PMID- 26521792
OWN - NLM
STAT- MEDLINE
DCOM- 20160523
LR  - 20181113
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 128
IP  - 21
DP  - 2015 Nov 5
TI  - Expression of Brain-derived Neurotrophic Factor and Tyrosine Kinase B in 
      Cerebellum of Poststroke Depression Rat Model.
PG  - 2926-31
LID - 10.4103/0366-6999.168058 [doi]
AB  - BACKGROUND: The pathophysiology of poststroke depression (PSD) remains elusive 
      because of its proposed multifactorial nature. Accumulating evidence suggests 
      that brain-derived neurotrophic factor (BDNF) plays a key role in the 
      pathophysiology of depression and PSD. And the cerebellar dysfunction may be 
      important in the etiology of depression; it is not clear whether it also has a 
      major effect on the risk of PSD. This study aimed to explore the expression of 
      BDNF and high-affinity receptors tyrosine kinase B (TrkB) in the cerebellum of 
      rats with PSD. METHODS: The rat models with focal cerebral ischemic were made 
      using a thread embolization method. PSD rat models were established with 
      comprehensive separate breeding and unpredicted chronic mild stress (UCMS) on 
      this basis. A normal control group, depression group, and a stroke group were 
      used to compare with the PSD group. Thirteen rats were used in each group. 
      Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) 
      for detecting the expression of BDNF and TrkB protein and mRNA in the cerebellum 
      were used at the 29 th day following the UCMS. RESULTS: Compared with the normal 
      control group and the stroke group, the number of BDNF immunoreactive (IR) 
      positive neurons was less in the PSD group (P < 0.05). Furthermore, the number of 
      TrkB IR positive cells was significantly less in the PSD group than that in the 
      normal control group (P < 0.05). The gene expression of BDNF and TrkB in the 
      cerebellum of PSD rats also decreased compared to the normal control group (P < 
      0.05). CONCLUSIONS: These findings suggested a possible association between 
      expression of BDNF and TrkB in the cerebellum and the pathogenesis of PSD.
FAU - Li, Yun
AU  - Li Y
AD  - Department of Neurology, The Affiliated Hospital of Dali University, Dali, Yunnan 
      671000, China.
FAU - Peng, Chun
AU  - Peng C
FAU - Guo, Xu
AU  - Guo X
FAU - You, Jun-Jie
AU  - You JJ
FAU - Yadav, Harishankar Prasad
AU  - Yadav HP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cerebellum/*metabolism
MH  - Depression/*etiology/*metabolism
MH  - Female
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stroke/*complications
PMC - PMC4756899
EDAT- 2015/11/03 06:00
MHDA- 2016/05/24 06:00
PMCR- 2015/11/05
CRDT- 2015/11/03 06:00
PHST- 2015/11/03 06:00 [entrez]
PHST- 2015/11/03 06:00 [pubmed]
PHST- 2016/05/24 06:00 [medline]
PHST- 2015/11/05 00:00 [pmc-release]
AID - ChinMedJ_2015_128_21_2926_168058 [pii]
AID - CMJ-128-2926 [pii]
AID - 10.4103/0366-6999.168058 [doi]
PST - ppublish
SO  - Chin Med J (Engl). 2015 Nov 5;128(21):2926-31. doi: 10.4103/0366-6999.168058.