PMID- 26497118
OWN - NLM
STAT- MEDLINE
DCOM- 20161006
LR  - 20171116
IS  - 1098-1136 (Electronic)
IS  - 0894-1491 (Linking)
VI  - 64
IP  - 3
DP  - 2016 Mar
TI  - Fibroblast-derived tenascin-C promotes Schwann cell migration through beta1-integrin 
      dependent pathway during peripheral nerve regeneration.
PG  - 374-85
LID - 10.1002/glia.22934 [doi]
AB  - Peripheral nerve regeneration requires precise coordination and dynamic 
      interaction among various types of cells in the tissue. It remains unclear, 
      however, whether the cellular crosstalk between fibroblasts and Schwann cells 
      (SCs) is related to phenotype modulation of SCs, a critical cellular process 
      after peripheral nerve injury. In this study, microarray analysis revealed that a 
      total of 6,046 genes were differentially expressed in the proximal nerve segment 
      after sciatic nerve transection in rats, and bioinformatics analysis further 
      identified tenascin-C (TNC), an extracellular matrix (ECM) protein, as a key gene 
      regulator. TNC was abundantly produced by nerve fibroblasts accumulating at the 
      lesion site, rather than by SCs as usually expected. TNC significantly promoted 
      SC migration without effects on SC proliferation in primary culture. In 
      co-culture of fibroblasts and SCs, inhibition of TNC expression either by siRNA 
      transfection or antibody blockade could suppress SC migration, while 
      TNC-stimulated SC migration was mediated by TNC binding to beta1-integrin receptor 
      in SCs through activation of Rac1 effectors. The in vivo evidence showed that 
      exogenous TNC protein enhanced SC migration and axonal regrowth. Our results 
      highlight that TNC-mediated cellular interaction between fibroblasts and SCs may 
      regulate SC migration through beta1-integrin-dependent pathway during peripheral 
      nerve regeneration.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Zhang, Zhanhu
AU  - Zhang Z
AD  - Model Animal Research Center, Nanjing University, Nanjing, China.
AD  - Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of 
      Neuroregeneration, Nantong University, Nantong, China.
FAU - Yu, Bin
AU  - Yu B
AD  - Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of 
      Neuroregeneration, Nantong University, Nantong, China.
FAU - Gu, Yun
AU  - Gu Y
AD  - Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of 
      Neuroregeneration, Nantong University, Nantong, China.
FAU - Zhou, Songlin
AU  - Zhou S
AD  - Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of 
      Neuroregeneration, Nantong University, Nantong, China.
FAU - Qian, Tianmei
AU  - Qian T
AD  - Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of 
      Neuroregeneration, Nantong University, Nantong, China.
FAU - Wang, Yongjun
AU  - Wang Y
AD  - Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of 
      Neuroregeneration, Nantong University, Nantong, China.
FAU - Ding, Guohui
AU  - Ding G
AD  - Key Lab of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese 
      Academy of Sciences, Shanghai, China.
FAU - Ding, Fei
AU  - Ding F
AD  - Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of 
      Neuroregeneration, Nantong University, Nantong, China.
FAU - Gu, Xiaosong
AU  - Gu X
AD  - Model Animal Research Center, Nanjing University, Nanjing, China.
AD  - Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of 
      Neuroregeneration, Nantong University, Nantong, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151024
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Integrin beta1)
RN  - 0 (S100 Calcium Binding Protein beta Subunit)
RN  - 0 (Tenascin)
RN  - EC 1.14.11.2 (Procollagen-Proline Dioxygenase)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
RN  - EC 5.3.4.1 (P4hb protein, rat)
RN  - EC 5.3.4.1 (Protein Disulfide-Isomerases)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/metabolism
MH  - Cell Movement/*drug effects
MH  - Cell Proliferation/drug effects/genetics
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Disease Models, Animal
MH  - Fibroblasts/physiology
MH  - Gene Regulatory Networks
MH  - Integrin beta1/*metabolism
MH  - Male
MH  - Nerve Regeneration/*physiology
MH  - Procollagen-Proline Dioxygenase/metabolism
MH  - Protein Disulfide-Isomerases/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - S100 Calcium Binding Protein beta Subunit/metabolism
MH  - Schwann Cells/*drug effects
MH  - Sciatic Neuropathy/*pathology/physiopathology
MH  - Signal Transduction/*drug effects/genetics/physiology
MH  - Tenascin/metabolism/*pharmacology
MH  - Time Factors
MH  - Wound Healing/drug effects/genetics
MH  - rho GTP-Binding Proteins/metabolism
OTO - NOTNLM
OT  - Schwann cells
OT  - cell migration
OT  - crosstalk
OT  - fibroblasts
OT  - sciatic nerve injury
OT  - tenascin-C
EDAT- 2015/10/27 06:00
MHDA- 2016/10/08 06:00
CRDT- 2015/10/27 06:00
PHST- 2014/10/14 00:00 [received]
PHST- 2015/09/28 00:00 [accepted]
PHST- 2015/10/27 06:00 [entrez]
PHST- 2015/10/27 06:00 [pubmed]
PHST- 2016/10/08 06:00 [medline]
AID - 10.1002/glia.22934 [doi]
PST - ppublish
SO  - Glia. 2016 Mar;64(3):374-85. doi: 10.1002/glia.22934. Epub 2015 Oct 24.