PMID- 26522116
OWN - NLM
STAT- MEDLINE
DCOM- 20160830
LR  - 20151102
IS  - 1875-6263 (Electronic)
IS  - 1028-4559 (Linking)
VI  - 54
IP  - 5
DP  - 2015 Oct
TI  - Interphase fluorescence in situ hybridization assisting in prenatal counseling 
      for amniocentesis karyotyping-detected fetal mosaicism.
PG  - 588-91
LID - S1028-4559(15)00177-1 [pii]
LID - 10.1016/j.tjog.2015.08.019 [doi]
AB  - OBJECTIVE: To evaluate how interphase fluorescence in situ hybridization (FISH) 
      played a role in genetic counseling when encountering prenatally detected fetal 
      mosaicism cases. MATERIALS AND METHODS: We retrospectively reviewed 17 cases of 
      amniotic fluid specimens diagnosed with Level III chromosome mosaicism using in 
      situ coverslip culture method. Among them, seven received additional interphase 
      FISH tests; five were related to autosomal mosaicism and two others were due to 
      sex chromosomes. RESULTS: In the autosome group, one couple chose to terminate 
      the pregnancy due to a high percentage of trisomy 21 cells (48.1%) shown on 
      interphase FISH; in the gonosome group, one case chose termination as FISH 
      exhibited as high as 80% of XXYY cells. CONCLUSION: Performing interphase FISH on 
      uncultured amniocytes for cases detected with mosaicism by traditional amniotic 
      fluid culture provided quick confirmation of the karyotyping results; 
      additionally, obtaining information about the extent of the abnormality involved 
      using interphase FISH could also play a role in counseling patients on the 
      decision making concerning the future of their pregnancies.
CI  - Copyright (c) 2015. Published by Elsevier B.V.
FAU - Su, Sheng-Yuan
AU  - Su SY
AD  - Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Lin-Kou 
      Medical Center, Chang Gung University, Taoyuan, Taiwan.
FAU - Chueh, Ho-Yen
AU  - Chueh HY
AD  - Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Lin-Kou 
      Medical Center, Chang Gung University, Taoyuan, Taiwan.
FAU - Li, Ching-Pei
AU  - Li CP
AD  - Cytogenetic Laboratory, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan.
FAU - Chang, Yao-Lung
AU  - Chang YL
AD  - Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Lin-Kou 
      Medical Center, Chang Gung University, Taoyuan, Taiwan.
FAU - Chang, Shuenn-Dyh
AU  - Chang SD
AD  - Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Lin-Kou 
      Medical Center, Chang Gung University, Taoyuan, Taiwan. Electronic address: 
      gene@cgmh.org.tw.
FAU - Chen, Chih-Ping
AU  - Chen CP
AD  - Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, 
      Taiwan; Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan; 
      Department of Biotechnology, Asia University, Taichung, Taiwan; School of Chinese 
      Medicine, College of Chinese Medicine, China Medical University, Taichung, 
      Taiwan; Institute of Clinical and Community Health Nursing, National Yang-Ming 
      University, Taipei, Taiwan; Department of Obstetrics and Gynecology, School of 
      Medicine, National Yang-Ming University, Taipei, Taiwan.
LA  - eng
PT  - Journal Article
PL  - China (Republic : 1949- )
TA  - Taiwan J Obstet Gynecol
JT  - Taiwanese journal of obstetrics & gynecology
JID - 101213819
SB  - IM
MH  - Adult
MH  - Amniocentesis/*methods
MH  - Amniotic Fluid/*cytology
MH  - Comparative Genomic Hybridization
MH  - Female
MH  - Genetic Counseling/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Karyotyping/methods
MH  - *Mosaicism
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Retrospective Studies
MH  - Trisomy/*diagnosis/genetics
OTO - NOTNLM
OT  - counseling
OT  - interphase fluorescence in situ hybridization
OT  - karyotype
OT  - mosaicism
EDAT- 2015/11/03 06:00
MHDA- 2016/08/31 06:00
CRDT- 2015/11/03 06:00
PHST- 2015/07/08 00:00 [received]
PHST- 2015/07/08 00:00 [accepted]
PHST- 2015/11/03 06:00 [entrez]
PHST- 2015/11/03 06:00 [pubmed]
PHST- 2016/08/31 06:00 [medline]
AID - S1028-4559(15)00177-1 [pii]
AID - 10.1016/j.tjog.2015.08.019 [doi]
PST - ppublish
SO  - Taiwan J Obstet Gynecol. 2015 Oct;54(5):588-91. doi: 10.1016/j.tjog.2015.08.019.