PMID- 26524887
OWN - NLM
STAT- MEDLINE
DCOM- 20160211
LR  - 20151103
IS  - 0047-1860 (Print)
IS  - 0047-1860 (Linking)
VI  - 63
IP  - 1
DP  - 2015 Jan
TI  - [Sample Quality Control through the Depository and Distribution of Cancer-Related 
      Human Materials: Experience of Kanagawa Cancer Research & Information Association 
      (KCRIA)].
PG  - 111-8
AB  - Kanagawa Prefecture and its department of hospital business established the 
      Kanagawa Cancer Research and Information Association (KCRIA) in 2006, and the 
      Kanagawa Cancer Center (KCC) and related institutes have started to collect and 
      distribute patient-derived cancer-related biomaterials to researchers. The tumor 
      tissue center of KCC is collecting frozen tumor tissues, formalin-fixed and 
      paraffin-embedded (FFPE) tumor tissues, serum of patients before treatment, and 
      genomic DNA of peripheral white blood cells. Corresponding normal tissues are 
      also collected if possible. Clinical information on patients is annotated in each 
      sample as accurately as possible under linkable anonymization to follow the 
      prognosis. We are evaluating the quality of tumor samples with two indices: the 
      RNA Integrity Number (RIN) of extracted total RNAs from randomly chosen frozen 
      tissues examined with the 2100 Bioanalyzer (Agilent Technologies, Inc., Santa 
      Clara, CA, USA), and the histopathological examination of parts of all frozen 
      tissues prepared in the Optimal Cutting Temperature (OTC) compound and evaluation 
      of the contents of tumor cells. Management of the sample quality is an important 
      issue for any biomaterial repository. The quality of samples is influenced by the 
      procedures of sample collection and storage, and also by the storage duration. 
      The quality could be periodically examined by monitoring analyte stability and 
      integrity, such as RIN of frozen cancer tissues. However, the best way to collect 
      and store RNA might not always be the same for different analytes. Therefore, 
      sample quality control at biomaterial repositories that are preparing samples for 
      future studies on undefined analytes is a very difficult problem. At least the 
      standard sample operation procedure (SOP) should be available for users.
FAU - Miyagi, Yohei
AU  - Miyagi Y
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Rinsho Byori
JT  - Rinsho byori. The Japanese journal of clinical pathology
JID - 2984781R
SB  - IM
MH  - *Clinical Laboratory Techniques
MH  - Cryopreservation
MH  - Japan
MH  - Neoplasms/*pathology
MH  - *Quality Control
MH  - *Specimen Handling/standards
MH  - Time Factors
MH  - *Tissue Preservation
EDAT- 2015/11/04 06:00
MHDA- 2016/02/13 06:00
CRDT- 2015/11/04 06:00
PHST- 2015/11/04 06:00 [entrez]
PHST- 2015/11/04 06:00 [pubmed]
PHST- 2016/02/13 06:00 [medline]
PST - ppublish
SO  - Rinsho Byori. 2015 Jan;63(1):111-8.