PMID- 26492318
OWN - NLM
STAT- MEDLINE
DCOM- 20161018
LR  - 20181113
IS  - 1559-2324 (Electronic)
IS  - 1559-2316 (Print)
IS  - 1559-2316 (Linking)
VI  - 10
IP  - 12
DP  - 2015
TI  - Peanut witches' broom (PnWB) phytoplasma-mediated leafy flower symptoms and 
      abnormal vascular bundles development.
PG  - e1107690
LID - 10.1080/15592324.2015.1107690 [doi]
LID - e1107690
AB  - The peanut witches' broom (PnWB) phytoplasma causes virescence symptoms such as 
      phyllody (leafy flower) in infected peanuts. However, the obligate nature of 
      phytoplasma limits the study of host-pathogen interactions, and the detailed 
      anatomy of PnWB-infected plants has yet to be reported. Here, we demonstrate that 
      4',6'-diamidino-2-phenylindole (DAPI) staining can be used to track PnWB 
      infection. The DAPI-stained phytoplasma cells were observed in phloem/internal 
      phloem tissues, and changes in vascular bundle morphology, including increasing 
      pith rays and thinner cell walls in the xylem, were found. We also discerned the 
      cell types comprising PnWB in infected sieve tube members. These results suggest 
      that the presence of PnWB in phloem tissue facilitates the transmission of 
      phytoplasma via sap-feeding insect vectors. In addition, PnWB in sieve tube 
      members and changes in vascular bundle morphology might strongly promote the 
      ability of phytoplasmas to assimilate nutrients. These data will help further an 
      understanding of the obligate life cycle and host-pathogen interactions of 
      phytoplasma.
FAU - Liu, Chi-Te
AU  - Liu CT
AD  - a Institue of Biotechnology; National Taiwan University ; Taipei , Taiwan.
AD  - b Agricultural Biotechnology Research Center; Academia Sinica ; Taipei , Taiwan.
FAU - Huang, Hsin-Mei
AU  - Huang HM
AD  - a Institue of Biotechnology; National Taiwan University ; Taipei , Taiwan.
FAU - Hong, Syuan-Fei
AU  - Hong SF
AD  - a Institue of Biotechnology; National Taiwan University ; Taipei , Taiwan.
FAU - Kuo-Huang, Ling-Long
AU  - Kuo-Huang LL
AD  - d Department of Life Science ; National Taiwan University ; Taipei , Taiwan.
FAU - Yang, Chiao-Yin
AU  - Yang CY
AD  - e Department of Plant Pathology and Microbiology ; National Taiwan University ; 
      Taipei , Taiwan.
FAU - Lin, Yen-Yu
AU  - Lin YY
AD  - e Department of Plant Pathology and Microbiology ; National Taiwan University ; 
      Taipei , Taiwan.
FAU - Lin, Chan-Pin
AU  - Lin CP
AD  - a Institue of Biotechnology; National Taiwan University ; Taipei , Taiwan.
AD  - e Department of Plant Pathology and Microbiology ; National Taiwan University ; 
      Taipei , Taiwan.
FAU - Lin, Shih-Shun
AU  - Lin SS
AD  - a Institue of Biotechnology; National Taiwan University ; Taipei , Taiwan.
AD  - b Agricultural Biotechnology Research Center; Academia Sinica ; Taipei , Taiwan.
AD  - c Center of Biotechnology; National Taiwan University ; Taipei , Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Plant Signal Behav
JT  - Plant signaling & behavior
JID - 101291431
SB  - IM
MH  - Arachis/*microbiology
MH  - Catharanthus/microbiology
MH  - Flowers/*microbiology
MH  - Microscopy, Confocal
MH  - Phytoplasma/*physiology
MH  - Plant Diseases/*microbiology
MH  - Plant Leaves/*microbiology
MH  - Plant Stems/*microbiology
MH  - Plant Vascular Bundle/*growth & development/microbiology
PMC - PMC4854342
OTO - NOTNLM
OT  - 4',6'-diamidino-2-phenylindole staining
OT  - broom phytoplasma
OT  - internal phloem
OT  - leafy flower
OT  - peanut witches'
OT  - vascular bundle
EDAT- 2015/10/23 06:00
MHDA- 2016/10/19 06:00
PMCR- 2015/10/22
CRDT- 2015/10/23 06:00
PHST- 2015/10/23 06:00 [entrez]
PHST- 2015/10/23 06:00 [pubmed]
PHST- 2016/10/19 06:00 [medline]
PHST- 2015/10/22 00:00 [pmc-release]
AID - 1107690 [pii]
AID - 10.1080/15592324.2015.1107690 [doi]
PST - ppublish
SO  - Plant Signal Behav. 2015;10(12):e1107690. doi: 10.1080/15592324.2015.1107690.