Adult
EDAT- 2015/10/18 06:00
MHDA- 2016/08/16 06:00
CRDT- 2015/10/18 06:00
PHST- 2015/08/20 00:00 [received]
PHST- 2015/10/05 00:00 [accepted]
PHST- 2015/10/18 06:00 [entrez]
PHST- 2015/10/18 06:00 [pubmed]
PHST- 2016/08/16 06:00 [medline]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2015 Oct 16;61(5):45-8.