PMID- 26502094
OWN - NLM
STAT- MEDLINE
DCOM- 20160603
LR  - 20220129
IS  - 1423-0259 (Electronic)
IS  - 0030-3747 (Linking)
VI  - 54
IP  - 4
DP  - 2015
TI  - Complement Stimulates Retinal Pigment Epithelial Cells to Undergo 
      Pro-Inflammatory Changes.
PG  - 195-203
LID - 10.1159/000439596 [doi]
AB  - BACKGROUND/AIMS: We examined the effect of human complement sera (HCS) on retinal 
      pigment epithelial (RPE) cells with respect to pro-inflammatory mediators 
      relevant in early age-related macular degeneration (AMD). METHODS: RPE cells were 
      treated with complement-containing HCS or with heat-inactivated (HI) HCS or 
      C7-deficient HCS as controls. Cells were analysed for C5b-9 using 
      immunocytochemistry and flow cytometry. Interleukin (IL)-6, IL-8, and monocyte 
      chemoattractant protein-1 (MCP-1) were quantified by ELISA and RT-PCR. Tumour 
      necrosis factor-alpha (TNF-alpha), intercellular adhesion molecule-1 (ICAM-1) and 
      vascular cell adhesion molecule-1 (VCAM-1), were analysed by Western blotting. 
      The intracellular distribution of nuclear factor (NF)-x03BA;B was investigated by 
      immunofluorescence. RESULTS: A concentration-dependent increased staining for 
      C5b-9 but no influence on cell viability was observed after HCS treatment. ELISA 
      and RT-PCR analysis revealed elevated secretion and expression of IL-6, IL-8, and 
      MCP-1. Western blot analysis showed a concentration-dependent increase in ICAM-1, 
      VCAM-1, and TNF-alpha in response to HCS, and immunofluorescence staining revealed 
      nuclear translocation of NF-x03BA;B. CONCLUSION: This study suggests that 
      complement stimulates NF-x03BA;B activation in RPE cells that might further 
      create a pro-inflammatory environment. All these factors together may support 
      early AMD development.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - Lueck, Katharina
AU  - Lueck K
AD  - Ophtha-Lab, Department of Ophthalmology at St. Franziskus Hospital, Muenster, 
      Germany.
FAU - Busch, Martin
AU  - Busch M
FAU - Moss, Stephen E
AU  - Moss SE
FAU - Greenwood, John
AU  - Greenwood J
FAU - Kasper, Maren
AU  - Kasper M
FAU - Lommatzsch, Albrecht
AU  - Lommatzsch A
FAU - Pauleikhoff, Daniel
AU  - Pauleikhoff D
FAU - Wasmuth, Susanne
AU  - Wasmuth S
LA  - eng
GR  - MR/M02282X/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151027
PL  - Switzerland
TA  - Ophthalmic Res
JT  - Ophthalmic research
JID - 0267442
RN  - 0 (Complement Membrane Attack Complex)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (NF-kappa B)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 9007-36-7 (Complement System Proteins)
SB  - IM
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Complement Membrane Attack Complex/*metabolism
MH  - Complement System Proteins/*physiology
MH  - Cytokines/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - Inflammation Mediators/*metabolism
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Macular Degeneration/metabolism
MH  - NF-kappa B/*metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Retinal Pigment Epithelium/*metabolism
MH  - Vascular Cell Adhesion Molecule-1/metabolism
EDAT- 2015/10/27 06:00
MHDA- 2016/06/04 06:00
CRDT- 2015/10/27 06:00
PHST- 2015/03/26 00:00 [received]
PHST- 2015/08/21 00:00 [accepted]
PHST- 2015/10/27 06:00 [entrez]
PHST- 2015/10/27 06:00 [pubmed]
PHST- 2016/06/04 06:00 [medline]
AID - 000439596 [pii]
AID - 10.1159/000439596 [doi]
PST - ppublish
SO  - Ophthalmic Res. 2015;54(4):195-203. doi: 10.1159/000439596. Epub 2015 Oct 27.