PMID- 26524156
OWN - NLM
STAT- MEDLINE
DCOM- 20160229
LR  - 20151121
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 168
IP  - 1
DP  - 2015
TI  - In vitro Diagnosis of Immediate Drug Hypersensitivity: Should We Go with the 
      Flow.
PG  - 3-12
LID - 10.1159/000440663 [doi]
AB  - BACKGROUND: Diagnosis of immediate drug hypersensitivity reactions (IDHRs) is 
      based upon history taking, skin prick or intradermal tests and quantification of 
      specific immunoglobulin E (IgE) antibodies. Unfortunately, this is often 
      insufficient to correctly identify patients with IgE-mediated IDHRs and is 
      impossible in the case of non-IgE-mediated IDHRs. Drug provocation tests (DPT) 
      are considered the 'gold standard' diagnostic but are not always possible, for 
      ethical and practical reasons. Therefore, the validation of new cellular tests 
      such as basophil activation testing (BAT) was necessary. This review focuses on 
      the applications of BAT in IDHRs. METHODS: A literature search was conducted, 
      using the words basophil, flow cytometry, immediate drug allergy and drugs; this 
      was complemented by the authors' own expertise. RESULTS: BAT/HistaFlow(R) is a 
      useful diagnostic tool in IDHRs, mainly used to diagnose allergy to neuromuscular 
      blocking agents (NMBAs), antibiotics, nonsteroidal anti-inflammatory drugs 
      (NSAIDs) and iodinated radiocontrast media. Its sensitivity varies between 50 and 
      60%, and specificity attains 80%, except for with quinolones and NSAIDs. 
      CONCLUSIONS: The diagnostic utility of BAT (and to lesser extent HistaFlow) has 
      been demonstrated and is mostly applied in IDHRs. However, larger-scale 
      collaborative studies are necessary to optimize test protocols and validate the 
      entry of BAT as a diagnostic instrument in drug allergy.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - Mangodt, Evelyne A
AU  - Mangodt EA
AD  - Department of Immunology-Allergology-Rheumatology, Faculty of Medicine and Health 
      Science, University of Antwerp, Antwerp University Hospital, Antwerp, Belgium.
FAU - Van Gasse, Athina L
AU  - Van Gasse AL
FAU - Decuyper, Ine
AU  - Decuyper I
FAU - Uyttebroek, Astrid
AU  - Uyttebroek A
FAU - Faber, Margaretha A
AU  - Faber MA
FAU - Sabato, Vito
AU  - Sabato V
FAU - Bridts, Chris H
AU  - Bridts CH
FAU - Hagendorens, Margo M
AU  - Hagendorens MM
FAU - Ebo, Didier G
AU  - Ebo DG
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20151103
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
SB  - IM
MH  - Anti-Inflammatory Agents, Non-Steroidal/immunology
MH  - Basophils/immunology
MH  - Drug Hypersensitivity/*diagnosis/immunology
MH  - Humans
MH  - Hypersensitivity, Immediate/*diagnosis/immunology
EDAT- 2015/11/03 06:00
MHDA- 2016/03/02 06:00
CRDT- 2015/11/03 06:00
PHST- 2015/11/03 06:00 [entrez]
PHST- 2015/11/03 06:00 [pubmed]
PHST- 2016/03/02 06:00 [medline]
AID - 000440663 [pii]
AID - 10.1159/000440663 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2015;168(1):3-12. doi: 10.1159/000440663. Epub 2015 Nov 
      3.