PMID- 26523221
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151102
LR  - 20200930
IS  - 2008-3866 (Print)
IS  - 2008-3874 (Electronic)
IS  - 2008-3866 (Linking)
VI  - 18
IP  - 9
DP  - 2015 Sep
TI  - Presentation of a novel model of chitosan- polyethylene oxide-nanohydroxyapatite 
      nanofibers together with bone marrow stromal cells to repair and improve minor 
      bone defects.
PG  - 887-93
AB  - OBJECTIVES: Various methods for repairing bone defects are presented. Cell 
      therapy is one of these methods. Bone marrow stromal cells (BMSCs) seem to be 
      suitable for this purpose. On the other hand, lots of biomaterials are used to 
      improve and repair the defect in the body, so in this study we tried to produce a 
      similar structure to the bone by the chitosan and hydroxyapatite. MATERIALS AND 
      METHODS: In this study, the solution of chitosan-nanohydroxyapatite-polyethylene 
      oxide (PEO) Nanofibers was produced by electrospinning method, and then the BMSCs 
      were cultured on this solution. A piece of chitosan-nanohydroxyapatite Nanofibers 
      with BMSCs was placed in a hole with the diameter of 1 mm at the distal epiphysis 
      of the rat femur. Then the biomechanical and radiographic studies were performed. 
      RESULTS: Biomechanical testing results showed that bone strength was 
      significantly higher in the Nanofiber/BMSCs group in comparison with control 
      group. Also the bone strength in nanofiber/BMSCs group was significant, but in 
      nanofiber group was nearly significant. Radiographic studies also showed that the 
      average amount of callus formation (radio opacity) in nanofiber and control group 
      was not significantly different. The callus formation in nanofiber/BMSCs group 
      was increased compared to the control group, and it was not significant in the 
      nanofiber group. CONCLUSION: Since chitosan-nanohydroxyapatite nanofibers with 
      BMSCs increases the rate of bone repair, the obtained cell-nanoscaffold shell can 
      be used in tissue engineering and cell therapy, especially for bone defects.
FAU - Emamgholi, Asgar
AU  - Emamgholi A
AD  - Neuroscience Research Center, Baqiyatallah University of Medical Science, Tehran, 
      Iran ; Microbial Biotechnology, Faculty of Science, Payame Noor University, Unit 
      of Tehran Center, Tehran, Iran.
FAU - Rahimi, Mohsen
AU  - Rahimi M
AD  - Department of Parasitology and Mycology, School of Medicine, Baqiyatallah 
      University of Medical Science, Tehran, Iran.
FAU - Kaka, Gholamreza
AU  - Kaka G
AD  - Neuroscience Research Center, Baqiyatallah University of Medical Science, Tehran, 
      Iran.
FAU - Sadraie, Seyed Homayoon
AU  - Sadraie SH
AD  - Department of Anatomy, School of Medicine, Baqiyatallah University of Medical 
      Science, Tehran, Iran.
FAU - Najafi, Saleh
AU  - Najafi S
AD  - Microbial Biotechnology, Faculty of Science, Payame Noor University, Unit of 
      Tehran Center, Tehran, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Basic Med Sci
JT  - Iranian journal of basic medical sciences
JID - 101517966
PMC - PMC4620188
OTO - NOTNLM
OT  - BMSCs
OT  - Chitosan
OT  - Nanofibers
OT  - Scaffolds
EDAT- 2015/11/03 06:00
MHDA- 2015/11/03 06:01
PMCR- 2015/09/01
CRDT- 2015/11/03 06:00
PHST- 2015/11/03 06:00 [entrez]
PHST- 2015/11/03 06:00 [pubmed]
PHST- 2015/11/03 06:01 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
AID - IJBMS-18-887 [pii]
PST - ppublish
SO  - Iran J Basic Med Sci. 2015 Sep;18(9):887-93.