PMID- 26513157
OWN - NLM
STAT- MEDLINE
DCOM- 20160930
LR  - 20151219
IS  - 1879-0054 (Electronic)
IS  - 0739-7240 (Linking)
VI  - 54
DP  - 2016 Jan
TI  - MicroRNA-10b suppresses goat granulosa cell proliferation by targeting 
      brain-derived neurotropic factor.
PG  - 60-7
LID - S0739-7240(15)00103-4 [pii]
LID - 10.1016/j.domaniend.2015.09.005 [doi]
AB  - Brain-derived neurotropic factor (BDNF) and its high-affinity receptor, tyrosine 
      kinase receptor B, have been assumed to be involved in female reproduction and 
      have recently shown to play an essential role in follicle activation and oocyte 
      maturation. In this study, we analyzed the expression of miR-10b and BDNF in the 
      ovary and discovered that the expression of miR-10b was higher in monotocous goat 
      ovaries than in polytocous goat ovaries, whereas the expression pattern of BDNF 
      in ovary was opposite. Moreover, human chorionic gonadotropin induced rapid and 
      transient expression of BDNF messenger RNA and protein. In contrast, human 
      chorionic gonadotropin upregulated miR-10b expression in a time-dependent manner. 
      The BDNF gene was identified as a direct target of miR-10b using a 
      dual-luciferase reporter assay. Transfection of granulosa cells with miR-10b 
      decreased BDNF messenger RNA and protein levels. MiR-10b overexpression inhibited 
      cell proliferation, whereas BDNF promoted cell proliferation. However, a combined 
      treatment with miR-10b and BDNF promoted cell proliferation, indicating that the 
      reintroduction of BDNF reversed the suppressive effect of miR-10b. These results 
      demonstrate that miR-10b downregulates BDNF expression in granulosa cells by 
      directly targeting the 3' untranslated regions and plays an important role in 
      inhibiting granulosa cell proliferation by targeting BDNF.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Peng, J Y
AU  - Peng JY
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, P.R. China.
FAU - An, X P
AU  - An XP
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, P.R. China.
FAU - Fang, F
AU  - Fang F
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, P.R. China.
FAU - Gao, K X
AU  - Gao KX
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, P.R. China.
FAU - Xin, H Y
AU  - Xin HY
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, P.R. China.
FAU - Han, P
AU  - Han P
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, P.R. China.
FAU - Bao, L J
AU  - Bao LJ
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, P.R. China.
FAU - Ma, H D
AU  - Ma HD
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, P.R. China.
FAU - Cao, B Y
AU  - Cao BY
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, P.R. China. Electronic address: caobinyun@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151023
PL  - United States
TA  - Domest Anim Endocrinol
JT  - Domestic animal endocrinology
JID - 8505191
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/analysis/*genetics/physiology
MH  - Cell Proliferation/*drug effects/physiology
MH  - Cells, Cultured
MH  - Chorionic Gonadotropin/pharmacology
MH  - Down-Regulation
MH  - Female
MH  - Gene Expression/drug effects
MH  - *Goats
MH  - Granulosa Cells/*cytology/metabolism
MH  - MicroRNAs/genetics/*pharmacology
MH  - Ovary/chemistry/metabolism
MH  - RNA, Messenger/analysis
OTO - NOTNLM
OT  - Brain-derived neurotropic factor
OT  - Granulosa cell
OT  - Human chorionic gonadotropin
OT  - miR-10b
EDAT- 2015/10/30 06:00
MHDA- 2016/10/01 06:00
CRDT- 2015/10/30 06:00
PHST- 2015/07/24 00:00 [received]
PHST- 2015/09/21 00:00 [revised]
PHST- 2015/09/21 00:00 [accepted]
PHST- 2015/10/30 06:00 [entrez]
PHST- 2015/10/30 06:00 [pubmed]
PHST- 2016/10/01 06:00 [medline]
AID - S0739-7240(15)00103-4 [pii]
AID - 10.1016/j.domaniend.2015.09.005 [doi]
PST - ppublish
SO  - Domest Anim Endocrinol. 2016 Jan;54:60-7. doi: 10.1016/j.domaniend.2015.09.005. 
      Epub 2015 Oct 23.