PMID- 26517399
OWN - NLM
STAT- MEDLINE
DCOM- 20160425
LR  - 20191210
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 487
DP  - 2016 Jan
TI  - The human endogenous retrovirus K(HML-2) has a broad envelope-mediated cellular 
      tropism and is prone to inhibition at a post-entry, pre-integration step.
PG  - 121-8
LID - S0042-6822(15)00442-0 [pii]
LID - 10.1016/j.virol.2015.10.014 [doi]
AB  - The HERV-K(HML-2) family is the most recent addition to the collection of human 
      endogenous retroviruses. It comprises proviruses that encode functional proteins 
      that can assemble into replication defective particles carrying the envelope 
      protein. Using a reconstituted HERV-K113 envelope sequence, we have analyzed its 
      ability to mediate entry into a set of 33 cell lines from 10 species. Of these, 
      30 were permissive, demonstrating an amphotropism consistent with a broad 
      expression of receptor protein(s). In an initial effort to identify a receptor 
      for HERV-K(HML-2) we investigated whether transferrin receptor 1 and 
      hyaluronidase 2, known cellular receptors of the closely related betaretroviruses 
      mouse mammary tumor virus (MMTV) and Jaagsiekte sheep retrovirus (JSRV), could 
      facilitate HERV-K(HML-2) entry. However, neither of these proteins could serve as 
      a receptor for HERV-K(HML-2). Moreover, during attempts to further characterize 
      the tropism of HERV-K(HML-2), we identified a cellular activity that inhibits 
      infection at a post-entry, pre-integration step.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Kramer, Philipp
AU  - Kramer P
AD  - Robert Koch Institute, Division for HIV and Other Retroviruses, Nordufer 20, 
      13353 Berlin, Germany.
FAU - Lausch, Veronika
AU  - Lausch V
AD  - Robert Koch Institute, Division for HIV and Other Retroviruses, Nordufer 20, 
      13353 Berlin, Germany.
FAU - Volkwein, Alexander
AU  - Volkwein A
AD  - Robert Koch Institute, Division for HIV and Other Retroviruses, Nordufer 20, 
      13353 Berlin, Germany.
FAU - Hanke, Kirsten
AU  - Hanke K
AD  - Robert Koch Institute, Division for HIV and Other Retroviruses, Nordufer 20, 
      13353 Berlin, Germany.
FAU - Hohn, Oliver
AU  - Hohn O
AD  - Robert Koch Institute, Division for HIV and Other Retroviruses, Nordufer 20, 
      13353 Berlin, Germany.
FAU - Bannert, Norbert
AU  - Bannert N
AD  - Robert Koch Institute, Division for HIV and Other Retroviruses, Nordufer 20, 
      13353 Berlin, Germany. Electronic address: BannertN@rki.de.
LA  - eng
PT  - Journal Article
DEP - 20151027
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antigens, CD)
RN  - 0 (CD71 antigen)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Receptors, Transferrin)
RN  - 0 (Receptors, Virus)
RN  - 0 (Viral Envelope Proteins)
RN  - EC 3.2.1.25 (Hyal2 protein, human)
RN  - EC 3.2.1.35 (Hyaluronoglucosaminidase)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Antigens, CD/*metabolism
MH  - Betaretrovirus/metabolism
MH  - COS Cells
MH  - Cats
MH  - Cell Adhesion Molecules/*metabolism
MH  - Cell Line, Tumor
MH  - Chlorocebus aethiops
MH  - Dogs
MH  - Endogenous Retroviruses/*metabolism
MH  - GPI-Linked Proteins/metabolism
MH  - HEK293 Cells
MH  - HeLa Cells
MH  - Humans
MH  - Hyaluronoglucosaminidase/*metabolism
MH  - Jaagsiekte sheep retrovirus/metabolism
MH  - Mammary Tumor Virus, Mouse/metabolism
MH  - Mice
MH  - Receptors, Transferrin/*metabolism
MH  - Receptors, Virus
MH  - Vero Cells
MH  - Viral Envelope Proteins/*metabolism
MH  - Viral Tropism/*physiology
MH  - Virus Internalization
OTO - NOTNLM
OT  - Betaretrovirus
OT  - Envelope
OT  - HERV-K
OT  - Human endogenous retrovirus
OT  - Receptor
OT  - Tropism
OT  - Virus entry
EDAT- 2015/10/31 06:00
MHDA- 2016/04/26 06:00
CRDT- 2015/10/31 06:00
PHST- 2015/07/30 00:00 [received]
PHST- 2015/10/09 00:00 [revised]
PHST- 2015/10/14 00:00 [accepted]
PHST- 2015/10/31 06:00 [entrez]
PHST- 2015/10/31 06:00 [pubmed]
PHST- 2016/04/26 06:00 [medline]
AID - S0042-6822(15)00442-0 [pii]
AID - 10.1016/j.virol.2015.10.014 [doi]
PST - ppublish
SO  - Virology. 2016 Jan;487:121-8. doi: 10.1016/j.virol.2015.10.014. Epub 2015 Oct 27.