PMID- 26487485
OWN - NLM
STAT- MEDLINE
DCOM- 20161021
LR  - 20190610
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 25
IP  - 2
DP  - 2016 Jan 15
TI  - Energy Metabolism in Mesenchymal Stem Cells During Osteogenic Differentiation.
PG  - 114-22
LID - 10.1089/scd.2015.0193 [doi]
AB  - There is emerging interest in stem cell energy metabolism and its effect on 
      differentiation. Bioenergetic changes in differentiating bone marrow mesenchymal 
      stem cells (MSCs) are poorly understood and were the focus of our study. Using 
      bioenergetic profiling and transcriptomics, we have established that MSCs 
      activate the mitochondrial process of oxidative phosphorylation (OxPhos) during 
      osteogenic differentiation, but they maintain levels of glycolysis similar to 
      undifferentiated cells. Consistent with their glycolytic phenotype, 
      undifferentiated MSCs have high levels of hypoxia-inducible factor 1 (HIF-1). 
      Osteogenically induced MSCs downregulate HIF-1 and this downregulation is 
      required for activation of OxPhos. In summary, our work provides important 
      insights on MSC bioenergetics and proposes a HIF-based mechanism of regulation of 
      mitochondrial OxPhos in MSCs.
FAU - Shum, Laura C
AU  - Shum LC
AD  - 1 Center for Musculoskeletal Research, University of Rochester School of Medicine 
      and Dentistry , Rochester, New York.
FAU - White, Noelle S
AU  - White NS
AD  - 1 Center for Musculoskeletal Research, University of Rochester School of Medicine 
      and Dentistry , Rochester, New York.
FAU - Mills, Bradley N
AU  - Mills BN
AD  - 2 Department of Neurology, University of Rochester School of Medicine and 
      Dentistry , Rochester, New York.
FAU - Bentley, Karen L de Mesy
AU  - Bentley KL
AD  - 1 Center for Musculoskeletal Research, University of Rochester School of Medicine 
      and Dentistry , Rochester, New York.
AD  - 3 Department of Pathology, University of Rochester School of Medicine and 
      Dentistry , Rochester, New York.
FAU - Eliseev, Roman A
AU  - Eliseev RA
AD  - 1 Center for Musculoskeletal Research, University of Rochester School of Medicine 
      and Dentistry , Rochester, New York.
AD  - 3 Department of Pathology, University of Rochester School of Medicine and 
      Dentistry , Rochester, New York.
LA  - eng
GR  - K01 AR064610/AR/NIAMS NIH HHS/United States
GR  - P30 AR061307/AR/NIAMS NIH HHS/United States
GR  - R03 AR061515/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20151210
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
SB  - IM
MH  - Bone Marrow Cells/*cytology
MH  - Cell Differentiation/*physiology
MH  - Cell Proliferation/physiology
MH  - Cells, Cultured
MH  - Energy Metabolism/*physiology
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mitochondria/metabolism
MH  - Osteogenesis/*physiology
PMC - PMC4733323
EDAT- 2015/10/22 06:00
MHDA- 2016/10/22 06:00
PMCR- 2017/01/15
CRDT- 2015/10/22 06:00
PHST- 2015/10/22 06:00 [entrez]
PHST- 2015/10/22 06:00 [pubmed]
PHST- 2016/10/22 06:00 [medline]
PHST- 2017/01/15 00:00 [pmc-release]
AID - 10.1089/scd.2015.0193 [pii]
AID - 10.1089/scd.2015.0193 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2016 Jan 15;25(2):114-22. doi: 10.1089/scd.2015.0193. Epub 2015 
      Dec 10.