PMID- 26510865 OWN - NLM STAT- MEDLINE DCOM- 20161005 LR - 20181113 IS - 1529-7268 (Electronic) IS - 0006-3363 (Print) IS - 0006-3363 (Linking) VI - 93 IP - 6 DP - 2015 Dec TI - Effects of High-Butterfat Diet on Embryo Implantation in Female Rats Exposed to Bisphenol A. PG - 147 LID - 10.1095/biolreprod.115.131433 [doi] LID - 147 AB - Bisphenol A (BPA) is an endocrine disruptor associated with poor pregnancy outcomes in human and rodents. The effects of butterfat diets on embryo implantation and whether it modifies BPA's actions are currently unknown. We aimed to determine the effects of butterfat diet on embryo implantation success in female rats exposed to an environmentally relevant dose of BPA. Female Sprague-Dawley rats were exposed to dietary butterfat (10% or 39% kcal/kg body weight [BW]) in the presence or absence of BPA (250 mug/kg BW) or ethinylestradiol (0.1 mug/kg BW) shortly before and during pregnancy to assess embryo implantation potentials by preimplantation development and transport, in vitro blastulation, outgrowth, and implantation. On gestational day (GD) 4.5, rats treated with BPA alone had higher serum total BPA level (2.3-3.7 ng/ml). They had more late-stage preimplantation embryos, whereas those receiving high butterfat (HBF) diet had the most advanced-stage embryos; dams cotreated with HBF and BPA had the most number of advanced embryos. BPA markedly delayed embryo transport to the uterus, but neither amount of butterfat had modifying effects. An in vitro implantation assay showed HBF doubled the outgrowth area, with BPA having no effect. In vivo, BPA reduced the number of implanted embryos on GD8, and cotreatment with HBF eliminated this adverse effect. HBF diet overall resulted in more and larger GD8 embryos. This study reveals the implantation disruptive effects of maternal exposure to an environmentally relevant dose of BPA and identifies HBF diet as a modifier of BPA in promoting early embryonic health. CI - (c) 2015 by the Society for the Study of Reproduction, Inc. FAU - Martinez, Alan M AU - Martinez AM AD - Division of Reproductive Endocrinology & Infertility, Department of Obstetrics & Gynecology, University of Cincinnati College of Medicine, Cincinnati, Ohio. FAU - Cheong, Ana AU - Cheong A AD - Division of Environmental Genetics and Molecular Toxicology, Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, Ohio Center for Environmental Genetics, University of Cincinnati College of Medicine, Cincinnati, Ohio. FAU - Ying, Jun AU - Ying J AD - Division of Biostatistics and Bioinformatics, Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, Ohio. FAU - Xue, Jingchuan AU - Xue J AD - Wadsworth Center, New York State Department of Health, and Department of Environmental Health Sciences, School of Public Health, State University of New York at Albany, Albany, New York. FAU - Kannan, Kurunthachalam AU - Kannan K AD - Wadsworth Center, New York State Department of Health, and Department of Environmental Health Sciences, School of Public Health, State University of New York at Albany, Albany, New York Biochemistry Department, Faculty of Science and Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia. FAU - Leung, Yuet-Kin AU - Leung YK AD - Division of Environmental Genetics and Molecular Toxicology, Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, Ohio Center for Environmental Genetics, University of Cincinnati College of Medicine, Cincinnati, Ohio Cincinnati Cancer Center, Cincinnati, Ohio. FAU - Thomas, Michael A AU - Thomas MA AD - Division of Reproductive Endocrinology & Infertility, Department of Obstetrics & Gynecology, University of Cincinnati College of Medicine, Cincinnati, Ohio. FAU - Ho, Shuk-Mei AU - Ho SM AD - Division of Environmental Genetics and Molecular Toxicology, Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, Ohio Center for Environmental Genetics, University of Cincinnati College of Medicine, Cincinnati, Ohio Cincinnati Cancer Center, Cincinnati, Ohio Cincinnati Veteran Affairs Hospital Medical Center, Cincinnati, Ohio shuk-mei.ho@uc.edu. LA - eng GR - U01 ES020988/ES/NIEHS NIH HHS/United States GR - U01ES019480/ES/NIEHS NIH HHS/United States GR - P30 ES006096/ES/NIEHS NIH HHS/United States GR - U01ES020988/ES/NIEHS NIH HHS/United States GR - P30ES006096/ES/NIEHS NIH HHS/United States GR - I01 BX000675/BX/BLRD VA/United States GR - U01 ES019480/ES/NIEHS NIH HHS/United States PT - Journal Article DEP - 20151028 PL - United States TA - Biol Reprod JT - Biology of reproduction JID - 0207224 RN - 0 (Benzhydryl Compounds) RN - 0 (Ghee) RN - 0 (Phenols) RN - 423D2T571U (Ethinyl Estradiol) RN - MLT3645I99 (bisphenol A) SB - IM MH - Animals MH - Benzhydryl Compounds/*pharmacology MH - *Diet MH - Embryo Implantation/*drug effects MH - Embryonic Development/*drug effects MH - Ethinyl Estradiol/pharmacology MH - Female MH - *Ghee MH - Phenols/*pharmacology MH - Pregnancy MH - Rats MH - Rats, Sprague-Dawley PMC - PMC4712698 OTO - NOTNLM OT - bisphenol A OT - blastocyst outgrowth OT - embryo implantation OT - high butterfat diet OT - preimplantation embryo development EDAT- 2015/10/30 06:00 MHDA- 2016/10/07 06:00 PMCR- 2016/12/01 CRDT- 2015/10/30 06:00 PHST- 2015/05/12 00:00 [received] PHST- 2015/10/22 00:00 [accepted] PHST- 2015/10/30 06:00 [entrez] PHST- 2015/10/30 06:00 [pubmed] PHST- 2016/10/07 06:00 [medline] PHST- 2016/12/01 00:00 [pmc-release] AID - biolreprod.115.131433 [pii] AID - Manuscript Number: BIOLREPROD/2015/131433 [pii] AID - 10.1095/biolreprod.115.131433 [doi] PST - ppublish SO - Biol Reprod. 2015 Dec;93(6):147. doi: 10.1095/biolreprod.115.131433. Epub 2015 Oct 28.