PMID- 26525435
OWN - NLM
STAT- MEDLINE
DCOM- 20160907
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 5
DP  - 2015 Nov 3
TI  - Bio-grout based on microbially induced sand solidification by means of 
      asparaginase activity.
PG  - 16128
LID - 10.1038/srep16128 [doi]
LID - 16128
AB  - Bio-grout, a new ground improvement method, has been recently developed to 
      improve the mechanical properties, decrease the permeability of porous materials, 
      reinforce or repair cementitious materials and modify the properties of soil or 
      sand. Bio-grout production depends on microbially induced calcite precipitation 
      (MICP), which is driven mainly by an enzyme, urease. However, urease-based MICP 
      process produces excessive ammonia, in addition to secondary pollution generated 
      by urea that is used as substrate in it. In the present study, we reported 
      asparaginase-based MICP process for sand bio-grout development using Bacillus 
      megaterium, and results were also compared with urease-based bio-grouts. The 
      asparaginase activity led to significantly less ammonia production compared to 
      urease without compromising with desired properties of a novel grout. The UCS of 
      bio-grout was obtained at 980 kPa, while the permeability was decreased 
      substantially. The mineralogical composition of precipitated substance was 
      identified as calcite using XRD and the crystal morphology was observed under 
      SEM. The mass percentage of calcite in bio-grout was calculated by 
      thermogravimetric analysis and XCT verified calcite precipitation in it. The 
      results confirmed that biocalcification by means of bacterial asparaginase is a 
      potential solution for geotechnical problems. The asparaginase-based MICP process 
      could be of wider acceptance in future.
FAU - Li, Mengmeng
AU  - Li M
AD  - School of Ecological and Environmental Sciences, East China Normal University 
      Shanghai 200241, China.
FAU - Fu, Qing-Long
AU  - Fu QL
AD  - Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil 
      Science, Chinese Academy of Sciences, Nanjing, China.
FAU - Zhang, Qiuzhuo
AU  - Zhang Q
AD  - School of Ecological and Environmental Sciences, East China Normal University 
      Shanghai 200241, China.
FAU - Achal, Varenyam
AU  - Achal V
AD  - School of Ecological and Environmental Sciences, East China Normal University 
      Shanghai 200241, China.
FAU - Kawasaki, Satoru
AU  - Kawasaki S
AD  - Faculty of Engineering, Hokkaido University, Sapporo 0608628, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151103
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 7631-86-9 (Silicon Dioxide)
RN  - 7664-41-7 (Ammonia)
RN  - EC 3.5.1.1 (Asparaginase)
RN  - EC 3.5.1.5 (Urease)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Ammonia/metabolism
MH  - Asparaginase/*metabolism
MH  - Bacillus/enzymology
MH  - Calcium Carbonate/chemistry
MH  - Silicon Dioxide/*chemistry
MH  - Thermogravimetry
MH  - Tomography, X-Ray Computed
MH  - Urease/metabolism
PMC - PMC4630787
EDAT- 2015/11/04 06:00
MHDA- 2016/09/08 06:00
PMCR- 2015/11/03
CRDT- 2015/11/04 06:00
PHST- 2015/06/26 00:00 [received]
PHST- 2015/10/07 00:00 [accepted]
PHST- 2015/11/04 06:00 [entrez]
PHST- 2015/11/04 06:00 [pubmed]
PHST- 2016/09/08 06:00 [medline]
PHST- 2015/11/03 00:00 [pmc-release]
AID - srep16128 [pii]
AID - 10.1038/srep16128 [doi]
PST - epublish
SO  - Sci Rep. 2015 Nov 3;5:16128. doi: 10.1038/srep16128.