PMID- 2649174
OWN - NLM
STAT- MEDLINE
DCOM- 19890512
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 73
IP  - 5
DP  - 1989 Apr
TI  - Immune reconstitution following bone marrow transplantation: comparison of 
      recipients of T-cell depleted marrow with recipients of conventional marrow 
      grafts.
PG  - 1340-50
AB  - The reconstitution of hematopoietic cells and in vitro assays of immunologic 
      function have been followed in leukemic patients after conventional bone marrow 
      transplantation (BMT) (N = 34) and T-cell depleted BMT (N = 52) from human 
      leukocyte antigen (HLA)-identical sibling donors. No effects of the T-cell 
      depletion could be seen on the recovery of myeloid or lymphoid cells as measured 
      by the day to engraftment or by the absolute number of cells through day 100. 
      Normal numbers of lytically active natural killer cells returned the earliest and 
      were rapidly followed in both groups of patients by the appearance of circulating 
      B cells and normalization of the responses to B-cell mitogens. However, the 
      recovery of normal T-cell proliferative responses were more delayed in recipients 
      of T-cell depleted grafts. Significant quantitative differences were seen only 
      during the first 3 months after transplantation. Neither the number of CD3+ T 
      cells nor the ratio of CD4:CD8 positive cells differed markedly between the two 
      transplant groups. Mitogen-induced immunoglobulin production by peripheral blood 
      lymphocytes (PBL) from patients following T-cell depleted BMT was quantitatively 
      less than that of conventional marrow recipients through the first year, with low 
      normal IgM production reached by 4 to 6 months in both groups. IgG production 
      reached low normal 7 to 9 months after conventional BMT but did not remain at 
      this level until 1 year following either type of transplant. Assessment of the 
      incidence of infections from the day the absolute neutrophil count reached 500 
      until day 180 after transplant revealed no significant differences between the 
      two groups; indeed, the overall nonleukemic mortality was higher in the 
      recipients of conventional bone marrow. Thus, in our series, the removal of 
      mature cells from the marrow graft did not affect the rate or degree of recovery 
      of myeloid and lymphoid cells but did affect the regeneration of in vitro T-cell 
      dependent functions. We noted early quantitative differences and a delay in the 
      normalization of the T-cell functions measured rather than prolonged absolute 
      deficiencies. The in vitro deficiencies did not result in significant clinically 
      apparent differences between the two groups.
FAU - Keever, C A
AU  - Keever CA
AD  - Bone Marrow Transplantation Service, Memorial Sloan-Kettering Cancer Center, New 
      York 10021.
FAU - Small, T N
AU  - Small TN
FAU - Flomenberg, N
AU  - Flomenberg N
FAU - Heller, G
AU  - Heller G
FAU - Pekle, K
AU  - Pekle K
FAU - Black, P
AU  - Black P
FAU - Pecora, A
AU  - Pecora A
FAU - Gillio, A
AU  - Gillio A
FAU - Kernan, N A
AU  - Kernan NA
FAU - O'Reilly, R J
AU  - O'Reilly RJ
LA  - eng
GR  - CA23766/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Immunoglobulins)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Pokeweed Mitogens)
RN  - 0 (Staphylococcal Protein A)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Bone Marrow Transplantation
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Granulocytes/immunology
MH  - Hematopoietic Stem Cells/classification/*immunology
MH  - Humans
MH  - Immunoglobulins/biosynthesis
MH  - Infections/etiology/immunology
MH  - Killer Cells, Natural/immunology
MH  - Leukemia/immunology/surgery
MH  - Lymphocyte Activation
MH  - *Lymphocyte Depletion
MH  - Lymphocytes/classification/immunology
MH  - Male
MH  - Middle Aged
MH  - Phytohemagglutinins
MH  - Pokeweed Mitogens
MH  - Postoperative Complications/etiology/mortality
MH  - Postoperative Period
MH  - Staphylococcal Protein A
MH  - *T-Lymphocytes/classification/immunology
EDAT- 1989/04/01 00:00
MHDA- 1989/04/01 00:01
CRDT- 1989/04/01 00:00
PHST- 1989/04/01 00:00 [pubmed]
PHST- 1989/04/01 00:01 [medline]
PHST- 1989/04/01 00:00 [entrez]
AID - S0006-4971(20)75563-1 [pii]
PST - ppublish
SO  - Blood. 1989 Apr;73(5):1340-50.