PMID- 26497177
OWN - NLM
STAT- MEDLINE
DCOM- 20160517
LR  - 20170225
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 161
IP  - 1
DP  - 2016 Jan
TI  - Productive HIV-1 infection is enriched in CD4(-)CD8(-) double negative (DN) T 
      cells at pleural sites of dual infection with HIV and Mycobacterium tuberculosis.
PG  - 181-7
LID - 10.1007/s00705-015-2640-7 [doi]
AB  - A higher human immunodeficiency virus 1 (HIV-1) viral load at pleural sites 
      infected with Mycobacterium tuberculosis (MTB) than in peripheral blood has been 
      documented. However, the cellular source of productive HIV infection in 
      HIV-1/MTB-coinfected pleural fluid mononuclear cells (PFMCs) remains unclear. In 
      this study, we observed significant quantities of HIV-1 p24(+) lymphocytes in 
      PFMCs, but not in peripheral blood mononuclear cells (PBMCs). HIV-1 p24(+) 
      lymphocytes were mostly enriched in DN T cells. Intracellular CD4 expression was 
      detectable in HIV-1 p24(+) DN T cells. HIV-1 p24(+) DN T cells showed lower 
      surface expression of human leukocyte antigen (HLA)-ABC and tetherin than did 
      HIV-1 p24(+) CD4 T cells. Upon in vitro infection of PFMC CD4 T cells from TB 
      mono-infected subjects, Nef- and/or Vpu-deleted HIV mutants showed lower 
      generation of HIV-1 p24(+) DN T cells than the wild-type virus. These data 
      indicate that productively HIV-1-infected DN T cells, generated through 
      down-modulation of surface CD4, likely by HIV-1 Nef and Vpu, are the predominant 
      source of HIV-1 at pleural sites of HIV/MTB coinfection.
FAU - Meng, Qinglai
AU  - Meng Q
AD  - Division of Infectious Diseases, Department of Medicine, BRB 10W, Case Western 
      Reserve University, 10900 Euclid Ave, Cleveland, OH, 44106-4984, USA. 
      qxm8@case.edu.
FAU - Canaday, David H
AU  - Canaday DH
AD  - Division of Infectious Diseases, Department of Medicine, BRB 10W, Case Western 
      Reserve University, 10900 Euclid Ave, Cleveland, OH, 44106-4984, USA.
AD  - Veterans Affairs Medical Center, Cleveland, OH, USA.
FAU - McDonald, David J
AU  - McDonald DJ
AD  - Department of Molecular Biology and Microbiology, Case Western Reserve 
      University, Cleveland, OH, USA.
FAU - Mayanja-Kizza, Harriet
AU  - Mayanja-Kizza H
AD  - Division of Infectious Diseases, Department of Medicine, BRB 10W, Case Western 
      Reserve University, 10900 Euclid Ave, Cleveland, OH, 44106-4984, USA.
AD  - Makerere University, Kampala, Uganda.
FAU - Baseke, Joy
AU  - Baseke J
AD  - Joint Clinical Research Center, Kampala, Uganda.
FAU - Toossi, Zahra
AU  - Toossi Z
AUID- ORCID: 0000-0002-2060-0674
AD  - Division of Infectious Diseases, Department of Medicine, BRB 10W, Case Western 
      Reserve University, 10900 Euclid Ave, Cleveland, OH, 44106-4984, USA. 
      zxt2@case.edu.
AD  - Veterans Affairs Medical Center, Cleveland, OH, USA. zxt2@case.edu.
LA  - eng
GR  - AI-087511/AI/NIAID NIH HHS/United States
GR  - P30 AI-036219/AI/NIAID NIH HHS/United States
GR  - AI-70022/AI/NIAID NIH HHS/United States
GR  - HL-051636/HL/NHLBI NIH HHS/United States
GR  - AI-080313/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20151023
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
SB  - IM
MH  - Adult
MH  - Coinfection/*immunology/microbiology/virology
MH  - Female
MH  - HIV Infections/*immunology/microbiology/virology
MH  - HIV-1/*physiology
MH  - Humans
MH  - Male
MH  - Mycobacterium tuberculosis/*physiology
MH  - Pleura/*immunology
MH  - T-Lymphocytes/*immunology
MH  - Tuberculosis/*immunology/microbiology
MH  - Young Adult
OTO - NOTNLM
OT  - Double-negative T cell
OT  - HIV-1
OT  - PFMC
OT  - T cell
OT  - Tuberculosis
EDAT- 2015/10/27 06:00
MHDA- 2016/05/18 06:00
CRDT- 2015/10/27 06:00
PHST- 2015/07/12 00:00 [received]
PHST- 2015/10/06 00:00 [accepted]
PHST- 2015/10/27 06:00 [entrez]
PHST- 2015/10/27 06:00 [pubmed]
PHST- 2016/05/18 06:00 [medline]
AID - 10.1007/s00705-015-2640-7 [pii]
AID - 10.1007/s00705-015-2640-7 [doi]
PST - ppublish
SO  - Arch Virol. 2016 Jan;161(1):181-7. doi: 10.1007/s00705-015-2640-7. Epub 2015 Oct 
      23.