PMID- 26478434
OWN - NLM
STAT- MEDLINE
DCOM- 20160503
LR  - 20201209
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 467
IP  - 4
DP  - 2015 Nov 27
TI  - Set1/MLL complex is indispensable for the transcriptional ability of heat shock 
      transcription factor 2.
PG  - 805-12
LID - S0006-291X(15)30760-9 [pii]
LID - 10.1016/j.bbrc.2015.10.061 [doi]
AB  - Heat shock transcription factor 2 (HSF2) is one of four mammalian HSFs, and it is 
      essential in neurogenesis and gametogenesis. However, other aspects of this 
      transcription factor have not been thoroughly characterized. We recently 
      demonstrated that HSF2 suppresses the aggregation caused by polyglutamine (polyQ) 
      protein, and that the cell protective ability of HSF2 is mediated through the 
      induction of the small HSP alphaB-crystallin (CRYAB). In the present study, we 
      investigated the mechanism of HSF2-induced CRYAB expression. We demonstrated that 
      HSF2 interacted with the core component of the Set1/MLL H3K4 histone 
      methyltransferase complex, WDR5. Indeed, HSF2 up-regulated the H3K4me3, H3K14Ac, 
      and H3K27Ac (active histone marks) of the CRYAB promoter. WDR5 bound to the HSF2 
      central domain (Domain X) in vitro and in vivo, and Cys278 of HSF2 was 
      indispensable for HSF2-WDR5 interaction. HSF2 also interacted with the Set1/MLL 
      complex. These results suggest that the interaction with the Set1/MLL complex via 
      binding to WDR5 is critical for the transcriptional ability of HSF2.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Hayashida, Naoki
AU  - Hayashida N
AD  - Department of Biochemistry, Yamaguchi University School of Medicine, 1-1-1 
      Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan. Electronic address: 
      hayasida@yamaguchi-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151019
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CRYAB protein, human)
RN  - 0 (Chromatin)
RN  - 0 (Cryab protein, mouse)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HSF4 protein, human)
RN  - 0 (Heat Shock Transcription Factors)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Histones)
RN  - 0 (Hsf2 protein, mouse)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (WDR5 protein, human)
RN  - 0 (alpha-Crystallin B Chain)
RN  - 142297-91-4 (HSF2 protein, human)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
RN  - EC 2.1.1.43 (Kmt2a protein, mouse)
RN  - EC 2.1.1.43 (Setd1A protein, human)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Base Sequence
MH  - Chromatin/metabolism
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - HEK293 Cells
MH  - Heat Shock Transcription Factors
MH  - Heat-Shock Proteins/genetics/*metabolism
MH  - Histone-Lysine N-Methyltransferase/genetics/*metabolism
MH  - Histones/metabolism
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Lysine/metabolism
MH  - Molecular Sequence Data
MH  - Multiprotein Complexes
MH  - Myeloid-Lymphoid Leukemia Protein/genetics/*metabolism
MH  - Recombinant Proteins/genetics/metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - alpha-Crystallin B Chain/genetics/metabolism
OTO - NOTNLM
OT  - Acetylation
OT  - HSF2
OT  - Histone
OT  - Set1/MLL complex
OT  - Trimethylation
OT  - WDR5
EDAT- 2015/10/20 06:00
MHDA- 2016/05/04 06:00
CRDT- 2015/10/20 06:00
PHST- 2015/09/27 00:00 [received]
PHST- 2015/10/11 00:00 [accepted]
PHST- 2015/10/20 06:00 [entrez]
PHST- 2015/10/20 06:00 [pubmed]
PHST- 2016/05/04 06:00 [medline]
AID - S0006-291X(15)30760-9 [pii]
AID - 10.1016/j.bbrc.2015.10.061 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2015 Nov 27;467(4):805-12. doi: 
      10.1016/j.bbrc.2015.10.061. Epub 2015 Oct 19.