PMID- 26523509
OWN - NLM
STAT- MEDLINE
DCOM- 20161006
LR  - 20191210
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 419
DP  - 2016 Jan 5
TI  - C1q/TNF-related protein-9 inhibits cytokine-induced vascular inflammation and 
      leukocyte adhesiveness via AMP-activated protein kinase activation in endothelial 
      cells.
PG  - 235-43
LID - S0303-7207(15)30123-4 [pii]
LID - 10.1016/j.mce.2015.10.023 [doi]
AB  - Although recent studies have reported cardioprotective effects of C1q/TNF-related 
      protein 9 (CTRP9), the closet adiponectin paralog, its role on cytokine-induced 
      endothelial inflammation is unknown. We investigated whether CTRP9 prevented 
      inflammatory cytokine-induced nuclear factor-kappa B (NF-kappaB) activation and 
      inhibited the expression of adhesion molecules and a chemokine in the vascular 
      endothelial cell. We used human aortic endothelial cells (HAECs) to examine the 
      effects of CTRP9 on NF-kappaB activation and the expression of NF-kappaB-mediated genes, 
      including intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion 
      molecule-1 (VCAM-1), and monocyte chemoattractant protein-1 (MCP-1). Tumor 
      necrosis factor alpha (TNFalpha) was used as a representative proinflammatory 
      cytokine. In an adhesion assay using THP-1 cells, CTRP9 reduced TNFalpha-induced 
      adhesion of monocytes to HAECs. Treatment with CTRP9 significantly decreased 
      TNFalpha-induced activation of NF-kappaB, as well as the expression of ICAM-1, VCAM-1, 
      and MCP-1. In addition, treatment with CTRP9 significantly increased the 
      phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase 
      (ACC), the downstream target of AMPK. The inhibitory effect of CTRP9 on the 
      expression of ICAM-1, VCAM-1, and MCP-1 and monocyte adhesion to HAECs was 
      abolished after transfection with an AMPKalpha1-specific siRNA. Our study is the 
      first to demonstrate that CTRP9 attenuates cytokine-induced vascular inflammation 
      in endothelial cells mediated by AMPK activation.
CI  - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved.
FAU - Jung, Chang Hee
AU  - Jung CH
AD  - Department of Internal Medicine, University of Ulsan College of Medicine, Seoul, 
      Republic of Korea.
FAU - Lee, Min Jung
AU  - Lee MJ
AD  - Department of Internal Medicine, University of Ulsan College of Medicine, Seoul, 
      Republic of Korea.
FAU - Kang, Yu Mi
AU  - Kang YM
AD  - Department of Internal Medicine, University of Ulsan College of Medicine, Seoul, 
      Republic of Korea.
FAU - Lee, Yoo La
AU  - Lee YL
AD  - Asan Institute of Life Sciences, University of Ulsan College of Medicine, Seoul, 
      Republic of Korea.
FAU - Seol, So Mi
AU  - Seol SM
AD  - Asan Institute of Life Sciences, University of Ulsan College of Medicine, Seoul, 
      Republic of Korea.
FAU - Yoon, Hae Kyeong
AU  - Yoon HK
AD  - Asan Institute of Life Sciences, University of Ulsan College of Medicine, Seoul, 
      Republic of Korea.
FAU - Kang, Sang-Wook
AU  - Kang SW
AD  - Department of Biomedical Sciences, University of Ulsan College of Medicine, 
      Seoul, Republic of Korea.
FAU - Lee, Woo Je
AU  - Lee WJ
AD  - Department of Internal Medicine, University of Ulsan College of Medicine, Seoul, 
      Republic of Korea.
FAU - Park, Joong-Yeol
AU  - Park JY
AD  - Department of Internal Medicine, University of Ulsan College of Medicine, Seoul, 
      Republic of Korea. Electronic address: jypark@amc.seoul.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151031
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Adiponectin)
RN  - 0 (C1QTNF9B protein, human)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Glycoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor Receptor-Associated Peptides and Proteins)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Adiponectin/*pharmacology
MH  - Aorta/cytology/drug effects/metabolism
MH  - Cell Line, Tumor
MH  - Chemokine CCL2/metabolism
MH  - Cytokines/*metabolism
MH  - Endothelial Cells/cytology/*drug effects/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Glycoproteins/*pharmacology
MH  - Humans
MH  - NF-kappa B/*pharmacology
MH  - Phosphorylation/drug effects
MH  - Signal Transduction
MH  - Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
MH  - Vascular Cell Adhesion Molecule-1/metabolism
OTO - NOTNLM
OT  - AMPK
OT  - Adhesion molecules
OT  - CTRP9
OT  - Endothelial cells
OT  - NF-kappaB
EDAT- 2015/11/03 06:00
MHDA- 2016/10/08 06:00
CRDT- 2015/11/03 06:00
PHST- 2015/06/24 00:00 [received]
PHST- 2015/10/02 00:00 [revised]
PHST- 2015/10/25 00:00 [accepted]
PHST- 2015/11/03 06:00 [entrez]
PHST- 2015/11/03 06:00 [pubmed]
PHST- 2016/10/08 06:00 [medline]
AID - S0303-7207(15)30123-4 [pii]
AID - 10.1016/j.mce.2015.10.023 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2016 Jan 5;419:235-43. doi: 10.1016/j.mce.2015.10.023. Epub 
      2015 Oct 31.