PMID- 26513879
OWN - NLM
STAT- MEDLINE
DCOM- 20151215
LR  - 20200929
IS  - 0392-2936 (Print)
IS  - 0392-2936 (Linking)
VI  - 36
IP  - 5
DP  - 2015
TI  - Extracellular matrix metalloproteinase inducer (EMMPRIN) remodels the 
      extracellular matrix through enhancing matrix metalloproteinases (MMPs) and 
      inhibiting tissue inhibitors of MMPs expression in HPV-positive cervical cancer 
      cells.
PG  - 539-45
AB  - PUPOSE OF INVESTIGATION: To study the expression of extracellular matrix 
      metalloproteinase inducer (EMMPRIN), matrix metalloproteinases (MMPs), and tissue 
      inhibitors of MMP (TIMPs) in uterine cervical cancer cell lines in vitro. 
      MATERIALS AND METHODS: EMMPRIN, MMPs, and TIMPs expression were assessed by 
      Western blot and real-time RT-PCR from cervical carcinoma SiHa, HeLa, and C33-A 
      cells. RESULTS: EMMPRIN recombinant significantly increased MMP-2, MMP-9 protein 
      and mRNA expression in SiHa and Hela cells, but not in C33-A cells by Western 
      blot analysis and real-time RT-PCR. EMMPRIN recombinant significantly inhibited 
      TIMP-1 protein and mRNA levels in SiHa and Hela cells, but not in C33-A cells. 
      There was no difference on the TIMP-2 expression in those cells with the 
      treatment of EMMPRIN recombinant. EMMPRIN RNAi decreased MMP-2 and MMP-9 and 
      increased TIMP-1 expression in SiHa and HeLa cells, but not in C33-A cells. There 
      was no change on the expression of TIMP-2 mRNA levels in SiHa, HeLa and C33-A 
      cells transfected with siEMMPRIN. CONCLUSION: EMMPRIN may induce MMP-2 and MMP-9, 
      and downregulate TIMP-1 in HPV-positive cervical cancer cells in vitro.
FAU - Xu, Q
AU  - Xu Q
FAU - Cao, X
AU  - Cao X
FAU - Pan, J
AU  - Pan J
FAU - Ye, Y
AU  - Ye Y
FAU - Xie, Y
AU  - Xie Y
FAU - Ohara, N
AU  - Ohara N
FAU - Ji, H
AU  - Ji H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - Eur J Gynaecol Oncol
JT  - European journal of gynaecological oncology
JID - 8100357
RN  - 0 (BSG protein, human)
RN  - 0 (TIMP1 protein, human)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 136894-56-9 (Basigin)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Basigin/*physiology
MH  - Cell Line, Tumor
MH  - Extracellular Matrix/*metabolism
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinases/*biosynthesis/genetics
MH  - Papillomaviridae/*isolation & purification
MH  - Tissue Inhibitor of Metalloproteinase-1/analysis/*antagonists & 
      inhibitors/genetics
MH  - Uterine Cervical Neoplasms/*metabolism/pathology/virology
EDAT- 2015/10/31 06:00
MHDA- 2015/12/17 06:00
CRDT- 2015/10/31 06:00
PHST- 2015/10/31 06:00 [entrez]
PHST- 2015/10/31 06:00 [pubmed]
PHST- 2015/12/17 06:00 [medline]
PST - ppublish
SO  - Eur J Gynaecol Oncol. 2015;36(5):539-45.