PMID- 26519760
OWN - NLM
STAT- MEDLINE
DCOM- 20160803
LR  - 20181202
IS  - 1095-6840 (Electronic)
IS  - 0016-6480 (Linking)
VI  - 225
DP  - 2016 Jan 1
TI  - Cloning, mRNA expression and transcriptional regulation of five retinoid X 
      receptor subtypes in yellow catfish Pelteobagrus fulvidraco by insulin.
PG  - 133-141
LID - S0016-6480(15)30008-3 [pii]
LID - 10.1016/j.ygcen.2015.10.010 [doi]
AB  - Retinoid X receptors (RXRs) are members of the nuclear receptor superfamily and 
      mediate development, reproduction, homeostasis and cell differentiation processes 
      in vertebrates. In this study, full-length cDNA sequences of five rxr subtypes 
      from yellow catfish Pelteobagrus fulvidraco were cloned. Their mRNA expression 
      patterns in different tissues and transcriptional regulation by insulin were 
      determined. Five P. fulvidraco rxr (Pf-rxr) subtypes differed in the length of 
      cDNA sequence and the open reading frame, but shared the similar domain 
      structures as in typical nuclear receptors. Phylogenetic analysis revealed that 
      the five Pf-rxr subtypes were paralogous genes, and that Pf-rxrbetaa and Pf-rxrbetab 
      had arisen during a teleost-specific genome duplication event. Five subtypes of 
      Pf-rxr were detected in all the tested tissues. Overlapping and distinct 
      expression patterns were found for different Pf-rxr subtypes, suggesting 
      functional redundancy and divergence of these duplicates. Intraperitoneal insulin 
      injection and incubation reduced the mRNA expression of Pf-rxrgb, but not other 
      subtypes, in the liver and hepatocytes of P. fulvidraco, respectively, suggesting 
      that Pf-rxrgb is the dominant rxr subtype involved in the insulin signaling 
      pathway in P. fulvidraco.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Pan, Ya-Xiong
AU  - Pan YX
AD  - Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., 
      Fishery College, Huazhong Agricultural University, Wuhan 430070, China; 
      Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan 
      430070, China.
FAU - Luo, Zhi
AU  - Luo Z
AD  - Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., 
      Fishery College, Huazhong Agricultural University, Wuhan 430070, China; 
      Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan 
      430070, China. Electronic address: luozhi99@mail.hzau.edu.cn.
FAU - Wu, Kun
AU  - Wu K
AD  - Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., 
      Fishery College, Huazhong Agricultural University, Wuhan 430070, China; 
      Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan 
      430070, China.
FAU - Zhang, Li-Han
AU  - Zhang LH
AD  - Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., 
      Fishery College, Huazhong Agricultural University, Wuhan 430070, China; 
      Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan 
      430070, China.
FAU - Xu, Yi-Huan
AU  - Xu YH
AD  - Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., 
      Fishery College, Huazhong Agricultural University, Wuhan 430070, China; 
      Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan 
      430070, China.
FAU - Chen, Qi-Liang
AU  - Chen QL
AD  - Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., 
      Fishery College, Huazhong Agricultural University, Wuhan 430070, China; 
      Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan 
      430070, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151111
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (Insulin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Retinoid X Receptors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Catfishes/genetics/*metabolism
MH  - Cloning, Molecular
MH  - Gene Expression Regulation/*drug effects
MH  - Hepatocytes/drug effects/metabolism
MH  - Insulin/*pharmacology
MH  - Liver/drug effects/metabolism
MH  - Phylogeny
MH  - RNA, Messenger/genetics
MH  - Receptors, Cytoplasmic and Nuclear/metabolism
MH  - Retinoid X Receptors/genetics/*metabolism
MH  - Transcription, Genetic/*drug effects
OTO - NOTNLM
OT  - Gene expression profiling
OT  - Insulin
OT  - Molecular cloning
OT  - Pelteobagrus fulvidraco
OT  - Retinoid X receptors
EDAT- 2015/11/01 06:00
MHDA- 2016/08/04 06:00
CRDT- 2015/11/01 06:00
PHST- 2015/07/02 00:00 [received]
PHST- 2015/10/07 00:00 [revised]
PHST- 2015/10/12 00:00 [accepted]
PHST- 2015/11/01 06:00 [entrez]
PHST- 2015/11/01 06:00 [pubmed]
PHST- 2016/08/04 06:00 [medline]
AID - S0016-6480(15)30008-3 [pii]
AID - 10.1016/j.ygcen.2015.10.010 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2016 Jan 1;225:133-141. doi: 10.1016/j.ygcen.2015.10.010. 
      Epub 2015 Nov 11.