PMID- 26524639
OWN - NLM
STAT- MEDLINE
DCOM- 20161114
LR  - 20161230
IS  - 1532-4281 (Electronic)
IS  - 1079-9893 (Linking)
VI  - 36
IP  - 2
DP  - 2016
TI  - GPR39 activates proliferation and differentiation of porcine intramuscular 
      preadipocytes through targeting the PI3K/AKT cell signaling pathway.
PG  - 130-8
LID - 10.3109/10799893.2015.1056308 [doi]
AB  - BACKGROUND: The orphan G protein-coupled receptor (GPR) 39 was originally 
      identified as the receptor of obestatin. In this study, the effects and 
      mechanisms of GPR39 on cell proliferation and differentiation were investigated 
      in cultured porcine intramuscular preadipocytes. METHODS: Morphology of 
      preadipocytes and accumulated lipid droplets within cells were identified by an 
      inverted microscope. After transfected with constructed pCMV-GPR39 plasmid, cell 
      proliferation was measured by using methyl thiazolyl tetrazolium method, mRNA 
      expression of GPR39, CCAAT/enhancer binding protein-alpha (C/EBPalpha), peroxisome 
      proliferator-activated receptor-gamma (PPARgamma), Caspase-9 and adipocyte determination 
      and differentiation factor-1 (ADD1) was determined by RNA preparation and reverse 
      transcription polymerase chain reaction, protein expression of phosphoinositide-3 
      kinase (PI3K), 3-phosphoinositide-dependent protein kinase 1, phosphorylated 
      glycogen synthase kinase 3 (pGSK3), total Akt and phosphorylated Akt (pAkt) was 
      analyzed by Western blot. RESULTS: It found that GPR39 mRNA and protein were 
      expressed in porcine intramuscular preadipocytes and its expression was 
      significantly up-regulated after treatment with Zn(2+) whose function is found to 
      be mediated by GPR39. Furthermore, over-expression of GPR39 further promoted the 
      optical density value of cells, enhanced mRNA expression of PPARgamma, C/EBPalpha and 
      ADD1, and inhibited mRNA expression of Caspase-9. Protein expression of pGSK3 and 
      pAkt was also increased by GPR39 stimulation. In addition, GPR39-induced 
      proliferation and differentiation of porcine intramuscular preadipocytes was 
      partially blocked by the Akt inhibitor (PDTC) and the PI3K inhibitor (LY294002). 
      CONCLUSION: It indicated that GPR39 was a transducer of Zn(2+), and enhanced 
      proliferation and differentiation of porcine intramuscular preadipocytes through 
      activation of the PI3K/Akt signaling pathway.
FAU - Dong, Xiaoying
AU  - Dong X
AD  - a College of Yingdong Agricultural Science and Engineering, Shaoguan University , 
      Shaoguan , P.R. China .
FAU - Tang, Shengqiu
AU  - Tang S
AD  - a College of Yingdong Agricultural Science and Engineering, Shaoguan University , 
      Shaoguan , P.R. China .
FAU - Zhang, Wei
AU  - Zhang W
AD  - b Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Hubei Academy of 
      Agricultural Science , Wuhan , P.R. China .
FAU - Gao, Weihua
AU  - Gao W
AD  - c College of Animal Science, Yangtze Univeisity , Jingzhou , P.R. China , and.
FAU - Chen, Yanfei
AU  - Chen Y
AD  - d College of Yingdong Life Science, Shaoguan University , Shaoguan , P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151102
PL  - England
TA  - J Recept Signal Transduct Res
JT  - Journal of receptor and signal transduction research
JID - 9509432
RN  - 0 (CCAAT-Enhancer-Binding Protein-alpha)
RN  - 0 (PPAR gamma)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Sterol Regulatory Element Binding Protein 1)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.4.22.- (Caspase 9)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Adipocytes/drug effects/*metabolism
MH  - Animals
MH  - CCAAT-Enhancer-Binding Protein-alpha/biosynthesis/genetics
MH  - Caspase 9/biosynthesis
MH  - Cell Differentiation/genetics
MH  - Cell Proliferation/genetics
MH  - Lipid Droplets/drug effects
MH  - PPAR gamma/biosynthesis/genetics
MH  - Phosphatidylinositol 3-Kinases/genetics
MH  - Proto-Oncogene Proteins c-akt/genetics
MH  - RNA, Messenger/biosynthesis
MH  - Receptors, G-Protein-Coupled/*biosynthesis/genetics
MH  - Signal Transduction/drug effects
MH  - Sterol Regulatory Element Binding Protein 1/biosynthesis/genetics
MH  - Swine
MH  - Zinc/*pharmacology
OTO - NOTNLM
OT  - Akt
OT  - GPR39
OT  - PI3K
OT  - differentiation
OT  - inhibitor
OT  - preadipocyte
OT  - proliferation
EDAT- 2015/11/03 06:00
MHDA- 2016/11/15 06:00
CRDT- 2015/11/03 06:00
PHST- 2015/11/03 06:00 [entrez]
PHST- 2015/11/03 06:00 [pubmed]
PHST- 2016/11/15 06:00 [medline]
AID - 10.3109/10799893.2015.1056308 [doi]
PST - ppublish
SO  - J Recept Signal Transduct Res. 2016;36(2):130-8. doi: 
      10.3109/10799893.2015.1056308. Epub 2015 Nov 2.