PMID- 26506436
OWN - NLM
STAT- MEDLINE
DCOM- 20160921
LR  - 20161126
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 7
IP  - 45
DP  - 2015 Nov 18
TI  - Enhanced In Vitro Biocompatibility of Chemically Modified Poly(dimethylsiloxane) 
      Surfaces for Stable Adhesion and Long-term Investigation of Brain Cerebral Cortex 
      Cells.
PG  - 25529-38
LID - 10.1021/acsami.5b09032 [doi]
AB  - Studies on the mammalian brain cerebral cortex have gained increasing importance 
      due to the relevance of the region in controlling critical higher brain 
      functions. Interactions between the cortical cells and surface extracellular 
      matrix (ECM) proteins play a pivotal role in promoting stable cell adhesion, 
      growth, and function. Poly(dimethylsiloxane) (PDMS) based platforms have been 
      increasingly used for on-chip in vitro cellular system analysis. However, the 
      inherent hydrophobicity of the PDMS surface has been unfavorable for any 
      long-term cell system investigations due to transitory physical adsorption of ECM 
      proteins on PDMS surfaces followed by eventual cell dislodgement due to poor 
      anchorage and viability. To address this critical issue, we employed the 
      (3-aminopropyl)triethoxysilane (APTES) based cross-linking strategy to stabilize 
      ECM protein immobilization on PDMS. The efficiency of surface modification in 
      supporting adhesion and long-term viability of neuronal and glial cells was 
      analyzed. The chemically modified surfaces showed a relatively higher cell 
      survival with an increased neurite length and neurite branching. These changes 
      were understood in terms of an increase in surface hydrophilicity, protein 
      stability, and cell-ECM protein interactions. The modification strategy could be 
      successfully applied for stable cortical cell culture on the PDMS microchip for 
      up to 3 weeks in vitro.
FAU - Kuddannaya, Shreyas
AU  - Kuddannaya S
AD  - School of Mechanical and Aerospace Engineering, Nanyang Technological University 
      , 50 Nanyang Avenue, N3.2-02-65, Singapore 639798, Singapore.
FAU - Bao, Jingnan
AU  - Bao J
AD  - School of Mechanical and Aerospace Engineering, Nanyang Technological University 
      , 50 Nanyang Avenue, N3.2-02-65, Singapore 639798, Singapore.
FAU - Zhang, Yilei
AU  - Zhang Y
AD  - School of Mechanical and Aerospace Engineering, Nanyang Technological University 
      , 50 Nanyang Avenue, N3.2-02-65, Singapore 639798, Singapore.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151105
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Biocompatible Materials)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Propylamines)
RN  - 0 (Proteins)
RN  - 0 (Silanes)
RN  - 059QF0KO0R (Water)
RN  - 63148-62-9 (baysilon)
RN  - L8S6UBW552 (amino-propyl-triethoxysilane)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/*pharmacology
MH  - Cell Adhesion/drug effects
MH  - Cells, Cultured
MH  - Cerebral Cortex/*cytology
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Materials Testing/*methods
MH  - Microfluidic Analytical Techniques
MH  - Neurons/cytology
MH  - Photoelectron Spectroscopy
MH  - Propylamines/chemistry
MH  - Proteins/chemistry
MH  - Rats, Sprague-Dawley
MH  - Silanes/chemistry
MH  - Surface Properties
MH  - Time Factors
MH  - Water/chemistry
OTO - NOTNLM
OT  - (3-aminopropyl)triethoxysilane (APTES)
OT  - cerebral cortex
OT  - glial cells
OT  - neurons
OT  - poly(dimethylsiloxane)
OT  - protein immobilization
EDAT- 2015/10/28 06:00
MHDA- 2016/09/23 06:00
CRDT- 2015/10/28 06:00
PHST- 2015/10/28 06:00 [entrez]
PHST- 2015/10/28 06:00 [pubmed]
PHST- 2016/09/23 06:00 [medline]
AID - 10.1021/acsami.5b09032 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2015 Nov 18;7(45):25529-38. doi: 
      10.1021/acsami.5b09032. Epub 2015 Nov 5.