PMID- 26484460
OWN - NLM
STAT- MEDLINE
DCOM- 20160210
LR  - 20220317
IS  - 1526-7598 (Electronic)
IS  - 0003-2999 (Linking)
VI  - 121
IP  - 5
DP  - 2015 Nov
TI  - Polydatin Inhibits Mitochondrial Dysfunction in the Renal Tubular Epithelial 
      Cells of a Rat Model of Sepsis-Induced Acute Kidney Injury.
PG  - 1251-60
LID - 10.1213/ANE.0000000000000977 [doi]
AB  - BACKGROUND: Mitochondrial injury is a major cause of sepsis-induced organ 
      failure. Polydatin (PD), a natural polyphenol, demonstrates protective 
      mitochondrial effects in neurons and arteriolar smooth muscle cells during severe 
      shock. In this study, we investigated the effects of PD on renal tubular 
      epithelial cell (RTEC) mitochondria in a rat model of sepsis-induced acute kidney 
      injury. METHODS: Rats underwent cecal ligation and puncture (CLP) to mimic 
      sepsis-induced acute kidney injury. Rats were randomly divided into sham, CLP + 
      normal saline, CLP + vehicle, and CLP + PD groups. Normal saline, vehicle, and 30 
      mg/kg PD were administered at 6, 12, and 18 hours after CLP or sham surgery via 
      the tail vein. Mitochondrial morphology, metabolism, and function in RTECs were 
      then assessed. Serum cytokines, renal function, survival, and histologic changes 
      in the kidney were also evaluated. RESULTS: CLP increased lipid peroxide content, 
      lysosomal instability, and opening of the mitochondrial permeability transition 
      pore and caused mitochondrial swelling. Moreover, mitochondrial membrane 
      potential (DeltaPsim) was decreased and ATP levels reduced after CLP. PD inhibited all 
      the above effects. It also inhibited the inflammatory response, improved renal 
      function, attenuated histologic indicators of kidney damage, and prolonged 
      survival. CONCLUSIONS: PD protects RTECs against mitochondrial dysfunction and 
      prolongs survival in a rat model of sepsis-induced acute kidney injury. These 
      effects may partially result from reductions in interleukin-6 and oxidative 
      stress.
FAU - Gao, Youguang
AU  - Gao Y
AD  - From the *Department of Critical Care Medicine, Nanfang Hospital, Southern 
      Medical University, Guangzhou, Guangdong Province, P.R. China; daggerDepartment of 
      Anesthesiology, The First Affiliated Hospital of Fujian Medical University, 
      Fuzhou, Fujian Province, P.R. China; double daggerDepartment of Pathophysiology, Southern 
      Medical University, Guangzhou, Guangdong Province, P.R. China;  section signDepartment of 
      Critical Care Medicine, The First People's Hospital of Chenzhou, Chenzhou, Hunan, 
      China;  parallelInstitute of Translation Medicine, University of South China, Hunan 
      Province, China; and  paragraph signDepartment of Pathology, Maternal and Child Health Hospital 
      of Liuzhou, Liu Zhou, Guangxi Province, P.R. China.
FAU - Zeng, Zhenhua
AU  - Zeng Z
FAU - Li, Tao
AU  - Li T
FAU - Xu, Siqi
AU  - Xu S
FAU - Wang, Xingmin
AU  - Wang X
FAU - Chen, Zhongqing
AU  - Chen Z
FAU - Lin, Caizhu
AU  - Lin C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anesth Analg
JT  - Anesthesia and analgesia
JID - 1310650
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Glucosides)
RN  - 0 (Stilbenes)
RN  - XM261C37CQ (polydatin)
SB  - IM
MH  - Acute Kidney Injury/*drug therapy/etiology/pathology
MH  - Animals
MH  - *Disease Models, Animal
MH  - Drugs, Chinese Herbal/pharmacology/therapeutic use
MH  - Epithelial Cells/*drug effects/pathology/physiology
MH  - Glucosides/pharmacology/*therapeutic use
MH  - Kidney Tubules/*drug effects/pathology/physiology
MH  - Membrane Potential, Mitochondrial/drug effects/physiology
MH  - Mitochondria/*drug effects/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sepsis/complications/*drug therapy/pathology
MH  - Stilbenes/pharmacology/*therapeutic use
EDAT- 2015/10/21 06:00
MHDA- 2016/02/11 06:00
CRDT- 2015/10/21 06:00
PHST- 2015/10/21 06:00 [entrez]
PHST- 2015/10/21 06:00 [pubmed]
PHST- 2016/02/11 06:00 [medline]
AID - 00000539-201511000-00021 [pii]
AID - 10.1213/ANE.0000000000000977 [doi]
PST - ppublish
SO  - Anesth Analg. 2015 Nov;121(5):1251-60. doi: 10.1213/ANE.0000000000000977.