PMID- 26506955
OWN - NLM
STAT- MEDLINE
DCOM- 20160519
LR  - 20231213
IS  - 0717-6287 (Electronic)
IS  - 0716-9760 (Print)
IS  - 0716-9760 (Linking)
VI  - 48
DP  - 2015 Oct 27
TI  - Functional disruption of human leukocyte antigen II in human embryonic stem cell.
PG  - 59
LID - 10.1186/s40659-015-0051-6 [doi]
LID - 59
AB  - BACKGROUND: Theoretically human embryonic stem cells (hESCs) have the capacity to 
      self-renew and differentiate into all human cell types. Therefore, the greatest 
      promise of hESCs-based therapy is to replace the damaged tissues of patients 
      suffering from traumatic or degenerative diseases by the exact same type of cells 
      derived from hESCs. Allograft immune rejection is one of the obstacles for 
      hESCs-based clinical applications. Human leukocyte antigen (HLA) II leads to 
      CD4(+) T cells-mediated allograft rejection. Hence, we focus on optimizing hESCs 
      for clinic application through gene modification. RESULTS: Transcription 
      activator-like effector nucleases (TALENs) were used to target MHC class II 
      transactivator (CIITA) in hESCs efficiently. CIITA (-/-) hESCs did not show any 
      difference in the differentiation potential and self-renewal capacity. Dendritic 
      cells (DCs) derived from CIITA (-/-) hESCs expressed CD83 and CD86 but without 
      the constitutive HLA II. Fibroblasts derived from CIITA (-/-) hESCs were 
      powerless in IFN-gamma inducible expression of HLA II. CONCLUSION: We generated HLA 
      II defected hESCs via deleting CIITA, a master regulator of constitutive and 
      IFN-gamma inducible expression of HLA II genes. CIITA (-/-) hESCs can differentiate 
      into tissue cells with non-HLA II expression. It's promising that CIITA (-/-) 
      hESCs-derived cells could be used in cell therapy (e.g., T cells and DCs) and 
      escape the attack of receptors' CD4(+) T cells, which are the main effector cells 
      of cellular immunity in allograft.
FAU - Chen, Haide
AU  - Chen H
AD  - College of Animal Science, Zhejiang University, Hangzhou, 310058, People's 
      Republic of China. Hyde@zju.edu.cn.
AD  - Zhejiang University School of Medicine, Hangzhou, 310058, People's Republic of 
      China. Hyde@zju.edu.cn.
FAU - Li, Yang
AU  - Li Y
AD  - College of Animal Science, Zhejiang University, Hangzhou, 310058, People's 
      Republic of China. Younglee@zju.edu.cn.
AD  - Zhejiang University School of Medicine, Hangzhou, 310058, People's Republic of 
      China. Younglee@zju.edu.cn.
FAU - Lin, Xijuan
AU  - Lin X
AD  - College of Animal Science, Zhejiang University, Hangzhou, 310058, People's 
      Republic of China. 11217009@zju.edu.cn.
AD  - Zhejiang University School of Medicine, Hangzhou, 310058, People's Republic of 
      China. 11217009@zju.edu.cn.
FAU - Cui, Di
AU  - Cui D
AD  - College of Animal Science, Zhejiang University, Hangzhou, 310058, People's 
      Republic of China. 21217007@zju.edu.cn.
AD  - Zhejiang University School of Medicine, Hangzhou, 310058, People's Republic of 
      China. 21217007@zju.edu.cn.
FAU - Cui, Chun
AU  - Cui C
AD  - Wuxi Medical School, Jiangnan University, Wuxi, 214122, People's Republic of 
      China. Cuichun@jiangnan.edu.cn.
FAU - Li, Hui
AU  - Li H
AD  - Xiangtan Center Hospital, Hunan, 411100, People's Republic of China. 
      Lihui_1973@126.com.
FAU - Xiao, Lei
AU  - Xiao L
AD  - College of Animal Science, Zhejiang University, Hangzhou, 310058, People's 
      Republic of China. Leixiao@zju.edu.cn.
AD  - Zhejiang University School of Medicine, Hangzhou, 310058, People's Republic of 
      China. Leixiao@zju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151027
PL  - England
TA  - Biol Res
JT  - Biological research
JID - 9308271
RN  - 0 (Antigens, CD)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Immunoglobulins)
RN  - 0 (MHC class II transactivator protein)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Trans-Activators)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.1.- (Deoxyribonucleases)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/metabolism
MH  - Antigens, CD/metabolism
MH  - B7-2 Antigen/metabolism
MH  - Cell Differentiation/*genetics
MH  - Cell Self Renewal
MH  - Dendritic Cells/metabolism
MH  - Deoxyribonucleases/classification/*metabolism
MH  - Embryoid Bodies/metabolism
MH  - Fibroblasts/metabolism
MH  - *Gene Deletion
MH  - Histocompatibility Antigens Class II/genetics
MH  - Human Embryonic Stem Cells/*metabolism
MH  - Humans
MH  - Immunoglobulins/metabolism
MH  - Immunohistochemistry
MH  - Interferon-gamma/metabolism
MH  - Karyotype
MH  - Membrane Glycoproteins/metabolism
MH  - Mice
MH  - Mice, SCID
MH  - Nuclear Proteins/*genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Teratoma
MH  - Trans-Activators/*genetics
MH  - Tumor Cells, Cultured
MH  - CD83 Antigen
PMC - PMC4624597
EDAT- 2015/10/29 06:00
MHDA- 2016/05/20 06:00
PMCR- 2015/10/27
CRDT- 2015/10/29 06:00
PHST- 2015/05/27 00:00 [received]
PHST- 2015/10/20 00:00 [accepted]
PHST- 2015/10/29 06:00 [entrez]
PHST- 2015/10/29 06:00 [pubmed]
PHST- 2016/05/20 06:00 [medline]
PHST- 2015/10/27 00:00 [pmc-release]
AID - 10.1186/s40659-015-0051-6 [pii]
AID - 51 [pii]
AID - 10.1186/s40659-015-0051-6 [doi]
PST - epublish
SO  - Biol Res. 2015 Oct 27;48:59. doi: 10.1186/s40659-015-0051-6.