PMID- 26517624
OWN - NLM
STAT- MEDLINE
DCOM- 20160830
LR  - 20151120
IS  - 1465-3931 (Electronic)
IS  - 0031-3025 (Linking)
VI  - 47
IP  - 7
DP  - 2015 Dec
TI  - Reassessment of H&E stained clot specimens and immunohistochemistry of 
      phosphorylated Stat5 for histological diagnosis of MDS/MPN.
PG  - 673-7
LID - 10.1097/PAT.0000000000000328 [doi]
AB  - Few studies have comprehensively analysed histopathological findings of bone 
      marrow clots for diagnosis of haematopoietic cell dysplasia. In particular, a 
      limited number of studies have assessed the use of haematoxylin and eosin (H&E) 
      staining, which is generally considered less informative than May-Giemsa 
      staining. In the current study, the utility of bone marrow clot specimens for 
      diagnosis was examined using H&E staining and immunohistochemistry. Patients with 
      myelodysplastic syndromes (MDS) and myelodysplastic/myeloproliferative neoplasm 
      (MDS/MPN), including chronic myelomonocytic leukaemia (CMML), atypical chronic 
      myeloid leukaemia (aCML) lacking Philadelphia chromosome, and juvenile 
      myelomonocytic leukaemia (JMML), were selected for histological evaluation. H&E 
      stained specimens were advantageous for observation of atypical basophilic 
      staining of the cytoplasm and nucleus related to dysplasia. This finding was 
      significantly supported for both MDS and MDS/MPN (p < 0.05 versus May-Giemsa 
      staining); therefore, we concluded that H&E staining could be used for 
      identification of dysplastic cells. In addition, despite the loss of tissue 
      structure, phosphorylated Stat5 immunostaining was sufficiently useful for the 
      observation of myelodysplastic blasts. Thus, clot specimens are useful for 
      diagnosis of haematopoietic dysplasia by pathologists.
FAU - Tsuruyama, Tatsuaki
AU  - Tsuruyama T
AD  - 1Department of Pathology and Center for Anatomical, Pathological, and Forensic 
      Medical Research, Graduate School of Medicine, Kyoto University, 
      Yoshidakonoe-cho, Sakyo-ku, Kyoto 2Department of Pathology and Biology of 
      Diseases, Graduate School of Medicine, Kyoto University Hospital, 
      Yoshidakonoe-cho, Sakyo-ku, Kyoto, Japan.
FAU - Aini, Wulamujiang
AU  - Aini W
FAU - Hiratsuka, Takuya
AU  - Hiratsuka T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Pathology
JT  - Pathology
JID - 0175411
RN  - 0 (STAT5 Transcription Factor)
RN  - TDQ283MPCW (Eosine Yellowish-(YS))
RN  - YKM8PY2Z55 (Hematoxylin)
SB  - IM
MH  - Biopsy, Needle
MH  - Bone Marrow/pathology
MH  - Cytodiagnosis/*methods
MH  - Eosine Yellowish-(YS)
MH  - Hematoxylin
MH  - Humans
MH  - Immunohistochemistry
MH  - Myelodysplastic-Myeloproliferative Diseases/*diagnosis
MH  - Phosphorylation
MH  - STAT5 Transcription Factor/analysis/*biosynthesis
MH  - Staining and Labeling/*methods
EDAT- 2015/10/31 06:00
MHDA- 2016/08/31 06:00
CRDT- 2015/10/31 06:00
PHST- 2015/10/31 06:00 [entrez]
PHST- 2015/10/31 06:00 [pubmed]
PHST- 2016/08/31 06:00 [medline]
AID - 10.1097/PAT.0000000000000328 [doi]
PST - ppublish
SO  - Pathology. 2015 Dec;47(7):673-7. doi: 10.1097/PAT.0000000000000328.