PMID- 26512101
OWN - NLM
STAT- MEDLINE
DCOM- 20160229
LR  - 20220318
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 112
IP  - 45
DP  - 2015 Nov 10
TI  - KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis 
      cofactor HYL1.
PG  - 14096-101
LID - 10.1073/pnas.1512865112 [doi]
AB  - The biogenesis of microRNAs (miRNAs), which regulate mRNA abundance through 
      posttranscriptional silencing, comprises multiple well-orchestrated processing 
      steps. We have identified the Arabidopsis thaliana K homology (KH) domain protein 
      REGULATOR OF CBF GENE EXPRESSION 3 (RCF3) as a cofactor affecting miRNA 
      biogenesis in specific plant tissues. MiRNA and miRNA-target levels were reduced 
      in apex-enriched samples of rcf3 mutants, but not in other tissues. 
      Mechanistically, RCF3 affects miRNA biogenesis through nuclear interactions with 
      the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2). These 
      interactions are essential to regulate the phosphorylation status, and thus the 
      activity, of the double-stranded RNA binding protein and DICER-LIKE1 (DCL1) 
      cofactor HYPONASTIC LEAVES1 (HYL1).
FAU - Karlsson, Patricia
AU  - Karlsson P
AD  - Max Planck Institute for Developmental Biology, D-72076 Tubingen, Germany;
FAU - Christie, Michael Danger
AU  - Christie MD
AD  - Max Planck Institute for Developmental Biology, D-72076 Tubingen, Germany;
FAU - Seymour, Danelle K
AU  - Seymour DK
AD  - Max Planck Institute for Developmental Biology, D-72076 Tubingen, Germany;
FAU - Wang, Huan
AU  - Wang H
AD  - Max Planck Institute for Developmental Biology, D-72076 Tubingen, Germany;
FAU - Wang, Xi
AU  - Wang X
AD  - Max Planck Institute for Developmental Biology, D-72076 Tubingen, Germany;
FAU - Hagmann, Jorg
AU  - Hagmann J
AD  - Max Planck Institute for Developmental Biology, D-72076 Tubingen, Germany;
FAU - Kulcheski, Franceli
AU  - Kulcheski F
AD  - Max Planck Institute for Developmental Biology, D-72076 Tubingen, Germany;
FAU - Manavella, Pablo Andres
AU  - Manavella PA
AUID- ORCID: 0000-0003-3589-4414
AD  - Max Planck Institute for Developmental Biology, D-72076 Tubingen, Germany; 
      Instituto de Agrobiotecnologia del Litoral, Universidad Nacional del Litoral - 
      Consejo Nacional de Investigaciones Cientificas y Tecnicas, 3000 Santa Fe, 
      Argentina pablomanavella@ial.santafe-conicet.gov.ar.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151028
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (HYL1 protein, Arabidopsis)
RN  - 0 (MicroRNAs)
RN  - 0 (RCF3 protein, Arabidopsis)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Arabidopsis/*metabolism
MH  - Arabidopsis Proteins/*metabolism
MH  - Base Sequence
MH  - DNA Primers/genetics
MH  - Luciferases
MH  - MicroRNAs/*biosynthesis
MH  - Molecular Sequence Data
MH  - RNA-Binding Proteins/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Transgenes/genetics
PMC - PMC4653147
OTO - NOTNLM
OT  - Arabidopsis thaliana
OT  - HYL1
OT  - gene silencing
OT  - micro RNA biogenesis
OT  - phosphorylation
COIS- The authors declare no conflict of interest.
EDAT- 2015/10/30 06:00
MHDA- 2016/03/02 06:00
PMCR- 2016/05/10
CRDT- 2015/10/30 06:00
PHST- 2015/10/30 06:00 [entrez]
PHST- 2015/10/30 06:00 [pubmed]
PHST- 2016/03/02 06:00 [medline]
PHST- 2016/05/10 00:00 [pmc-release]
AID - 1512865112 [pii]
AID - 201512865 [pii]
AID - 10.1073/pnas.1512865112 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2015 Nov 10;112(45):14096-101. doi: 
      10.1073/pnas.1512865112. Epub 2015 Oct 28.