PMID- 26500490
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151027
LR  - 20201001
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 9
DP  - 2015
TI  - Dendritic morphology, synaptic transmission, and activity of mature granule cells 
      born following pilocarpine-induced status epilepticus in the rat.
PG  - 384
LID - 10.3389/fncel.2015.00384 [doi]
LID - 384
AB  - To understand the potential role of enhanced hippocampal neurogenesis after 
      pilocarpine-induced status epilepticus (SE) in the development of epilepsy, we 
      quantitatively analyzed the geometry of apical dendrites, synaptic transmission, 
      and activation levels of normotopically distributed mature newborn granule cells 
      in the rat. SE in male Sprague-Dawley rats (between 6 and 7 weeks old) lasting 
      for more than 2 h was induced by an intraperitoneal injection of pilocarpine. The 
      complexity, spine density, miniature post-synaptic currents, and 
      activity-regulated cytoskeleton-associated protein (Arc) expression of granule 
      cells born 5 days after SE were studied between 10 and 17 weeks after CAG-GFP 
      retroviral vector-mediated labeling. Mature granule cells born after SE had 
      dendritic complexity similar to that of granule cells born naturally, but with 
      denser mushroom-like spines in dendritic segments located in the outer molecular 
      layer. Miniature inhibitory post-synaptic currents (mIPSCs) were similar between 
      the controls and rats subjected to SE; however, smaller miniature excitatory 
      post-synaptic current (mEPSC) amplitude with a trend toward less frequent was 
      found in mature granule cells born after SE. After maturation, granule cells born 
      after SE did not show denser Arc expression in the resting condition or 2 h after 
      being activated by pentylenetetrazol-induced transient seizure activity than 
      vicinal GFP-unlabeled granule cells. Thus our results suggest that normotopic 
      granule cells born after pilocarpine-induced SE are no more active when mature 
      than age-matched, naturally born granule cells.
FAU - Gao, Fei
AU  - Gao F
AD  - Department of Physiology, Shandong University School of Medicine Jinan, China.
FAU - Song, Xueying
AU  - Song X
AD  - Department of Physiology, Shandong University School of Medicine Jinan, China.
FAU - Zhu, Dexiao
AU  - Zhu D
AD  - Department of Physiology, Shandong University School of Medicine Jinan, China.
FAU - Wang, Xiaochen
AU  - Wang X
AD  - Department of Physiology, Shandong University School of Medicine Jinan, China.
FAU - Hao, Aijun
AU  - Hao A
AD  - Department of Histology and Embryology, Shandong University School of Medicine 
      Jinan, China.
FAU - Nadler, J Victor
AU  - Nadler JV
AD  - Departments of Pharmacology and Neurobiology, Duke University Medical Center 
      Durham, NC, USA.
FAU - Zhan, Ren-Zhi
AU  - Zhan RZ
AD  - Department of Physiology, Shandong University School of Medicine Jinan, China.
LA  - eng
PT  - Journal Article
DEP - 20151007
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC4596052
OTO - NOTNLM
OT  - Sholl analysis
OT  - activity-regulated cytoskeleton-associated protein
OT  - dendritic spine
OT  - epilepsy
OT  - neurogenesis
OT  - retroviral vector
EDAT- 2015/10/27 06:00
MHDA- 2015/10/27 06:01
PMCR- 2015/01/01
CRDT- 2015/10/27 06:00
PHST- 2015/03/07 00:00 [received]
PHST- 2015/09/14 00:00 [accepted]
PHST- 2015/10/27 06:00 [entrez]
PHST- 2015/10/27 06:00 [pubmed]
PHST- 2015/10/27 06:01 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2015.00384 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2015 Oct 7;9:384. doi: 10.3389/fncel.2015.00384. eCollection 
      2015.