PMID- 26521267
OWN - NLM
STAT- MEDLINE
DCOM- 20160407
LR  - 20181113
IS  - 1432-1904 (Electronic)
IS  - 0028-1042 (Linking)
VI  - 102
IP  - 11-12
DP  - 2015 Dec
TI  - Chemical composition and antimicrobial activity of honeybee (Apis mellifera 
      ligustica) propolis from subtropical eastern Australia.
PG  - 68
LID - 10.1007/s00114-015-1318-z [doi]
AB  - Propolis is a material manufactured by bees and contains beeswax, bee salivary 
      secretions and plant resins. Propolis preparations have been used for millennia 
      by humans in food, cosmetics and medicines due to its antibacterial effects. 
      Within the hive, propolis plays an important role in bees' health, with much of 
      its bioactivity largely dependent on the plant resins the bees select for its 
      production. Few chemical studies are available on the chemistry of propolis 
      produced by Australian honeybees (Apis mellifera, Apidae). This study aimed to 
      determine the chemical composition as well as in vitro antimicrobial effects of 
      propolis harvested from honeybees in subtropical eastern Australia. Honeybee 
      propolis was produced using plastic frames and multiple beehives in two 
      subtropical sites in eastern Australia. Methanolic extracts of propolis were 
      analysed by liquid chromatography with ultraviolet detection and high-resolution 
      mass spectrometry (ultra-high-pressure liquid chromatography 
      (UHPLC)-UV-high-resolution tandem mass spectrometry (HR-MS/MS)) and by gas 
      chromatography mass spectrometry (GC-MS). The resulting chemical data were 
      dereplicated for compound characterisation. The two crude extracts in abs. 
      ethanol were tested in vitro by the agar diffusion and broth dilution methods, 
      using a phenol standard solution as the positive control and abs. ethanol as the 
      negative control. Chemical constituents were identified to be pentacyclic 
      triterpenoids and C-prenylated flavonoids, including Abyssinoflavanone VII, 
      Propolin C and Nymphaeol C. The two propolis crude extracts showed bactericidal 
      effects at the minimal inhibitory concentrations of 0.37-2.04 mg mL(-1) against 
      Staphylococcus aureus ATCC 25923. However, the extracts were inactive against 
      Klebsiella pneumoniae ATCC 13883 and Candida albicans ATCC 10231. The 
      antistaphylococcal potential of propolis was discussed, also in relation to 
      honeybees' health, as it warrants further investigations on the social and 
      individual immunities of Australian honeybees.
FAU - Massaro, Carmelina Flavia
AU  - Massaro CF
AD  - Faculty of Science, Health, Education and Engineering, University of the Sunshine 
      Coast, Maroochydore, DC, Australia. cfmassaro@gmail.com.
FAU - Simpson, Jack Bruce
AU  - Simpson JB
AD  - Research School of Biology, Australian National University, Canberra, Australia.
FAU - Powell, Daniel
AU  - Powell D
AD  - Faculty of Science, Health, Education and Engineering, University of the Sunshine 
      Coast, Maroochydore, DC, Australia.
FAU - Brooks, Peter
AU  - Brooks P
AD  - Faculty of Science, Health, Education and Engineering, University of the Sunshine 
      Coast, Maroochydore, DC, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151031
PL  - Germany
TA  - Naturwissenschaften
JT  - Die Naturwissenschaften
JID - 0400767
RN  - 0 (Anti-Infective Agents)
RN  - 9009-62-5 (Propolis)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Animals
MH  - Anti-Infective Agents/chemistry/isolation & purification/pharmacology
MH  - Australia
MH  - Bees/*chemistry
MH  - Candida albicans/*drug effects
MH  - Chromatography, Liquid
MH  - Gas Chromatography-Mass Spectrometry
MH  - Klebsiella pneumoniae/*drug effects
MH  - Methanol/chemistry
MH  - Propolis/*chemistry/isolation & purification/*pharmacology
MH  - Staphylococcus aureus/*drug effects
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Candida albicans
OT  - Geranyl flavonoids
OT  - Klebsiella pneumoniae
OT  - Mass spectrometry
OT  - Staphylococcus aureus
OT  - Triterpenoids
EDAT- 2015/11/02 06:00
MHDA- 2016/04/08 06:00
CRDT- 2015/11/02 06:00
PHST- 2015/03/14 00:00 [received]
PHST- 2015/10/22 00:00 [accepted]
PHST- 2015/10/18 00:00 [revised]
PHST- 2015/11/02 06:00 [entrez]
PHST- 2015/11/02 06:00 [pubmed]
PHST- 2016/04/08 06:00 [medline]
AID - 10.1007/s00114-015-1318-z [pii]
AID - 10.1007/s00114-015-1318-z [doi]
PST - ppublish
SO  - Naturwissenschaften. 2015 Dec;102(11-12):68. doi: 10.1007/s00114-015-1318-z. Epub 
      2015 Oct 31.