PMID- 26521941
OWN - NLM
STAT- MEDLINE
DCOM- 20170124
LR  - 20190609
IS  - 1976-670X (Electronic)
IS  - 1976-6696 (Print)
IS  - 1976-6696 (Linking)
VI  - 49
IP  - 4
DP  - 2016 Apr
TI  - miR-185 inhibits endoplasmic reticulum stress-induced apoptosis by targeting 
      Na+/H+ exchanger-1 in the heart.
PG  - 208-13
AB  - Prolonged ER stress (ERS) can be associated with the induction of apoptotic cell 
      death in various heart diseases. In this study, we searched for microRNAs 
      affecting ERS in the heart using in silico and in vitro methods. We found that 
      miR-185 directly targets the 3'-untranslated region of Na+/H+ exchanger-1 
      (NHE-1), a protein involved in ERS. Cardiomyocyte ERS-triggered apoptosis induced 
      by 100 ng/ml tunicamycin (TM) or 1 muM thapsigargin (TG), ERS inducers, was 
      significantly reduced by miR-185 overexpression. Protein expression of 
      pro-apoptotic markers such as CCAAT/enhancer-binding protein homologous protein 
      (CHOP) and cleaved-caspase-3 was also markedly reduced by miR-185 in a 
      dose-dependent manner. Cariporide (20 muM), a pharmacological inhibitor of NHE-1, 
      also attenuated ERS-induced apoptosis in cardiomyocytes and CHOP protein 
      expression, suggesting that NHE-1 plays an important role in ERS-associated 
      apoptosis in cardiomyocytes. Collectively, the present results demonstrate that 
      miR-185 is involved in cardio- protection against ERS-mediated apoptotic cell 
      death. [BMB Reports 2016; 49(4): 208-213].
FAU - Kim, Jin Ock
AU  - Kim JO
AD  - School of Life Sciences and Systems Biology Research Center, Gwangju Institute of 
      Science and Technology (GIST), Gwangju 61005, Korea.
FAU - Kwon, Eun Jeong
AU  - Kwon EJ
AD  - School of Life Sciences and Systems Biology Research Center, Gwangju Institute of 
      Science and Technology (GIST), Gwangju 61005, Korea.
FAU - Song, Dong Woo
AU  - Song DW
AD  - School of Life Sciences and Systems Biology Research Center, Gwangju Institute of 
      Science and Technology (GIST), Gwangju 61005, Korea.
FAU - Lee, Jong Sub
AU  - Lee JS
AD  - School of Life Sciences and Systems Biology Research Center, Gwangju Institute of 
      Science and Technology (GIST), Gwangju 61005, Korea.
FAU - Kim, Do Han
AU  - Kim DH
AD  - School of Life Sciences and Systems Biology Research Center, Gwangju Institute of 
      Science and Technology (GIST), Gwangju 61005, Korea.
LA  - eng
PT  - News
PL  - Korea (South)
TA  - BMB Rep
JT  - BMB reports
JID - 101465334
RN  - 0 (3' Untranslated Regions)
RN  - 0 (MIRN185 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Slc9a1 protein, rat)
RN  - 0 (Sodium-Hydrogen Exchanger 1)
RN  - 0 (Sodium-Hydrogen Exchangers)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - Animals
MH  - *Apoptosis/genetics
MH  - Base Sequence
MH  - Binding Sites
MH  - *Endoplasmic Reticulum Stress
MH  - MicroRNAs/genetics/*metabolism
MH  - Myocardium/*metabolism
MH  - Myocytes, Cardiac/cytology/metabolism
MH  - Protein Binding
MH  - Rats
MH  - Sodium-Hydrogen Exchanger 1
MH  - Sodium-Hydrogen Exchangers/antagonists & inhibitors/genetics/*metabolism
PMC - PMC4915239
EDAT- 2015/11/03 06:00
MHDA- 2017/01/25 06:00
PMCR- 2016/05/30
CRDT- 2015/11/03 06:00
PHST- 2015/09/24 00:00 [received]
PHST- 2015/11/03 06:00 [entrez]
PHST- 2015/11/03 06:00 [pubmed]
PHST- 2017/01/25 06:00 [medline]
PHST- 2016/05/30 00:00 [pmc-release]
AID - 3347 [pii]
AID - BMB-49-208 [pii]
AID - 10.5483/bmbrep.2016.49.4.193 [doi]
PST - ppublish
SO  - BMB Rep. 2016 Apr;49(4):208-13. doi: 10.5483/bmbrep.2016.49.4.193.