PMID- 26491693
OWN - NLM
STAT- MEDLINE
DCOM- 20160801
LR  - 20181113
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2015
DP  - 2015
TI  - Repair of Cranial Bone Defects Using rhBMP2 and Submicron Particle of Biphasic 
      Calcium Phosphate Ceramics with Through-Hole.
PG  - 926291
LID - 10.1155/2015/926291 [doi]
LID - 926291
AB  - Recently a submicron particle of biphasic calcium phosphate ceramic (BCP) with 
      through-hole (donut-shaped BCP (d-BCP)) was developed for improving the 
      osteoconductivity. This study was performed to examine the usefulness of d-BCP 
      for the delivery of osteoinductive rhBMP2 and the effectiveness on cranial bone 
      regeneration. The d-BCP was soaked in rhBMP2 solution and then freeze-dried. 
      Scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), and 
      Raman spectroscopy analyses confirmed that rhBMP2 was well delivered onto the 
      d-BCP surface and the through-hole. The bioactivity of the rhBMP2/d-BCP composite 
      was validated in MC3T3-E1 cells as an in vitro model and in critical-sized 
      cranial defects in C57BL/6 mice. When freeze-dried d-BCPs with rhBMP2 were placed 
      in transwell inserts and suspended above MC3T3-E1, alkaline phosphatase activity 
      and osteoblast-specific gene expression were increased compared to 
      non-rhBMP2-containing d-BCPs. For evaluating in vivo effectiveness, freeze-dried 
      d-BCPs with or without rhBMP2 were implanted into critical-sized cranial defects. 
      Microcomputed tomography and histologic analysis showed that rhBMP2-containing 
      d-BCPs significantly enhanced cranial bone regeneration compared to 
      non-rhBMP2-containing control. These results suggest that a combination of d-BCP 
      and rhBMP2 can accelerate bone regeneration, and this could be used to develop 
      therapeutic strategies in hard tissue healing.
FAU - Jeong, Byung-Chul
AU  - Jeong BC
AD  - Research Center for Biomineralization Disorders and Department of Pharmacology 
      and Dental Therapeutics, School of Dentistry, Chonnam National University, 
      Gwangju 500-757, Republic of Korea.
FAU - Choi, Hyuck
AU  - Choi H
AD  - Research Center for Biomineralization Disorders and Department of Pharmacology 
      and Dental Therapeutics, School of Dentistry, Chonnam National University, 
      Gwangju 500-757, Republic of Korea.
FAU - Hur, Sung-Woong
AU  - Hur SW
AD  - Research Center for Biomineralization Disorders and Department of Pharmacology 
      and Dental Therapeutics, School of Dentistry, Chonnam National University, 
      Gwangju 500-757, Republic of Korea.
FAU - Kim, Jung-Woo
AU  - Kim JW
AD  - Research Center for Biomineralization Disorders and Department of Pharmacology 
      and Dental Therapeutics, School of Dentistry, Chonnam National University, 
      Gwangju 500-757, Republic of Korea.
FAU - Oh, Sin-Hye
AU  - Oh SH
AD  - Research Center for Biomineralization Disorders and Department of Pharmacology 
      and Dental Therapeutics, School of Dentistry, Chonnam National University, 
      Gwangju 500-757, Republic of Korea.
FAU - Kim, Hyun-Seung
AU  - Kim HS
AD  - RIS Foundation for Advanced Biomaterials, Chonnam National University, Gwangju 
      500-757, Republic of Korea.
FAU - Song, Soo-Chang
AU  - Song SC
AD  - Center for Biomaterials, Korea Institute of Science & Technology, Seoul 130-650, 
      Republic of Korea.
FAU - Lee, Keun-Bae
AU  - Lee KB
AD  - Department of Orthopedic Surgery, Chonnam National University Medical School and 
      Hospital, Gwangju 501-757, Republic of Korea.
FAU - Park, Kwang-Bum
AU  - Park KB
AD  - Megagen Implant, Gyeongsan, Gyeongbuk 712-850, Republic of Korea.
FAU - Koh, Jeong-Tae
AU  - Koh JT
AD  - Research Center for Biomineralization Disorders and Department of Pharmacology 
      and Dental Therapeutics, School of Dentistry, Chonnam National University, 
      Gwangju 500-757, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150927
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Hydroxyapatites)
RN  - 0 (Recombinant Proteins)
RN  - 0 (hydroxyapatite-beta tricalcium phosphate)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Protein 2/*pharmacology
MH  - Bone Regeneration/*drug effects
MH  - Ceramics/*pharmacology
MH  - Humans
MH  - Hydroxyapatites/*pharmacology
MH  - Male
MH  - Mice
MH  - Recombinant Proteins/pharmacology
MH  - Skull/*injuries
PMC - PMC4600556
EDAT- 2015/10/23 06:00
MHDA- 2016/08/02 06:00
PMCR- 2015/09/27
CRDT- 2015/10/23 06:00
PHST- 2015/04/22 00:00 [received]
PHST- 2015/06/24 00:00 [accepted]
PHST- 2015/10/23 06:00 [entrez]
PHST- 2015/10/23 06:00 [pubmed]
PHST- 2016/08/02 06:00 [medline]
PHST- 2015/09/27 00:00 [pmc-release]
AID - 10.1155/2015/926291 [doi]
PST - ppublish
SO  - Biomed Res Int. 2015;2015:926291. doi: 10.1155/2015/926291. Epub 2015 Sep 27.