PMID- 26509872 OWN - NLM STAT- MEDLINE DCOM- 20180117 LR - 20220408 IS - 1750-3639 (Electronic) IS - 1015-6305 (Print) IS - 1015-6305 (Linking) VI - 26 IP - 5 DP - 2016 Sep TI - New GABAergic Neurogenesis in the Hippocampal CA1 Region of a Gerbil Model of Long-Term Survival after Transient Cerebral Ischemic Injury. PG - 581-92 LID - 10.1111/bpa.12334 [doi] AB - We investigated the probability of newly generated neurons that could survive and mature in the ischemic hippocampal CA1 region (CA1) of a gerbil model of transient cerebral ischemia. Neuronal death was shown in the stratum pyramidale (SP) from 4 days post-ischemia, and a significant increase in NeuN-positive ((+) ) neurons was found in the SP at 180 days post-ischemia. 5-Bromo-2-deoxyuridine (BrdU)(+) cells were co-stained with NeuN and glutamic decarboxylase 67 (GAD67). Brain-derived neurotrophic factor (BDNF) immunoreactivity and protein level was shown in nonpyramidal cells from 4 days post-ischemia, and the immunoreactivity was strong at 30 days post-ischemia and not significantly changed until 180 days post-ischemia. Furthermore, TrkB immunoreactivity was co-stained with GAD67 when we examined at 180 days post-ischemia. Myelin basic protein (MBP)(+) nerve fibers were reduced at 4 days post-ischemia and maintained until 60 days post-ischemia, and MBP immunoreactivity and levels were significantly increased at 180 days post-ischemia. In the passive avoidance test, cognitive dysfunction was improved at 180 days post-ischemia. These results suggest that the differentiation of neural progenitor cells into new GABAergic neurons may be promoted via BDNF in the ischemic CA1 and that the neurogenesis may partially mediate the recovery of cognitive impairments via increasing myelinated nerve fibers. CI - (c) 2015 International Society of Neuropathology. FAU - Lee, Jae-Chul AU - Lee JC AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea. FAU - Park, Joon Ha AU - Park JH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea. FAU - Ahn, Ji Hyeon AU - Ahn JH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea. FAU - Kim, In Hye AU - Kim IH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea. FAU - Cho, Jeong Hwi AU - Cho JH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea. FAU - Choi, Jung Hoon AU - Choi JH AD - Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon, 200-701, South Korea. FAU - Yoo, Ki-Yeon AU - Yoo KY AD - Department of Oral Anatomy, College of Dentistry, Gangneung-Wonju National University, Gangneung, 210-702, South Korea. FAU - Lee, Choong Hyun AU - Lee CH AD - Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, 330-714, South Korea. FAU - Hwang, In Koo AU - Hwang IK AD - Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea. FAU - Cho, Jun Hwi AU - Cho JH AD - Department of Emergency Medicine, and Institute of Medical Sciences, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea. FAU - Kwon, Young-Guen AU - Kwon YG AD - Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, 120-749, South Korea. FAU - Kim, Young-Myeong AU - Kim YM AD - Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea. FAU - Kang, Il Jun AU - Kang IJ AD - Department of Food Science and Nutrition, Hallym University, Chuncheon, 200-702, South Korea. FAU - Won, Moo-Ho AU - Won MH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea. LA - eng PT - Journal Article DEP - 20151214 PL - Switzerland TA - Brain Pathol JT - Brain pathology (Zurich, Switzerland) JID - 9216781 RN - 0 (AIF1 protein, human) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Calcium-Binding Proteins) RN - 0 (DNA-Binding Proteins) RN - 0 (Glial Fibrillary Acidic Protein) RN - 0 (Microfilament Proteins) RN - 0 (Myelin Basic Protein) RN - EC 4.1.1.15 (Glutamate Decarboxylase) RN - EC 4.1.1.15 (glutamate decarboxylase 1) RN - EC 4.2.1.11 (Phosphopyruvate Hydratase) RN - G34N38R2N1 (Bromodeoxyuridine) SB - IM MH - Animals MH - Avoidance Learning MH - Brain Ischemia/*mortality/*pathology MH - Brain-Derived Neurotrophic Factor/metabolism MH - Bromodeoxyuridine/metabolism MH - CA1 Region, Hippocampal/*pathology MH - Calcium-Binding Proteins MH - Cell Count MH - DNA-Binding Proteins/metabolism MH - Disease Models, Animal MH - GABAergic Neurons/*physiology MH - Gene Expression Regulation/physiology MH - Gerbillinae MH - Glial Fibrillary Acidic Protein/metabolism MH - Glutamate Decarboxylase/metabolism MH - Male MH - Microfilament Proteins MH - Myelin Basic Protein/metabolism MH - Neurogenesis/*physiology MH - Phosphopyruvate Hydratase/metabolism MH - Reaction Time MH - Time Factors PMC - PMC8029099 OTO - NOTNLM OT - GABAergic neuron OT - hippocampal CA1 OT - myelinated nerve fibers OT - neurogenesis OT - transient cerebral ischemia EDAT- 2015/10/29 06:00 MHDA- 2018/01/18 06:00 PMCR- 2015/12/14 CRDT- 2015/10/29 06:00 PHST- 2015/06/01 00:00 [received] PHST- 2015/10/19 00:00 [accepted] PHST- 2015/10/29 06:00 [entrez] PHST- 2015/10/29 06:00 [pubmed] PHST- 2018/01/18 06:00 [medline] PHST- 2015/12/14 00:00 [pmc-release] AID - BPA12334 [pii] AID - 10.1111/bpa.12334 [doi] PST - ppublish SO - Brain Pathol. 2016 Sep;26(5):581-92. doi: 10.1111/bpa.12334. Epub 2015 Dec 14.