PMID- 26511903
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1600-079X (Electronic)
IS  - 0742-3098 (Linking)
VI  - 60
IP  - 1
DP  - 2016 Jan
TI  - The neuroprotective and anti-apoptotic effects of melatonin on hemolytic 
      hyperbilirubinemia-induced oxidative brain damage.
PG  - 74-83
LID - 10.1111/jpi.12292 [doi]
AB  - Melatonin exerts protection in several inflammatory and neurodegenerative 
      disorders. To investigate the neuroprotective effects of melatonin in an 
      experimental hemolysis-induced hyperbilirubinemia, newborn Sprague-Dawley rats 
      (25-40 g, n = 72) were injected with phenylhydrazine hydrochloride (PHZ; 75 
      mg/kg) and the injections were repeated at the 24th hour. Rats were treated with 
      saline or melatonin (10 mg/kg) 30 min before the first and second PHZ injections 
      and 24 h after the 2nd PHZ injections. Control rats (n = 24) were injected with 
      saline, but not PHZ. At sixth hours after the last injections of saline or 
      melatonin, all rats were decapitated. Tumor necrosis factor (TNF)-alpha, IL-1beta, IL-10 
      and brain-derived neurotrophic factor (BDNF) and S100B levels in the plasma were 
      measured. Brain tissue malondialdehyde (MDA), glutathione (GSH) levels and 
      myeloperoxidase (MPO) activities were measured, and brain tissues were evaluated 
      for apoptosis by TUNEL method. In the saline-treated PHZ group, hemoglobin, 
      hematocrit levels were reduced, and total/direct bilirubin levels were elevated 
      when compared to control group. Increased plasma TNF-alpha, IL-1beta levels, along with 
      decreased BDNF, S100B and IL-10 values were observed in the saline-treated PHZ 
      group, while these changes were all reversed in the melatonin-treated group. 
      Increased MDA levels and MPO activities in the brain tissues of saline-treated 
      hyperbilirubinemic rats, concomitant with depleted brain GSH stores, were also 
      reversed in the melatonin-treated hyperbilirubinemic rats. Increased TUNEL(+) 
      cells in the hippocampus of saline-treated PHZ group were reduced by melatonin 
      treatment. Melatonin exerts neuroprotective and anti-apoptotic effects on the 
      oxidative neuronal damage of the newborn rats with hemolysis and 
      hyperbilirubinemia.
CI  - (c) 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Pazar, Asilay
AU  - Pazar A
AD  - Department of Paediatrics, Marmara University School of Medicine, Istanbul, 
      Turkey.
FAU - Kolgazi, Meltem
AU  - Kolgazi M
AD  - Department of Physiology, Acibadem University School of Medicine, Istanbul, 
      Turkey.
FAU - Memisoglu, Asli
AU  - Memisoglu A
AD  - Division of Neonatology, Department of Paediatrics, Marmara University School of 
      Medicine, Istanbul, Turkey.
FAU - Bahadir, Elif
AU  - Bahadir E
AD  - Department of Physiology, Marmara University School of Medicine, Istanbul, 
      Turkey.
FAU - Sirvanci, Serap
AU  - Sirvanci S
AD  - Department of Histology and Embryology, Marmara University School of Medicine, 
      Istanbul, Turkey.
FAU - Yaman, Akan
AU  - Yaman A
AD  - Division of Neonatology, Department of Paediatrics, Marmara University School of 
      Medicine, Istanbul, Turkey.
FAU - Yegen, Berrak C
AU  - Yegen BC
AD  - Department of Physiology, Marmara University School of Medicine, Istanbul, 
      Turkey.
FAU - Ozek, Eren
AU  - Ozek E
AD  - Division of Neonatology, Department of Paediatrics, Marmara University School of 
      Medicine, Istanbul, Turkey.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151130
PL  - England
TA  - J Pineal Res
JT  - Journal of pineal research
JID - 8504412
RN  - 0 (Cytokines)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuroprotective Agents)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Brain Injuries/metabolism/pathology/*prevention & control
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Jaundice/*drug therapy/metabolism/pathology
MH  - Melatonin/*pharmacology
MH  - Nerve Tissue Proteins/metabolism
MH  - Neuroprotective Agents/*pharmacology
MH  - Oxidation-Reduction/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - apoptosis
OT  - hemolysis
OT  - hyperbilirubinemia
OT  - melatonin
OT  - newborn
EDAT- 2015/10/30 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/10/30 06:00
PHST- 2015/09/04 00:00 [received]
PHST- 2015/10/26 00:00 [accepted]
PHST- 2015/10/30 06:00 [entrez]
PHST- 2015/10/30 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1111/jpi.12292 [doi]
PST - ppublish
SO  - J Pineal Res. 2016 Jan;60(1):74-83. doi: 10.1111/jpi.12292. Epub 2015 Nov 30.