PMID- 26502296
OWN - NLM
STAT- MEDLINE
DCOM- 20160701
LR  - 20191210
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 140
IP  - 23
DP  - 2015 Dec 7
TI  - Direct detection of illicit drugs from biological fluids by desorption/ionization 
      mass spectrometry with nanoporous silicon microparticles.
PG  - 7926-33
LID - 10.1039/c5an01754h [doi]
AB  - Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is a 
      high throughput analytical technique capable of detecting low molecular weight 
      analytes, including illicit drugs, and with potential applications in forensic 
      toxicology as well as athlete and workplace testing, particularly for biological 
      fluids (oral fluids, urine and blood). However, successful detection of illicit 
      drugs using SALDI-MS often requires extraction steps to reduce the inherent 
      complexity of biological fluids. Here, we demonstrate an all-in-one extraction 
      and analytical system consisting of hydrophobically functionalized porous silicon 
      microparticles (pSi-MPs) for affinity SALDI-MS of prescription and illicit drugs. 
      This novel approach allows for the analysis of drugs from multiple biological 
      fluids without sample preparation protocols. The effect of pSi-MP size, pore 
      diameter, pore depth and functionalization on analytical performance is 
      investigated. pSi-MPs were optimized for the rapid and high sensitivity detection 
      of methadone, cocaine and 3,4-methylenedioxymethamphetamine (MDMA). This 
      optimized system allowed extraction and detection of methadone from spiked saliva 
      and clinical urine samples. Furthermore, by detecting oxycodone in additional 
      clinical saliva and plasma samples, we were able to demonstrate the versatility 
      of the pSi-MP SALDI-MS technique.
FAU - Guinan, T M
AU  - Guinan TM
AD  - ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson 
      Institute, Australia. nico.voelcker@unisa.edu.au.
FAU - Kirkbride, P
AU  - Kirkbride P
FAU - Della Vedova, C B
AU  - Della Vedova CB
FAU - Kershaw, S G
AU  - Kershaw SG
FAU - Kobus, H
AU  - Kobus H
FAU - Voelcker, N H
AU  - Voelcker NH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151026
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Illicit Drugs)
RN  - Z4152N8IUI (Silicon)
SB  - IM
MH  - Body Fluids/*chemistry
MH  - Chemistry Techniques, Analytical/*methods
MH  - Humans
MH  - Illicit Drugs/*analysis/blood
MH  - Nanoparticles/*chemistry
MH  - Porosity
MH  - Silicon/*chemistry
MH  - *Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 2015/10/27 06:00
MHDA- 2016/07/02 06:00
CRDT- 2015/10/27 06:00
PHST- 2015/10/27 06:00 [entrez]
PHST- 2015/10/27 06:00 [pubmed]
PHST- 2016/07/02 06:00 [medline]
AID - 10.1039/c5an01754h [doi]
PST - ppublish
SO  - Analyst. 2015 Dec 7;140(23):7926-33. doi: 10.1039/c5an01754h. Epub 2015 Oct 26.