PMID- 26487642
OWN - NLM
STAT- MEDLINE
DCOM- 20160826
LR  - 20220318
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Linking)
VI  - 40
IP  - 1
DP  - 2016 Jan-Feb
TI  - The Potential of Various Living Tissues for Monitoring Clenbuterol Abuse in 
      Food-Producing Chinese Simmental Beef Cattle.
PG  - 72-7
LID - 10.1093/jat/bkv118 [doi]
AB  - We aimed to evaluate whether living tissues such as urine, plasma and hair were 
      suitable for monitoring clenbuterol (CL) abuse after its subchronic 
      administration of a growth-promoting dose to the Chinese Simmental beef cattle. 
      Eight male, white and red pied Chinese Simmental beef cattle were involved in the 
      experiment, and the CL dose was 16 microg/kg BW/day. Liquid chromatography tandem 
      mass spectrometry (LC-MS-MS) was used to determine CL residues in different 
      tissues, and the addition of D9-clenbuterol internal standard was applied to 
      increase determination accuracy. The recovery of plasma, urine, hair and in vivo 
      tissues was 88.5-114.2, 83.9-114.3, 88.6-116.9 and 85.3-121.7%, respectively. The 
      results showed that CL residue concentrations in the plasma, on Days 14 after 
      withdrawal and later, were lower than the limit of detection (LOD) (0.06 ng/mL) 
      and CL residue in urine was lower than LOD (0.16 ng/mL) 42 days after treatment. 
      CL significantly accumulated in the white and red hair and maintained more than 
      7.19 +/- 2.19 pg/mg within the early withdrawal period of 70 days. A large number 
      of CL were determined in all tested biological tissues, in which residues were 
      higher than the maximum residue limits (MRLs) after dietary administration of CL 
      for 21 days and pre-slaughter withdrawal period of  approximately 6 h. A particular concern is 
      the slow depletion of residues of CL in some tissues like gluteus and liver still 
      exceeding theirs MRLs, respectively, on Days 14 or 28 days after withdrawal. Our 
      study indicated that plasma and urine could be available for monitoring CL abuse 
      only within a short period of time. However, hair (including light-pigmented) as 
      a target matrix can be selected to perform the long-period monitor of CL.
CI  - (c) The Author 2015. Published by Oxford University Press. All rights reserved. For 
      Permissions, please email: journals.permissions@oup.com.
FAU - Li, Lijun
AU  - Li L
AD  - Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 
      Yuanmingyuan West Road, Haidian District, Beijing 100193, P.R. China Scientific 
      Observing and Experiment Station of Animal Genetic Resources and Nutrition in 
      North China, Ministry of Agriculture, Beijing, P.R. China CAAS-ICRAF Joint 
      Laboratory on Agroforestry and Sustainable Animal Husbandry, Beijing, P.R. China.
FAU - Tang, Chaohua
AU  - Tang C
AD  - Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 
      Yuanmingyuan West Road, Haidian District, Beijing 100193, P.R. China Scientific 
      Observing and Experiment Station of Animal Genetic Resources and Nutrition in 
      North China, Ministry of Agriculture, Beijing, P.R. China CAAS-ICRAF Joint 
      Laboratory on Agroforestry and Sustainable Animal Husbandry, Beijing, P.R. China.
FAU - Zhang, Junmin
AU  - Zhang J
AD  - Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 
      Yuanmingyuan West Road, Haidian District, Beijing 100193, P.R. China Scientific 
      Observing and Experiment Station of Animal Genetic Resources and Nutrition in 
      North China, Ministry of Agriculture, Beijing, P.R. China CAAS-ICRAF Joint 
      Laboratory on Agroforestry and Sustainable Animal Husbandry, Beijing, P.R. China 
      zhjmxms@sina.com.
FAU - Zhao, Qingyu
AU  - Zhao Q
AD  - Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 
      Yuanmingyuan West Road, Haidian District, Beijing 100193, P.R. China Scientific 
      Observing and Experiment Station of Animal Genetic Resources and Nutrition in 
      North China, Ministry of Agriculture, Beijing, P.R. China CAAS-ICRAF Joint 
      Laboratory on Agroforestry and Sustainable Animal Husbandry, Beijing, P.R. China.
FAU - Zhang, Kai
AU  - Zhang K
AD  - Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 
      Yuanmingyuan West Road, Haidian District, Beijing 100193, P.R. China Scientific 
      Observing and Experiment Station of Animal Genetic Resources and Nutrition in 
      North China, Ministry of Agriculture, Beijing, P.R. China CAAS-ICRAF Joint 
      Laboratory on Agroforestry and Sustainable Animal Husbandry, Beijing, P.R. China.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151020
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Adrenergic beta-Agonists)
RN  - XTZ6AXU7KN (Clenbuterol)
SB  - IM
MH  - Adrenergic beta-Agonists/*analysis/blood/urine
MH  - Animals
MH  - Cattle
MH  - Chromatography, High Pressure Liquid
MH  - Clenbuterol/*analysis/blood/urine
MH  - Drug Residues/*analysis
MH  - Foodborne Diseases/prevention & control
MH  - Hair/*chemistry
MH  - Limit of Detection
MH  - Male
MH  - Red Meat/toxicity
MH  - Substance Abuse Detection/*methods/*veterinary
MH  - Tandem Mass Spectrometry
EDAT- 2015/10/22 06:00
MHDA- 2016/08/27 06:00
CRDT- 2015/10/22 06:00
PHST- 2015/10/22 06:00 [entrez]
PHST- 2015/10/22 06:00 [pubmed]
PHST- 2016/08/27 06:00 [medline]
AID - bkv118 [pii]
AID - 10.1093/jat/bkv118 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2016 Jan-Feb;40(1):72-7. doi: 10.1093/jat/bkv118. Epub 2015 Oct 
      20.