PMID- 26485919
OWN - NLM
STAT- MEDLINE
DCOM- 20160915
LR  - 20181202
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 35
IP  - 8
DP  - 2015 Aug
TI  - [Effect of Herba Lycopodii Alcohol Extracted Granule Combined Methylprednisolone 
      on Expression Levels of BDNF and NMDA and Behavior of Traumatic Spinal Cord 
      Injury Rats].
PG  - 1004-10
AB  - OBJECTIVE: To study different effects of Herba Lycopodii (HL) Alcohol Extracted 
      Granule combined methylprednisolone on behavioral changes, brain derived 
      neurotrophic factor (BDNF) expression levels, and N-methyl-D-aspartate (NMDA) 
      receptor levels in rats with spinal cord injury (SCI). METHODS: Male adult SD 
      rats were randomly divided into five groups, i.e., the sham-operation group, the 
      model group, the HL treatment group, the methylprednisolone treatment group, the 
      HL + methylprednisolone treatment group. Rats in the HL treatment group were 
      intragastrically administered with HL at the daily dose of 50 mg/kg for 5 
      successive days. Rats in the methylprednisolone treatment group were 
      intramuscularly injected with 50 mg/kg methylprednisolone within 8 h after spinal 
      cord contusion, and then the dose of methylprednisolone was reduced for 10 mg/kg 
      for 5 successive days. Rats in the HL + methylprednisolone treatment group 
      received the two methods used for the aforesaid two groups. Basso Beattie and 
      Bresnahan (BBB) score (for hindlimb motor functions) were assessed at day 0, 3, 
      7, and 28 after operation. At day 13 after SCI, injured spinal T8-10 was taken 
      from 8 rats of each group and stored in liquid nitrogen. The N-methyl-D-aspartate 
      (NMDA) receptor affinity (Kd) and the maximal binding capacity (Bmax) were 
      determined using [3H]MK-801 radioactive ligand assay. Rats' injured spinal cords 
      were taken for immunohistochemical assay at day 28 after SCI. Expression levels 
      of BDNF in the ventral and dorsal horn of the spinal cord were observed. RESULTS: 
      Compared with the sham-operation group, the number of BDNF positive neurons in 
      the ventral and dorsal horn of the spinal cord increased in the model group, Bmax 
      increased (470 +/- 34), Kd decreased, and BBB scores decreased at day 3 -28 (all P 
      <0. 05). Compared with the SCI model group, the number of BDNF positive neurons 
      and Kd increased, BBB scores at day 3 -28 increased (P <0. 05) in each medicated 
      group. Bmax was (660 +/- 15) in the methylprednisolone treatment group, (646 +/- 25) 
      in the HL treatment group, and (510 +/- 21) in the HL +methylprednisolone treatment 
      group (P <0. 05). Compared with the methylprednisolone treatment group, the 
      number of BDNF positive neurons and Kd increased, BBB scores at day 7 -28 
      increased, and Bmax decreased in the HL treatment group and the HL + 
      methylprednisolone treatment group (all P <0. 05). Compard with the HL treatment 
      group, the number of BDNF positive neurons and Kd increased, and Bmax decreased 
      (all P < 0.05). CONCLUSIONS: HL could effectively improve motor functions of 
      handlimbs, increase expression levels of BDNF in the spinal cord, and lessen 
      secondary injury by affecting spinal levels of NMDA receptors. It showed certain 
      therapeutic and protective roles in treating SCI. Its effect was better than that 
      of methylprednisolone with synergism.
FAU - Xu, Zheng-guang
AU  - Xu ZG
FAU - Yang, Jun
AU  - Yang J
FAU - Lv, Zhi-ping
AU  - Lv ZP
FAU - Wang, Ting-hua
AU  - Wang TH
FAU - Li, Xiao-song
AU  - Li XS
FAU - Liu, Jiang-hua
AU  - Liu JH
FAU - Zhao, Nan
AU  - Zhao N
FAU - Xiyang, Yan-bin
AU  - Xiyang YB
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 3K9958V90M (Ethanol)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - X4W7ZR7023 (Methylprednisolone)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Drugs, Chinese Herbal/*pharmacology/therapeutic use
MH  - Ethanol
MH  - Male
MH  - Methylprednisolone/pharmacology/*therapeutic use
MH  - Models, Animal
MH  - N-Methylaspartate/*metabolism
MH  - Neurons
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate
MH  - Spinal Cord Injuries/*drug therapy/metabolism
EDAT- 2015/10/22 06:00
MHDA- 2016/09/16 06:00
CRDT- 2015/10/22 06:00
PHST- 2015/10/22 06:00 [entrez]
PHST- 2015/10/22 06:00 [pubmed]
PHST- 2016/09/16 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2015 Aug;35(8):1004-10.