PMID- 26521177
OWN - NLM
STAT- MEDLINE
DCOM- 20161013
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 408
IP  - 2
DP  - 2016 Jan
TI  - A nanoaggregate-on-mirror platform for molecular and biomolecular detection by 
      surface-enhanced Raman spectroscopy.
PG  - 609-18
LID - 10.1007/s00216-015-9142-z [doi]
AB  - A nanoaggregate-on-mirror (NAOM) structure has been developed for molecular and 
      biomolecular detection using surface-enhanced Raman spectroscopy (SERS). The 
      smooth surface of the gold mirror allows for simple and homogeneous 
      functionalization, while the introduction of the nanoaggregates enhances the 
      Raman signal of the molecule(s) in the vicinity of the aggregate-mirror junction. 
      This is evidenced by functionalizing the gold mirror with 4-nitrothiophenol, and 
      the further addition of gold nanoaggregates promotes local SERS activity only in 
      the areas with the nanoaggregates. The application of the NAOM platform for 
      biomolecular detection is highlighted using glucose and H2O2 as molecules of 
      interest. In both cases, the gold mirror is functionalized with 
      4-mercaptophenylboronic acid (4-MPBA). Upon exposure to glucose, the boronic acid 
      moiety of 4-MPBA forms a cyclic boronate ester. Once the nanoaggregates are added 
      to the surface, detection of glucose is possible without the use of an enzyme. 
      This method of indirect detection provides a limit of detection of 0.05 mM, along 
      with a linear range of detection from 0.1 to 15 mM for glucose, encompassing the 
      physiological range of blood glucose concentration. The detection of H2O2 is 
      achieved with optical inspection and SERS. The H2O2 interferes with the coating 
      of the gold mirror, enabling qualitative detection by visual inspection. 
      Simultaneously, the H2O2 reacts with the boronic acid to form a phenol, a change 
      that is detected by SERS.
FAU - Wallace, Gregory Q
AU  - Wallace GQ
AD  - Department of Chemistry, University of Western Ontario, 1151 Richmond St., 
      London, Ontario, N6A 5B7, Canada.
AD  - Centre for Advanced Materials and Biomaterials Research, University of Western 
      Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada.
FAU - Tabatabaei, Mohammadali
AU  - Tabatabaei M
AD  - Department of Chemistry, University of Western Ontario, 1151 Richmond St., 
      London, Ontario, N6A 5B7, Canada.
AD  - Centre for Advanced Materials and Biomaterials Research, University of Western 
      Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada.
FAU - Zuin, Mariachiara S
AU  - Zuin MS
AD  - Department of Chemistry, University of Western Ontario, 1151 Richmond St., 
      London, Ontario, N6A 5B7, Canada.
AD  - Centre for Advanced Materials and Biomaterials Research, University of Western 
      Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada.
FAU - Workentin, Mark S
AU  - Workentin MS
AD  - Department of Chemistry, University of Western Ontario, 1151 Richmond St., 
      London, Ontario, N6A 5B7, Canada.
AD  - Centre for Advanced Materials and Biomaterials Research, University of Western 
      Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada.
FAU - Lagugne-Labarthet, Francois
AU  - Lagugne-Labarthet F
AD  - Department of Chemistry, University of Western Ontario, 1151 Richmond St., 
      London, Ontario, N6A 5B7, Canada. flagugne@uwo.ca.
AD  - Centre for Advanced Materials and Biomaterials Research, University of Western 
      Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada. flagugne@uwo.ca.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151031
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Phenols)
RN  - 0 (Sulfhydryl Compounds)
RN  - 7440-57-5 (Gold)
RN  - 7K011JR4T0 (thiophenol)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Glucose/*analysis
MH  - Gold/chemistry
MH  - Humans
MH  - Hydrogen Peroxide/*analysis
MH  - Metal Nanoparticles/*chemistry
MH  - Phenols/chemistry
MH  - Spectrum Analysis, Raman/instrumentation/*methods
MH  - Sulfhydryl Compounds/chemistry
OTO - NOTNLM
OT  - 4-Mercaptophenylboronic acid
OT  - Glucose
OT  - Hydrogen peroxide
OT  - Nanoaggregate-on-mirror
OT  - Surface-enhanced Raman spectroscopy
EDAT- 2015/11/02 06:00
MHDA- 2016/10/14 06:00
CRDT- 2015/11/02 06:00
PHST- 2015/09/10 00:00 [received]
PHST- 2015/10/21 00:00 [accepted]
PHST- 2015/10/07 00:00 [revised]
PHST- 2015/11/02 06:00 [entrez]
PHST- 2015/11/02 06:00 [pubmed]
PHST- 2016/10/14 06:00 [medline]
AID - 10.1007/s00216-015-9142-z [pii]
AID - 10.1007/s00216-015-9142-z [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2016 Jan;408(2):609-18. doi: 10.1007/s00216-015-9142-z. Epub 
      2015 Oct 31.