PMID- 26500069
OWN - NLM
STAT- MEDLINE
DCOM- 20160912
LR  - 20151120
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 12
IP  - 6
DP  - 2015 Dec
TI  - Dynamic expression of miR-206-3p during mouse skin development is independent of 
      keratinocyte differentiation.
PG  - 8113-20
LID - 10.3892/mmr.2015.4456 [doi]
AB  - MicroRNA-206 (miR-206), the homolog of which in mice is termed miR-206-3p, is a 
      muscle-specific miRNA known to be important in the development of skeletal 
      muscle, and is involved in smooth muscle innervation of the airway through the 
      post‑transcriptional suppression of brain‑derived neurotrophic factor (Bdnf). 
      miR‑206‑3p is also expressed at significant levels in adult and embryonic skin; 
      however, its functional roles in adult skin and during skin development remain to 
      be fully elucidated. In the present study, the spatiotemporal expression of 
      miR‑206‑3p and its target‑gene, Bdnf, during mouse skin development were 
      investigated. The expression level of miR‑206‑3p increased from 13.5 days 
      postcoitus (dpc), peaked at 17.5 dpc and declined following birth. The observed 
      temporal profile of the expression of miR‑206‑3p was accompanied by an inverse 
      change in the protein expression levels of BDNF. However, the mRNA expression 
      levels of Bdnf did not parallel those of BDNF protein. The localization of the 
      expression of miR‑206‑3p was similar, or located near that of ubiquitin 
      carboxyl‑terminal hydrolase L1 during skin development. An in vitro keratinocyte 
      model demonstrated no significant differences between primary and differentiated 
      keratinocytes in the expression levels of either miR‑206‑3p (P=0.227) or Bdnf 
      (mRNA, P=0.118; mature BDNF, P=0.106; pro‑BDNF, P=0.905). These findings indicate 
      a potential role for miR‑206‑3p in cutaneous innervation, which largely relies on 
      BDNF neurotrophic support and is independent of keratinocyte differentiation. The 
      results of the present study suggested that this novel mechanism may be targeted 
      for developing potential therapeutic approaches.
FAU - Mu, Yuan
AU  - Mu Y
AD  - Department of Clinical Laboratory, Nanjing Children's Hospital, Nanjing Medical 
      University, Nanjing, Jiangsu 210008, P.R. China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - Department of Clinical Laboratory and Hematology, School of Medicine, Jiangsu 
      University, Zhenjiang, Jiangsu 212013, P.R. China.
FAU - Wu, Wei-Jiang
AU  - Wu WJ
AD  - Department of Histology and Embryology, School of Medicine, Jiangsu University, 
      Zhenjiang, Jiangsu 212013, P.R. China.
FAU - Hu, Li-Chao
AU  - Hu LC
AD  - Department of Clinical Laboratory and Hematology, School of Medicine, Jiangsu 
      University, Zhenjiang, Jiangsu 212013, P.R. China.
FAU - Chen, Hong-Bing
AU  - Chen HB
AD  - Department of Clinical Laboratory, Nanjing Children's Hospital, Nanjing Medical 
      University, Nanjing, Jiangsu 210008, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151020
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn206 microRNA, mouse)
RN  - 0 (Protein Precursors)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Female
MH  - Keratinocytes/cytology/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/genetics/*metabolism
MH  - Protein Precursors/genetics/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Skin/*metabolism
EDAT- 2015/10/27 06:00
MHDA- 2016/09/13 06:00
CRDT- 2015/10/27 06:00
PHST- 2014/12/24 00:00 [received]
PHST- 2015/09/16 00:00 [accepted]
PHST- 2015/10/27 06:00 [entrez]
PHST- 2015/10/27 06:00 [pubmed]
PHST- 2016/09/13 06:00 [medline]
AID - 10.3892/mmr.2015.4456 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 Dec;12(6):8113-20. doi: 10.3892/mmr.2015.4456. Epub 2015 Oct 
      20.