PMID- 26518024
OWN - NLM
STAT- MEDLINE
DCOM- 20160808
LR  - 20191210
IS  - 1432-2072 (Electronic)
IS  - 0033-3158 (Linking)
VI  - 233
IP  - 2
DP  - 2016 Jan
TI  - M3 muscarinic receptor in the ventral medial prefrontal cortex modulating the 
      expression of contextual fear conditioning in rats.
PG  - 267-80
LID - 10.1007/s00213-015-4109-5 [doi]
AB  - RATIONALE: Basal forebrain cholinergic neurons modulate the activation of 
      cortical neurons by several stimuli such as fear and anxiety. However, the role 
      of the muscarinic receptor in the medial prefrontal cortex (MPFC) in the 
      modulation of the conditioned emotional response (CER) evoked in the model 
      contextual conditioned fear remains unclear. OBJECTIVES: The objective of this 
      study is to test the hypothesis that inhibition of the muscarinic receptor in 
      ventral MPFC modulates CER observed during animal's re-exposure to the aversive 
      context. METHODS: Rats implanted with cannulae aimed at the prelimbic (PL) or the 
      infralimbic (IL) were submitted to a high-intensity contextual fear conditioning 
      protocol. Before the test session, they received microinjections of the 
      hemicholinium (choline reuptake blocker), atropine (muscarinic antagonist), 
      J104129 fumarate (M1-M3 muscarinic antagonists), pirenzepine (M1 muscarinic 
      antagonist), neostigmine (inhibitor acetylcholinesterase enzyme), or the systemic 
      administration of the FG7142 (inverse benzodiazepine agonist). Additional 
      independent groups received the neostigmine or FG7142 before the ineffective 
      doses of J104129 fumarate in the low-intensity protocol of contextual fear 
      conditioning. RESULTS: In the high-intensity protocol, the administration of 
      hemicholinium (1 nmol), atropine (0.06-6 nmol), J104129 fumarate (6 nmol), or 
      pirenzepine (6 nmol) attenuated the expression of CER in rats. However, in the 
      low-intensity protocol, only J10129 fumarate (0.06 nmol) reduced the expression 
      of the CER. Finally, neostigmine (0.1-1 nmol) or FG7142 (8 mg/Kg) increased CER 
      expression, an effect inhibited by the low dose of the J10129 fumarate. 
      CONCLUSIONS: These results indicated that the blockade of M3 muscarinic receptor 
      in the vMPFC attenuates the CER expression.
FAU - Fedoce, A G
AU  - Fedoce AG
AD  - Department of Pharmacology, Ribeirao Preto School of Medicine, University of Sao 
      Paulo, Av. Bandeirantes, 3900, CEP: 14049-900, Ribeirao Preto, SP, Brazil.
AD  - Center for Interdisciplinary Research on Applied Neurosciences (NAPNA), 
      University of Sao Paulo, Sao Paulo, Brazil.
FAU - Ferreira-Junior, N C
AU  - Ferreira-Junior NC
AD  - Department of Pharmacology, Ribeirao Preto School of Medicine, University of Sao 
      Paulo, Av. Bandeirantes, 3900, CEP: 14049-900, Ribeirao Preto, SP, Brazil.
AD  - Center for Interdisciplinary Research on Applied Neurosciences (NAPNA), 
      University of Sao Paulo, Sao Paulo, Brazil.
FAU - Reis, D G
AU  - Reis DG
AD  - Department of Pharmacology, Ribeirao Preto School of Medicine, University of Sao 
      Paulo, Av. Bandeirantes, 3900, CEP: 14049-900, Ribeirao Preto, SP, Brazil.
AD  - Center for Interdisciplinary Research on Applied Neurosciences (NAPNA), 
      University of Sao Paulo, Sao Paulo, Brazil.
FAU - Correa, F M A
AU  - Correa FM
AD  - Department of Pharmacology, Ribeirao Preto School of Medicine, University of Sao 
      Paulo, Av. Bandeirantes, 3900, CEP: 14049-900, Ribeirao Preto, SP, Brazil.
FAU - Resstel, L B M
AU  - Resstel LB
AD  - Department of Pharmacology, Ribeirao Preto School of Medicine, University of Sao 
      Paulo, Av. Bandeirantes, 3900, CEP: 14049-900, Ribeirao Preto, SP, Brazil. 
      leoresstel@yahoo.com.br.
AD  - Center for Interdisciplinary Research on Applied Neurosciences (NAPNA), 
      University of Sao Paulo, Sao Paulo, Brazil. leoresstel@yahoo.com.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151031
PL  - Germany
TA  - Psychopharmacology (Berl)
JT  - Psychopharmacology
JID - 7608025
RN  - 0 (Cholinesterase Inhibitors)
RN  - 0 (Muscarinic Antagonists)
RN  - 0 (Receptor, Muscarinic M3)
RN  - 12794-10-4 (Benzodiazepines)
SB  - IM
MH  - Animals
MH  - Benzodiazepines/agonists
MH  - Cholinesterase Inhibitors/pharmacology
MH  - *Conditioning, Psychological/drug effects
MH  - Emotions
MH  - Fear/*psychology
MH  - Male
MH  - Muscarinic Antagonists/pharmacology
MH  - Parasympathetic Nervous System/drug effects
MH  - Prefrontal Cortex/drug effects/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, Muscarinic M3/drug effects/*metabolism
MH  - Synaptic Transmission/drug effects
OTO - NOTNLM
OT  - Acetylcholine
OT  - Anxiety
OT  - Conditioned emotional response
OT  - Medial prefrontal cortex
OT  - Muscarinic receptor
EDAT- 2015/11/01 06:00
MHDA- 2016/08/09 06:00
CRDT- 2015/11/01 06:00
PHST- 2015/03/25 00:00 [received]
PHST- 2015/10/03 00:00 [accepted]
PHST- 2015/11/01 06:00 [entrez]
PHST- 2015/11/01 06:00 [pubmed]
PHST- 2016/08/09 06:00 [medline]
AID - 10.1007/s00213-015-4109-5 [pii]
AID - 10.1007/s00213-015-4109-5 [doi]
PST - ppublish
SO  - Psychopharmacology (Berl). 2016 Jan;233(2):267-80. doi: 
      10.1007/s00213-015-4109-5. Epub 2015 Oct 31.