PMID- 26514535
OWN - NLM
STAT- MEDLINE
DCOM- 20160913
LR  - 20220408
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Linking)
VI  - 16
IP  - 16
DP  - 2015
TI  - Importance of FISH combined with Morphology, Immunophenotype and Cytogenetic 
      Analysis of Childhood/ Adult Acute Lymphoblastic Leukemia in Omani Patients.
PG  - 7343-50
AB  - Genetic changes associated with acute lymphoblastic leukemia (ALL) provide very 
      important diagnostic and prognostic information with a direct impact on patient 
      management. Detection of chromosome abnormalities by conventional cytogenetics 
      combined with fluorescence in situ hybridization (FISH) play a very significant 
      role in assessing risk stratification. Identification of specific chromosome 
      abnormalities has led to the recognition of genetic subgroups based on reciprocal 
      translocations, deletions and modal number in B or T-cell ALL. In the last twelve 
      years 102 newly diagnosed childhood/adult ALL bone marrow samples were analysed 
      for chromosomal abnormalities with conventional G-banding, and FISH (selected 
      cases) using specific probes in our hospital. G-banded karyotype analysis found 
      clonal numerical and/or structural chromosomal aberrations in 74.2% of cases. 
      Patients with pseudodiploidy represented the most frequent group (38.7%) followed 
      by high hyperdiploidy group (12.9%), low hyperdiploidy group (9.7%), hypodiploidy 
      (<46) group (9.7%) and high hypertriploidy group (3.2%). The highest observed 
      numerical chromosomal alteration was high hyperdiploidy (12.9%) with abnormal 
      karyotypes while abnormal 12p (7.5%) was the highest observed structural 
      abnormality followed by t(12;21)(p13.3;q22) resulting in ETV6/RUNX1 fusion (5.4%) 
      and t(9;22)(q34.1;q11.2) resulting in BCR/ABL1 fusion (4.3%). Interestingly, we 
      identified 16 cases with rare and complex structural aberrations. Application of 
      the FISH technique produced major improvements in the sensitivity and accuracy of 
      cytogenetic analysis with ALL patients. In conclusion it confirmed heterogeneity 
      of ALL by identifying various recurrent chromosomal aberrations along with 
      non-specific rearrangements and their association with specific immunophenotypes. 
      This study pool is representative of paediatric/adult ALL patients in Oman.
FAU - Goud, Tadakal Mallana
AU  - Goud TM
AD  - National Genetic Centre, Muscat, Sultanate of Oman E-mail : tmgoud99@gmail.com.
FAU - Al Salmani, Kamla Khalfan
AU  - Al Salmani KK
FAU - Al Harasi, Salma Mohammed
AU  - Al Harasi SM
FAU - Al Musalhi, Muhanna
AU  - Al Musalhi M
FAU - Wasifuddin, Shah Mohammed
AU  - Wasifuddin SM
FAU - Rajab, Anna
AU  - Rajab A
LA  - eng
PT  - Journal Article
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Cytogenetic Analysis/*methods
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Immunophenotyping/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Oman/epidemiology
MH  - Precursor Cell Lymphoblastic 
      Leukemia-Lymphoma/epidemiology/*genetics/immunology/pathology
MH  - Prognosis
MH  - Translocation, Genetic/genetics
MH  - Young Adult
EDAT- 2015/10/31 06:00
MHDA- 2016/09/14 06:00
CRDT- 2015/10/31 06:00
PHST- 2015/10/31 06:00 [entrez]
PHST- 2015/10/31 06:00 [pubmed]
PHST- 2016/09/14 06:00 [medline]
AID - 10.7314/apjcp.2015.16.16.7343 [doi]
PST - ppublish
SO  - Asian Pac J Cancer Prev. 2015;16(16):7343-50. doi: 10.7314/apjcp.2015.16.16.7343.