PMID- 26503469
OWN - NLM
STAT- MEDLINE
DCOM- 20160922
LR  - 20181202
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 6
IP  - 38
DP  - 2015 Dec 1
TI  - Amphiregulin enhances intercellular adhesion molecule-1 expression and promotes 
      tumor metastasis in human osteosarcoma.
PG  - 40880-95
LID - 10.18632/oncotarget.5679 [doi]
AB  - Osteosarcoma is a common, high malignant, and metastatic bone cancer. 
      Amphiregulin (AREG) has been associated with cancer cellular activities. However, 
      the effect of AREG on metastasis activity in human osteosarcoma cells has yet to 
      be determined. We determined that AREG increases the expression of intercellular 
      adhesion molecule-1 (ICAM-1) through PI3K/Akt signaling pathway via its 
      interaction with the epidermal growth factor receptor, thus resulting in the 
      enhanced cell migration of osteosarcoma. Furthermore, AREG stimulation increased 
      the association of NF-kappaB to ICAM-1 promoter which then up-regulated ICAM-1 
      expression. Finally, we observed that shRNA silencing of AREG decreased 
      osteosarcoma metastasis in vivo. Our findings revealed a relationship between 
      osteosarcoma metastatic potential and AREG expression and the modulating effect 
      of AREG on ICAM-1 expression.
FAU - Liu, Ju-Fang
AU  - Liu JF
AD  - Central Laboratory, Shin-Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
FAU - Tsao, Ya-Ting
AU  - Tsao YT
AD  - Department of Orthopedic Surgery, National Taiwan University Hospital, Taipei, 
      Taiwan.
FAU - Hou, Chun-Han
AU  - Hou CH
AD  - Department of Orthopedic Surgery, National Taiwan University Hospital, Taipei, 
      Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (ICAM1 protein, human)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Amphiregulin
MH  - Animals
MH  - Apoptosis
MH  - Blotting, Western
MH  - Bone Neoplasms/genetics/metabolism/*pathology
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Chromatin Immunoprecipitation
MH  - EGF Family of Proteins/genetics/*metabolism
MH  - ErbB Receptors/genetics/metabolism
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Intercellular Adhesion Molecule-1/chemistry/genetics/*metabolism
MH  - Lung Neoplasms/genetics/metabolism/*secondary
MH  - Male
MH  - Mice
MH  - Mice, SCID
MH  - NF-kappa B/genetics/metabolism
MH  - Osteosarcoma/genetics/metabolism/*pathology
MH  - Phosphatidylinositol 3-Kinases/genetics/metabolism
MH  - RNA, Messenger/genetics
MH  - RNA, Small Interfering/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Tumor Cells, Cultured
MH  - Xenograft Model Antitumor Assays
PMC - PMC4747375
OTO - NOTNLM
OT  - EGFR
OT  - ICAM-1
OT  - amphiregulin
OT  - cell migration
OT  - osteosarcoma metastasis
COIS- CONFLICTS OF INTEREST The authors state no conflict of interest.
EDAT- 2015/10/28 06:00
MHDA- 2016/09/23 06:00
PMCR- 2015/12/01
CRDT- 2015/10/28 06:00
PHST- 2015/05/21 00:00 [received]
PHST- 2015/09/13 00:00 [accepted]
PHST- 2015/10/28 06:00 [entrez]
PHST- 2015/10/28 06:00 [pubmed]
PHST- 2016/09/23 06:00 [medline]
PHST- 2015/12/01 00:00 [pmc-release]
AID - 5679 [pii]
AID - 10.18632/oncotarget.5679 [doi]
PST - ppublish
SO  - Oncotarget. 2015 Dec 1;6(38):40880-95. doi: 10.18632/oncotarget.5679.