PMID- 26513923 OWN - NLM STAT- MEDLINE DCOM- 20151229 LR - 20181202 IS - 0125-1562 (Print) IS - 0125-1562 (Linking) VI - 46 IP - 2 DP - 2015 Mar TI - PHYLOGENETIC ANALYSIS REVEALS GENETIC VARIATIONS OF DENSOVIRUS ISOLATED FROM FIELD MOSQUITOES IN BANGKOK AND SURROUNDING REGIONS. PG - 207-14 AB - Screening for densoviruses (DNVs) from Aedes, Culex and Toxorhynchites mosquitoes collected in Bangkok and surrounding regions identified two clades of Aedes DNV; Ae. aegypti DNV (AaeDNV) and Ae. albopictus DNV (AalDNV) by PCR-restriction fragment length polymorphism (PCR-RFLP). From nucleotide sequencing and phylogenetic analysis of PCR amplicons of a fragment of DNV capsid gene, these DNVs were shown to be new DNV genetic variations similar to AaeDNV. Isolation and identification of densoviruses from indigenous field mosquitoes reside in natural habitat should be helpful in monitoring the distribution of DNVs in important mosquitoes, especially Ae. aegypti and Ae. albopictus, vector for dengue and yellow fever viruses. FAU - Boonnak, Kobporn AU - Boonnak K FAU - Suttitheptumrong, Aroonroong AU - Suttitheptumrong A FAU - Jotekratok, Ubonwan AU - Jotekratok U FAU - Pattanakitsakul, Sa-Nga AU - Pattanakitsakul SN LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Thailand TA - Southeast Asian J Trop Med Public Health JT - The Southeast Asian journal of tropical medicine and public health JID - 0266303 RN - 0 (DNA, Viral) SB - IM MH - Aedes/*virology MH - Animals MH - Base Sequence MH - Culex/*virology MH - Culicidae/virology MH - DNA, Viral/*analysis MH - Densovirus/*genetics MH - *Genetic Variation MH - Phylogeny MH - Polymerase Chain Reaction MH - Polymorphism, Restriction Fragment Length MH - Thailand EDAT- 2015/10/31 06:00 MHDA- 2015/12/30 06:00 CRDT- 2015/10/31 06:00 PHST- 2015/10/31 06:00 [entrez] PHST- 2015/10/31 06:00 [pubmed] PHST- 2015/12/30 06:00 [medline] PST - ppublish SO - Southeast Asian J Trop Med Public Health. 2015 Mar;46(2):207-14.