PMID- 2649289
OWN - NLM
STAT- MEDLINE
DCOM- 19890519
LR  - 20181113
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 75
IP  - 2
DP  - 1989 Feb
TI  - Quantitative analysis of cultured thymic reticulo-epithelial cells labelled by 
      different antibodies: a flow cytometric study.
PG  - 292-6
AB  - Quantitative measurements of cultured human and murine thymic, and human thymoma 
      reticuloepithelial cells (REC), immunolabeled by different antibodies (Ab) (TE3, 
      TE4, anti-HTLV p19(p19), lu5, K11 and Aks) and by thymic hormones (thymulin and 
      thymosin alpha 1 (Ta1)) within these cells, were performed using a flow 
      cytometric technique. The anti-keratin polyclonal Ab labeled nearly the whole 
      human or murine population. The p19 monoclonal Ab (MoAb), specific for the 
      subcortical/medullary thymic regions, labelled 37-77% of the human REC. The TE3 
      MoAb, specific for the cortical region, labelled 54-83% of the REC. These 
      percentages suggest that the cultured thymic REC (TREC) had markers of both 
      regions together and therefore that these markers are not absolutely specific to 
      determine their subcortical/medullary or cortical thymic origin. For the three 
      populations there were more cells containing Ta1 than thymulin. The overlap of 
      the percentage of labelled cells suggests that the same cell could synthesize the 
      two hormones and that these hormones could be localized within the TE3 positive 
      cells.
FAU - Fabien, N
AU  - Fabien N
AD  - Laboratoire d'Immunologie U.F.R.A. Carrel, Lyon, France.
FAU - Auger, C
AU  - Auger C
FAU - Bonnard, M
AU  - Bonnard M
FAU - Andreoni, C
AU  - Andreoni C
FAU - Rigal, D
AU  - Rigal D
FAU - Monier, J C
AU  - Monier JC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Antibodies)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology
MH  - Cells, Cultured
MH  - Child
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mononuclear Phagocyte System/*immunology
MH  - Thymoma/*immunology
MH  - Thymus Gland/*immunology
MH  - Thymus Neoplasms/*immunology
PMC - PMC1542116
EDAT- 1989/02/01 00:00
MHDA- 1989/02/01 00:01
PMCR- 1990/02/01
CRDT- 1989/02/01 00:00
PHST- 1989/02/01 00:00 [pubmed]
PHST- 1989/02/01 00:01 [medline]
PHST- 1989/02/01 00:00 [entrez]
PHST- 1990/02/01 00:00 [pmc-release]
PST - ppublish
SO  - Clin Exp Immunol. 1989 Feb;75(2):292-6.