PMID- 26508429
OWN - NLM
STAT- MEDLINE
DCOM- 20160914
LR  - 20181202
IS  - 1432-0800 (Electronic)
IS  - 0007-4861 (Linking)
VI  - 96
IP  - 1
DP  - 2016 Jan
TI  - A Study of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Induced Liver Injury in Jian Carp 
      (Cyprinus carpio var. Jian) Using Precision-Cut Liver Slices.
PG  - 55-61
LID - 10.1007/s00128-015-1683-5 [doi]
AB  - The aim of this study was to establish a model for the study of liver injury 
      induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in Jian carp using 
      precision-cut liver slices (PCLS). PCLS were treated with TCDD at concentrations 
      of 0, 0.05, 0.1, 0.3, and 0.6 mug/L for 6 h, followed by collection of the culture 
      supernatant and PCLS for analysis. Several biochemical indices were analyzed, 
      including glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase 
      (GOT), lactate dehydrogenase (LDH), superoxide dismutase (SOD), glutathione 
      peroxidase (GSH-Px), and malondialdehyde (MDA). Expression of mRNA was also 
      estimated for cytochrome P4501A (CYP1A), aryl hydrocarbon receptor2 (AhR2), and 
      aryl hydrocarbon receptor nuclear translocator2 (ARNT2). Results showed that some 
      significant effects (p < 0.05) in MDA, GSH-Px and PCLS viability were observed at 
      a TCDD concentration as low as 0.05 microg/L, and the observed effects increased with 
      exposure concentration. Following exposure to TCDD for 6 h at a concentration of 
      0.3 mug/L, significant increases (p < 0.01) in the content of GPT, GOT, MDA, and 
      LDH were observed, while SOD activity, GSH-Px activity, and PCLS viability were 
      decreased (p < 0.01 or p < 0.05). Exposure to 0.3 mug/L TCDD also resulted in 
      increased expression of mRNA for CYP1A, AhR2, and ARNT2. Overall, these results 
      provide evidence of TCDD-induced liver injury and oxidative stress in Jian carp. 
      These results also support the use of PCLS as an in vitro model for the 
      evaluation of hepatotoxicity in Jian carp.
FAU - Du, Jin-Liang
AU  - Du JL
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of 
      Fishery Sciences, Wuxi, 214081, China.
AD  - International Joint Research Laboratory for Fish Immunopharmacology, Freshwater 
      Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, 
      China.
FAU - Cao, Li-Ping
AU  - Cao LP
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of 
      Fishery Sciences, Wuxi, 214081, China.
AD  - International Joint Research Laboratory for Fish Immunopharmacology, Freshwater 
      Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, 
      China.
FAU - Liu, Ying-Juan
AU  - Liu YJ
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.
FAU - Jia, Rui
AU  - Jia R
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.
FAU - Yin, Guo-Jun
AU  - Yin GJ
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of 
      Fishery Sciences, Wuxi, 214081, China. yingj@ffrc.cn.
AD  - International Joint Research Laboratory for Fish Immunopharmacology, Freshwater 
      Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, 
      China. yingj@ffrc.cn.
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China. 
      yingj@ffrc.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151027
PL  - United States
TA  - Bull Environ Contam Toxicol
JT  - Bulletin of environmental contamination and toxicology
JID - 0046021
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Carps/*metabolism
MH  - Glutathione Peroxidase/metabolism
MH  - In Vitro Techniques/*methods
MH  - Liver/*drug effects/metabolism
MH  - Malondialdehyde/metabolism
MH  - Oxidation-Reduction
MH  - Oxidative Stress/*drug effects
MH  - Polychlorinated Dibenzodioxins/*toxicity
MH  - RNA, Messenger/metabolism
MH  - Receptors, Aryl Hydrocarbon/metabolism
MH  - Superoxide Dismutase/metabolism
OTO - NOTNLM
OT  - 2,3,7,8-Tetrachlorodibenzo-p-dioxin
OT  - ARNT2
OT  - AhR2
OT  - CYP1A
OT  - Jian Carp
EDAT- 2015/10/29 06:00
MHDA- 2016/09/15 06:00
CRDT- 2015/10/29 06:00
PHST- 2015/05/07 00:00 [received]
PHST- 2015/10/20 00:00 [accepted]
PHST- 2015/10/29 06:00 [entrez]
PHST- 2015/10/29 06:00 [pubmed]
PHST- 2016/09/15 06:00 [medline]
AID - 10.1007/s00128-015-1683-5 [pii]
AID - 10.1007/s00128-015-1683-5 [doi]
PST - ppublish
SO  - Bull Environ Contam Toxicol. 2016 Jan;96(1):55-61. doi: 
      10.1007/s00128-015-1683-5. Epub 2015 Oct 27.