PMID- 26487085
OWN - NLM
STAT- MEDLINE
DCOM- 20160531
LR  - 20201209
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 97
IP  - 1
DP  - 2016 Jan
TI  - Amino acid residues Ala283 and His421 in the RNA-dependent RNA polymerase of 
      porcine reproductive and respiratory syndrome virus play important roles in viral 
      ribavirin sensitivity and quasispecies diversity.
PG  - 53-59
LID - 10.1099/jgv.0.000316 [doi]
AB  - The quasispecies diversity of RNA viruses is mainly determined by the fidelity of 
      RNA-dependent RNA polymerase (RdRp) during viral RNA replication. Certain amino 
      acid residues play an important role in determining the fidelity, and such 
      residues can be substituted with other amino acids to produce virus strains with 
      higher fidelity. In this study, two amino acid substitutions (A283T and H421Y) in 
      the RdRp of porcine reproductive and respiratory syndrome virus (PRRSV) were 
      identified under the selection of ribavirin. Preliminary data showed that two 
      substitutions were involved in conferring PRRSV with the properties of increased 
      ribavirin resistance and restricted quasispecies diversity. The results indicated 
      that these two amino acid residues (Ala283 and His421) play a crucial role in 
      PRRSV replication by affecting the fidelity of its RdRp. The results have 
      important implications for understanding the molecular mechanism of PRRSV 
      evolution and pathogenicity, and developing a safer modified live-attenuated 
      vaccine (MLV) against PRRSV.
FAU - Tian, Debin
AU  - Tian D
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University (Virginia Tech), 
      Blacksburg, VA, USA.
FAU - Meng, Xiang-Jin
AU  - Meng XJ
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University (Virginia Tech), 
      Blacksburg, VA, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151019
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Amino Acids)
RN  - 0 (Antiviral Agents)
RN  - 49717AWG6K (Ribavirin)
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
SB  - IM
MH  - Amino Acids/*genetics
MH  - Antiviral Agents/*pharmacology
MH  - *Drug Resistance, Viral
MH  - *Genetic Variation
MH  - Mutation, Missense
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*enzymology/genetics
MH  - RNA-Dependent RNA Polymerase/*genetics
MH  - Ribavirin/*pharmacology
MH  - Selection, Genetic
EDAT- 2015/10/22 06:00
MHDA- 2016/06/01 06:00
CRDT- 2015/10/22 06:00
PHST- 2015/10/22 06:00 [entrez]
PHST- 2015/10/22 06:00 [pubmed]
PHST- 2016/06/01 06:00 [medline]
AID - 10.1099/jgv.0.000316 [doi]
PST - ppublish
SO  - J Gen Virol. 2016 Jan;97(1):53-59. doi: 10.1099/jgv.0.000316. Epub 2015 Oct 19.