PMID- 26477931
OWN - NLM
STAT- MEDLINE
DCOM- 20160817
LR  - 20170813
IS  - 1567-7257 (Electronic)
IS  - 1567-1348 (Linking)
VI  - 36
DP  - 2015 Dec
TI  - A comparative study on worldwide genetic diversity and population structure 
      analysis of Plasmodium vivax thrombospondin-related adhesive protein (PvTRAP) and 
      its implications for the vivax vaccine design.
PG  - 410-423
LID - S1567-1348(15)30015-0 [pii]
LID - 10.1016/j.meegid.2015.10.015 [doi]
AB  - Plasmodium vivax thrombospondin-related anonymous protein (PvTRAP) is a promising 
      malaria vaccine candidate; however, it exhibits sequence heterogeneity. 
      Therefore, to design a broadly protective vivax vaccine, it is essential to have 
      adequate information on signatures of selection and geospatial genetic diversity 
      of global PvTRAP. For this purpose, 50 Iranian pvtrap were sequenced and compared 
      with related available global sequences in GenBank. The nucleotide sequence 
      analysis of Iranian pvtrap in comparison with the Sal-1 sequence showed the 
      occurrence of 15 SNPs, and all sites were dimorphic. In total, 12 amino acid 
      substitutions were detected and 2 of which were novel, resulting in 10 haplotypes 
      that 8 of them were not reported in any other geographic regions. In comparison 
      with global population, haplotype and nucleotide diversities were lowest in South 
      Korean populations while higher levels of diversities were observed in Thai and 
      Brazilian P. vivax populations. All 12 amino acid replacements in ectodomain of 
      Iranian PvTRAP were distributed in predicted either B- or T-cells epitope as well 
      as intrinsically unstructured/disordered regions (IURs). The present results 
      revealed that observing the relatively low-level diversity in PvTRAP protein 
      might actually be selected by immune response. In summary, the present analysis 
      in parallel to the limited available published data has shown that genetic 
      diversity in the global pvtrap exhibits low-level diversity and geographic 
      variation. These results are of practical significance for the strategic 
      development and deployment of control measures in particular for development of 
      PvTRAP-based malaria vaccine.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Nazeri, Saeed
AU  - Nazeri S
AD  - Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), 
      Pasteur Institute of Iran, Tehran, Iran, Pasteur Avenue, P.O. Box 1316943551, 
      Tehran, Iran.
FAU - Mehrizi, Akram Abouie
AU  - Mehrizi AA
AD  - Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), 
      Pasteur Institute of Iran, Tehran, Iran, Pasteur Avenue, P.O. Box 1316943551, 
      Tehran, Iran.
FAU - Djadid, Navid Dinparast
AU  - Djadid ND
AD  - Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), 
      Pasteur Institute of Iran, Tehran, Iran, Pasteur Avenue, P.O. Box 1316943551, 
      Tehran, Iran.
FAU - Zakeri, Sedigheh
AU  - Zakeri S
AD  - Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), 
      Pasteur Institute of Iran, Tehran, Iran, Pasteur Avenue, P.O. Box 1316943551, 
      Tehran, Iran. Electronic address: zakeris@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151023
PL  - Netherlands
TA  - Infect Genet Evol
JT  - Infection, genetics and evolution : journal of molecular epidemiology and 
      evolutionary genetics in infectious diseases
JID - 101084138
RN  - 0 (DNA, Protozoan)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Malaria Vaccines)
RN  - 0 (Peptides)
RN  - 0 (Protozoan Proteins)
RN  - 120300-02-9 (thrombospondin-related adhesive protein, protozoan)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - DNA, Protozoan
MH  - Epitopes, T-Lymphocyte/chemistry/immunology
MH  - Evolution, Molecular
MH  - *Genetic Variation
MH  - *Genetics, Population
MH  - Humans
MH  - Malaria Vaccines/*genetics/immunology
MH  - Malaria, Vivax/*parasitology/prevention & control
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Peptides/chemistry/immunology
MH  - Phylogeny
MH  - Plasmodium vivax/classification/*genetics/immunology
MH  - Polymorphism, Single Nucleotide
MH  - Protein Conformation
MH  - Protozoan Proteins/chemistry/*genetics/immunology
MH  - Recombination, Genetic
MH  - Selection, Genetic
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Genetic diversity
OT  - Iran
OT  - Plasmodium vivax
OT  - Population structure
OT  - Thrombospondin-related anonymous protein
OT  - Vaccine
EDAT- 2015/10/20 06:00
MHDA- 2016/08/18 06:00
CRDT- 2015/10/20 06:00
PHST- 2015/08/12 00:00 [received]
PHST- 2015/10/07 00:00 [revised]
PHST- 2015/10/12 00:00 [accepted]
PHST- 2015/10/20 06:00 [entrez]
PHST- 2015/10/20 06:00 [pubmed]
PHST- 2016/08/18 06:00 [medline]
AID - S1567-1348(15)30015-0 [pii]
AID - 10.1016/j.meegid.2015.10.015 [doi]
PST - ppublish
SO  - Infect Genet Evol. 2015 Dec;36:410-423. doi: 10.1016/j.meegid.2015.10.015. Epub 
      2015 Oct 23.