PMID- 26524137 OWN - NLM STAT- MEDLINE DCOM- 20160930 LR - 20220408 IS - 1873-2747 (Electronic) IS - 0361-9230 (Linking) VI - 120 DP - 2016 Jan TI - Electro-acupuncture at LI11 and ST36 acupoints exerts neuroprotective effects via reactive astrocyte proliferation after ischemia and reperfusion injury in rats. PG - 14-24 LID - S0361-9230(15)30050-2 [pii] LID - 10.1016/j.brainresbull.2015.10.011 [doi] AB - Reactive astrogliosis is a common phenomenon in central nervous system (CNS) injuries such as ischemic stroke. The present study aimed to deeply investigate the relationships between the neuroprotective effect of electro-acupuncture (EA) and reactive astrocytes following cerebral ischemia. EA treatment at the Quchi (LI11) and Zusanli (ST36) acupoints at Day 3 attenuated neurological deficits and cerebral infarct volume in ischemia and reperfusion (I/R) injured rats. Animal behavior assessments found that the speed of Catwalk gait, equilibrium and coordination of Rotarod test were improved. Furthermore, EA treatment exerted neuroprotective effects via activation of glial fibrillary acidic protein (GFAP), vimentin and nestin positive cells. Simultaneously, an obvious increase in GFAP/vimentin, GFAP/nestin and GFAP/BrdU co-labeling appeared in the peri-infract cortex and striatum, suggesting EA can promote the proliferation of GFAP/vimentin/nestin-positive reactive astrocytes. The expression of cell cycle-associated proteins Cyclin Dl, CDK4 and phospho-Rb were increased in the peri-infract cortex and striatum, indicating proliferated reactive astrocytes-mediated CyclinDl/CDK4 regulation of the transition of the G1-to-S cell cycle phases. In addition, EA enhanced the localized expression of brain-derived neurotrophic factor (BDNF) in the peri-infract cortex and striatum. These results demonstrated that EA treatment at the LI11 and ST36 acupoints on Day 3 exerted neuroprotection via proliferation of GFAP/vimentin/nestin-positive reactive astrocytes and, potentially, secretion of reactive astrocytes-derived BDNF in I/R injured rats. CI - Copyright (c) 2015 Elsevier Inc. All rights reserved. FAU - Tao, Jing AU - Tao J AD - College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. FAU - Zheng, Yi AU - Zheng Y AD - The Fujian Province Key Laboratory of Rehabilitation Technology, Fuzhou 350001, China. FAU - Liu, Weilin AU - Liu W AD - College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. FAU - Yang, Shanli AU - Yang S AD - Fujian University of Traditional Chinese Medicine Affiliated Rehabilitation Hospital, Fuzhou 350001, China. FAU - Huang, Jia AU - Huang J AD - College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. FAU - Xue, Xiehua AU - Xue X AD - Fujian University of Traditional Chinese Medicine Affiliated Rehabilitation Hospital, Fuzhou 350001, China. FAU - Shang, Guanhao AU - Shang G AD - The Fujian Province Key Laboratory of Rehabilitation Technology, Fuzhou 350001, China. FAU - Wang, Xian AU - Wang X AD - The Fujian Province Key Laboratory of Rehabilitation Technology, Fuzhou 350001, China. FAU - Lin, Ruhui AU - Lin R AD - The Fujian Province Key Laboratory of Rehabilitation Technology, Fuzhou 350001, China. FAU - Chen, Lidian AU - Chen L AD - College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. Electronic address: cld@fjtcm.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20151030 PL - United States TA - Brain Res Bull JT - Brain research bulletin JID - 7605818 RN - 0 (Glial Fibrillary Acidic Protein) SB - IM MH - Acupuncture Points MH - Animals MH - Astrocytes/pathology/*physiology MH - Brain/pathology/*physiopathology MH - Brain Ischemia/pathology/physiopathology/*therapy MH - Cell Proliferation MH - Disease Models, Animal MH - Electroacupuncture/*methods MH - Glial Fibrillary Acidic Protein/metabolism MH - Infarction, Middle Cerebral Artery MH - Male MH - Motor Activity/physiology MH - Random Allocation MH - Rats, Sprague-Dawley MH - Reperfusion Injury/pathology/physiopathology/*therapy MH - Rotarod Performance Test OTO - NOTNLM OT - BDNF OT - Cell cycle OT - Electro-acupuncture OT - Ischemic stroke OT - Reactive astrocytes EDAT- 2015/11/03 06:00 MHDA- 2016/10/01 06:00 CRDT- 2015/11/03 06:00 PHST- 2015/08/09 00:00 [received] PHST- 2015/10/22 00:00 [revised] PHST- 2015/10/26 00:00 [accepted] PHST- 2015/11/03 06:00 [entrez] PHST- 2015/11/03 06:00 [pubmed] PHST- 2016/10/01 06:00 [medline] AID - S0361-9230(15)30050-2 [pii] AID - 10.1016/j.brainresbull.2015.10.011 [doi] PST - ppublish SO - Brain Res Bull. 2016 Jan;120:14-24. doi: 10.1016/j.brainresbull.2015.10.011. Epub 2015 Oct 30.