PMID- 26475590
OWN - NLM
STAT- MEDLINE
DCOM- 20160411
LR  - 20200930
IS  - 1522-1539 (Electronic)
IS  - 0363-6135 (Linking)
VI  - 309
IP  - 12
DP  - 2015 Dec 15
TI  - Reduced uterine perfusion pressure decreases functional capillary density in 
      skeletal muscle.
PG  - H2002-7
LID - 10.1152/ajpheart.00641.2015 [doi]
AB  - The purpose of this study was to examine the functional and structural capillary 
      density in the reduced uterine perfusion pressure (RUPP) model, which when 
      performed during pregnancy is an established animal model of preeclampsia. We 
      hypothesized that the RUPP model would be associated with capillary rarefaction 
      and impaired capillary perfusion, which would be more pronounced in the pregnant 
      state. Female Sprague-Dawley rats (n = 32) were randomized to nonpregnancy 
      (Nonpregnant) or breeding (Pregnant) at 12 wk of age and again to RUPP or SHAM 
      surgeries on gestational day (GD) 14 (or equivalent age in nonpregnant rats). On 
      GD 20 (or equivalent), capillary structure and perfusion of the extensor 
      digitorum longus were imaged using digital intravital video microscopy. 
      Functional videos were analyzed by a blinded observer to measure capillary 
      density, expressed as capillaries per millimeter intersecting three staggered 
      reference lines (200 mum). Flow was scored as the percentage of capillaries having 
      1) continuous, 2) intermittent, or 3) stopped flow. Total capillary density was 
      not different between groups. There was a main effect of RUPP surgery resulting 
      in decreased continuous flow vessels (P < 0.01) and increased stopped flow (P < 
      0.01), which was driven by differences between pregnant animals (Continuous flow: 
      pregnant SHAM 80.1 +/- 7.8% vs. pregnant RUPP 67.8 +/- 11.2%, P < 0.05) (Stopped 
      flow: pregnant SHAM 8.7 +/- 3.2% vs. pregnant RUPP 17.9 +/- 5.7%, P < 0.01). Our 
      results demonstrate that the RUPP surgery is associated with a decrease in 
      functional capillary density in skeletal muscle that is more pronounced in the 
      pregnant state, which may contribute to the vascular pathophysiology observed in 
      preeclampsia.
CI  - Copyright (c) 2015 the American Physiological Society.
FAU - Fraser, Graham M
AU  - Fraser GM
AD  - Department of Medical Biophysics, Schulich School of Medicine and Dentistry, 
      Western University, London, Ontario, Canada;
FAU - Morton, Jude S
AU  - Morton JS
AD  - Department of Obstetrics & Gynecology, Faculty of Medicine & Dentistry, 
      University of Alberta, Edmonton, Alberta, Canada; Women and Children's Health 
      Research Institute, University of Alberta, Edmonton, Alberta, Canada;
FAU - Schmidt, Sydney M
AU  - Schmidt SM
AD  - Women and Children's Health Research Institute, University of Alberta, Edmonton, 
      Alberta, Canada; Program for Pregnancy and Postpartum Health, Physical Activity 
      and Diabetes Laboratory, Faculty of Physical Education and Recreation, University 
      of Alberta, Edmonton, Alberta, Canada; and.
FAU - Bourque, Stephane
AU  - Bourque S
AD  - Department of Anesthesiology and Pain Medicine, University of Alberta, Edmonton, 
      Alberta, Canada; Women and Children's Health Research Institute, University of 
      Alberta, Edmonton, Alberta, Canada;
FAU - Davidge, Sandra T
AU  - Davidge ST
AD  - Department of Obstetrics & Gynecology, Faculty of Medicine & Dentistry, 
      University of Alberta, Edmonton, Alberta, Canada; Women and Children's Health 
      Research Institute, University of Alberta, Edmonton, Alberta, Canada;
FAU - Davenport, Margie H
AU  - Davenport MH
AD  - Women and Children's Health Research Institute, University of Alberta, Edmonton, 
      Alberta, Canada; Program for Pregnancy and Postpartum Health, Physical Activity 
      and Diabetes Laboratory, Faculty of Physical Education and Recreation, University 
      of Alberta, Edmonton, Alberta, Canada; and Alberta Diabetes Institute, University 
      of Alberta, Edmonton, Alberta, Canada.
FAU - Steinback, Craig D
AU  - Steinback CD
AD  - Women and Children's Health Research Institute, University of Alberta, Edmonton, 
      Alberta, Canada; Program for Pregnancy and Postpartum Health, Physical Activity 
      and Diabetes Laboratory, Faculty of Physical Education and Recreation, University 
      of Alberta, Edmonton, Alberta, Canada; and Alberta Diabetes Institute, University 
      of Alberta, Edmonton, Alberta, Canada craig.steinback@ualberta.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151016
PL  - United States
TA  - Am J Physiol Heart Circ Physiol
JT  - American journal of physiology. Heart and circulatory physiology
JID - 100901228
SB  - IM
MH  - Animals
MH  - Blood Pressure/*physiology
MH  - Capillaries/anatomy & histology/*physiology
MH  - Disease Models, Animal
MH  - Female
MH  - Hindlimb/surgery
MH  - Microscopy, Video
MH  - Models, Biological
MH  - Muscle, Skeletal/anatomy & histology/*blood supply
MH  - Pre-Eclampsia/physiopathology
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Regional Blood Flow
MH  - Uterus/anatomy & histology/*blood supply/surgery
OTO - NOTNLM
OT  - RUPP model
OT  - capillary function
OT  - preeclampsia
OT  - pregnancy
EDAT- 2015/10/18 06:00
MHDA- 2016/04/12 06:00
CRDT- 2015/10/18 06:00
PHST- 2015/08/14 00:00 [received]
PHST- 2015/10/13 00:00 [accepted]
PHST- 2015/10/18 06:00 [entrez]
PHST- 2015/10/18 06:00 [pubmed]
PHST- 2016/04/12 06:00 [medline]
AID - ajpheart.00641.2015 [pii]
AID - 10.1152/ajpheart.00641.2015 [doi]
PST - ppublish
SO  - Am J Physiol Heart Circ Physiol. 2015 Dec 15;309(12):H2002-7. doi: 
      10.1152/ajpheart.00641.2015. Epub 2015 Oct 16.