PMID- 26491222
OWN - NLM
STAT- MEDLINE
DCOM- 20160627
LR  - 20181113
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2015
DP  - 2015
TI  - Anti-Inflammatory Effects of a Methanol Extract from the Marine Sponge Geodia 
      cydonium on the Human Breast Cancer MCF-7 Cell Line.
PG  - 204975
LID - 10.1155/2015/204975 [doi]
LID - 204975
AB  - Many research groups are working to find new possible anti-inflammatory 
      molecules, and marine sponges represent a rich source of biologically active 
      compounds with pharmacological applications. In the present study, we tested 
      different concentrations of the methanol extract from the marine sponge, Geodia 
      cydonium, on normal human breast epithelial cells (MCF-10A) and human breast 
      cancer cells (MCF-7). Our results show that this extract has no cytotoxic effects 
      on both cell lines whereas it induces a decrease in levels of VEGF and five 
      proinflammatory cytokines (CCL2, CXCL8, CXCL10, IFN-gamma, and TNF-alpha) only in MCF-7 
      cells in a dose-dependent manner, thereby indicating an anti-inflammatory effect. 
      Moreover, interactomic analysis suggests that all six cytokines are involved in a 
      network and are connected with some HUB nodes such as NF-kB subunits and ESR1 
      (estrogen receptor 1). We also report a decrease in the expression of two NFKB1 
      and c-Rel subunits by RT-qPCR experiments only in MCF-7 cells after extract 
      treatment, confirming NF-kB inactivation. These data highlight the potential of 
      G. cydonium for future drug discovery against major diseases, such as breast 
      cancer.
FAU - Costantini, Susan
AU  - Costantini S
AD  - CROM, Istituto Nazionale Tumori "Fondazione G. Pascale", IRCCS, 80131 Napoli, 
      Italy.
FAU - Romano, Giovanna
AU  - Romano G
AD  - Department of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Villa 
      Comunale, 80121 Napoli, Italy.
FAU - Rusolo, Fabiola
AU  - Rusolo F
AD  - CROM, Istituto Nazionale Tumori "Fondazione G. Pascale", IRCCS, 80131 Napoli, 
      Italy.
FAU - Capone, Francesca
AU  - Capone F
AD  - CROM, Istituto Nazionale Tumori "Fondazione G. Pascale", IRCCS, 80131 Napoli, 
      Italy.
FAU - Guerriero, Eliana
AU  - Guerriero E
AD  - CROM, Istituto Nazionale Tumori "Fondazione G. Pascale", IRCCS, 80131 Napoli, 
      Italy.
FAU - Colonna, Giovanni
AU  - Colonna G
AD  - Servizio di Informatica Medica, Azienda Ospedaliera Universitaria, Seconda 
      Universita di Napoli, 80138 Napoli, Italy.
FAU - Ianora, Adrianna
AU  - Ianora A
AD  - Department of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Villa 
      Comunale, 80121 Napoli, Italy.
FAU - Ciliberto, Gennaro
AU  - Ciliberto G
AD  - Direzione Scientifica, Istituto Nazionale Tumori "Fondazione G. Pascale", IRCCS, 
      80131 Napoli, Italy.
FAU - Costantini, Maria
AU  - Costantini M
AD  - Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton 
      Dohrn, Villa Comunale, 80121 Napoli, Italy.
LA  - eng
PT  - Journal Article
DEP - 20150927
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Animals
MH  - Breast Neoplasms/metabolism
MH  - Cell Survival/drug effects
MH  - Female
MH  - Geodia/*chemistry
MH  - Humans
MH  - MCF-7 Cells
MH  - Methanol/*chemistry
PMC - PMC4600500
EDAT- 2015/10/23 06:00
MHDA- 2016/06/28 06:00
PMCR- 2015/09/27
CRDT- 2015/10/23 06:00
PHST- 2014/11/16 00:00 [received]
PHST- 2015/01/19 00:00 [revised]
PHST- 2015/01/20 00:00 [accepted]
PHST- 2015/10/23 06:00 [entrez]
PHST- 2015/10/23 06:00 [pubmed]
PHST- 2016/06/28 06:00 [medline]
PHST- 2015/09/27 00:00 [pmc-release]
AID - 10.1155/2015/204975 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2015;2015:204975. doi: 10.1155/2015/204975. Epub 2015 Sep 27.