PMID- 26525345
OWN - NLM
STAT- MEDLINE
DCOM- 20160815
LR  - 20181113
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2015
DP  - 2015
TI  - Kinetic Profiles of Inflammatory Mediators in the Conjunctival Sac Fluid of 
      Patients upon Photorefractive Keratectomy.
PG  - 942948
LID - 10.1155/2015/942948 [doi]
LID - 942948
AB  - Photorefractive keratectomy (PRK) represents a therapeutic option to remodel 
      corneal stroma and to compensate refractive errors, which involves inflammatory 
      and/or regenerative processes. In this context, the modulation of 
      cytokines/chemokines in the conjunctival sac fluid and their role in the 
      maintenance of the corneal microenvironment during the healing process upon 
      refractive procedures has not been deeply investigated. In this study, serial 
      samples of conjunctival sac fluid of patients (n = 25) undergoing PRK were 
      harvested before and at different time points after surgery. The levels of 29 
      cytokines/chemokines/growth factors involved in inflammatory/immune processes 
      were measured with a multiplex array system. The results have firstly highlighted 
      the different pattern of cytokine expression between the microenvironment at the 
      anterior surface of the eye and the systemic circulation. More importantly, the 
      kinetic of modulation of cytokines/chemokines at the conjunctival level following 
      PRK revealed that while the majority of cytokines/chemokines showed a significant 
      decrease, MCP-1 emerged in light of its pronounced and significant increase soon 
      after PRK and during the follow-up. This methodological approach has highlighted 
      the role of MCP-1 in the healing process following PRK and has shown a potential 
      for the identification of expression/modulation of soluble factors for biomarker 
      profiling in ocular surface diseases.
FAU - Tisato, Veronica
AU  - Tisato V
AD  - Department of Morphology, Surgery and Experimental Medicine and LTTA Centre, 
      University of Ferrara, Via Fossato di Mortara 66, 44121 Ferrara, Italy.
FAU - Perri, Paolo
AU  - Perri P
AD  - Department of Biomedical Sciences and Surgical Specialities, University-Hospital 
      of Ferrara, Corso Giovecca 203, 44121 Ferrara, Italy.
FAU - Rimondi, Erika
AU  - Rimondi E
AD  - Department of Life Sciences, University of Trieste, Via Manzoni 16, 34137 
      Trieste, Italy.
FAU - Melloni, Elisabetta
AU  - Melloni E
AD  - Department of Morphology, Surgery and Experimental Medicine and LTTA Centre, 
      University of Ferrara, Via Fossato di Mortara 66, 44121 Ferrara, Italy.
FAU - Lamberti, Giuseppe
AU  - Lamberti G
AD  - Department of Biomedical Sciences and Surgical Specialities, University-Hospital 
      of Ferrara, Corso Giovecca 203, 44121 Ferrara, Italy.
FAU - Milani, Daniela
AU  - Milani D
AD  - Department of Morphology, Surgery and Experimental Medicine and LTTA Centre, 
      University of Ferrara, Via Fossato di Mortara 66, 44121 Ferrara, Italy.
FAU - Secchiero, Paola
AU  - Secchiero P
AD  - Department of Morphology, Surgery and Experimental Medicine and LTTA Centre, 
      University of Ferrara, Via Fossato di Mortara 66, 44121 Ferrara, Italy.
FAU - Zauli, Giorgio
AU  - Zauli G
AD  - Institute for Maternal and Child Health, IRCCS Burlo Garofolo, Via dell'Istria 
      65/01, 34137 Trieste, Italy.
LA  - eng
PT  - Journal Article
DEP - 20151007
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
SB  - IM
MH  - Adult
MH  - Biomarkers/metabolism
MH  - Chemokine CCL2/metabolism
MH  - Chemokines/metabolism
MH  - Cohort Studies
MH  - Conjunctiva/metabolism/*pathology
MH  - Corneal Stroma/*metabolism
MH  - Cytokines/metabolism
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Inflammation/metabolism/*pathology
MH  - Inflammation Mediators/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Photorefractive Keratectomy/*methods
MH  - Refractive Errors/therapy
MH  - Wound Healing
PMC - PMC4617429
EDAT- 2015/11/04 06:00
MHDA- 2016/08/16 06:00
PMCR- 2015/10/07
CRDT- 2015/11/04 06:00
PHST- 2015/07/27 00:00 [received]
PHST- 2015/09/11 00:00 [revised]
PHST- 2015/09/15 00:00 [accepted]
PHST- 2015/11/04 06:00 [entrez]
PHST- 2015/11/04 06:00 [pubmed]
PHST- 2016/08/16 06:00 [medline]
PHST- 2015/10/07 00:00 [pmc-release]
AID - 10.1155/2015/942948 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2015;2015:942948. doi: 10.1155/2015/942948. Epub 2015 Oct 7.