PMID- 26492067
OWN - NLM
STAT- MEDLINE
DCOM- 20160408
LR  - 20240109
IS  - 0231-5882 (Print)
IS  - 0231-5882 (Linking)
VI  - 35
IP  - 1
DP  - 2016 Jan
TI  - The cytoprotective effects of ethanol extract of Ecklonia cava against oxidative 
      stress are associated with upregulation of Nrf2-mediated HO-1 and NQO-1 
      expression through activation of the MAPK pathway.
PG  - 45-53
LID - 10.4149/gpb_2015029 [doi]
AB  - The aim of the present study was to examine the cytoprotective effect of Ecklonia 
      cava against oxidative stress in C2C12 myoblasts. The ethanol extract of E. cava 
      (EEEC) prevented hydrogen peroxide (H(2)O(2))-induced inhibition of the growth of 
      C2C12 myoblasts and exhibited scavenging activity against intracellular reactive 
      oxygen species (ROS) induced by H(2)O(2). EEEC treatment attenuated H2O2-induced 
      comet tail formation and phospho-histone gammaH2A.X expression. Furthermore, EEEC 
      treatment enhanced the level of the phosphorylated form of nuclear factor 
      erythroid 2- related factor 2 (Nrf2) and its nuclear translocation, which was 
      associated with the induction of heme oxygenase-1 (HO-1) and NADPH-quinone 
      oxidoreductase 1 (NQO-1). Zinc protoporphyrin IX, a HO-1 competitive inhibitor, 
      significantly abolished the protective effects of EEEC against H(2)O(2)-induced ROS 
      generation and growth inhibition in C2C12 myoblasts. Transient transfection with 
      Nrf2-specific small interfering RNA restored the elevated HO-1 and NQO-1 
      expression and the phosphorylation of Nrf2 to near normal levels. The EEEC 
      treatment also induced the activation of mitogen-activated protein kinases 
      (MAPKs), and specific inhibitors of MAPKs abolished upregulated HO-1 and NQO-1, 
      as well as the phosphorylation of Nrf2. Taken together, these data suggest that 
      EEEC attenuates oxidative stress by activating Nrf2-mediated HO-1 and inducing 
      NQO-1 via the activation of MAPK signaling pathways.
FAU - Choi, Yung Hyun
AU  - Choi YH
AD  - Department of Biochemistry, Dongeui University College of Korean Medicine, 52-57, 
      Yangjeong-ro, Busanjin, Busan 614-052, Republic of Korea. choiyh@deu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151022
PL  - Slovakia
TA  - Gen Physiol Biophys
JT  - General physiology and biophysics
JID - 8400604
RN  - 0 (Cell Extracts)
RN  - 0 (Membrane Proteins)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Reactive Oxygen Species)
RN  - 3K9958V90M (Ethanol)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.14.18 (Hmox1 protein, mouse)
RN  - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
RN  - EC 1.6.5.2 (Nqo1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Cell Extracts/administration & dosage/chemistry
MH  - Cell Line
MH  - Cell Proliferation/drug effects/physiology
MH  - Cell Survival/drug effects/physiology
MH  - Cytoprotection/drug effects/physiology
MH  - Dose-Response Relationship, Drug
MH  - Ethanol/chemistry
MH  - Heme Oxygenase-1/*metabolism
MH  - MAP Kinase Signaling System/drug effects/*physiology
MH  - Membrane Proteins/*metabolism
MH  - Mice
MH  - Myoblasts/drug effects/metabolism
MH  - NAD(P)H Dehydrogenase (Quinone)/*metabolism
MH  - NF-E2-Related Factor 2/*metabolism
MH  - Oxidative Stress/drug effects/*physiology
MH  - Phaeophyceae/*chemistry
MH  - Reactive Oxygen Species/metabolism
MH  - Up-Regulation/physiology/radiation effects
EDAT- 2015/10/23 06:00
MHDA- 2016/04/09 06:00
CRDT- 2015/10/23 06:00
PHST- 2015/04/01 00:00 [received]
PHST- 2015/06/23 00:00 [accepted]
PHST- 2015/10/23 06:00 [entrez]
PHST- 2015/10/23 06:00 [pubmed]
PHST- 2016/04/09 06:00 [medline]
AID - 10.4149/gpb_2015029 [doi]
PST - ppublish
SO  - Gen Physiol Biophys. 2016 Jan;35(1):45-53. doi: 10.4149/gpb_2015029. Epub 2015 
      Oct 22.